Abstract
Introgression of a high-molecular-weight glutenin subunit (HMW-GS) allele, 1Ay21∗, into commercial wheat cultivars increased overall grain protein content and bread-making quality, but the role of proteins beyond this HMW-GS itself was unknown. In addition to increased abundance of 1Ay HMW-GS, 115 differentially accumulated proteins (DAPs) were discovered between three cultivars and corresponding introgressed near-isogenic lines. Functional category analysis showed that the DAPs were predominantly other storage proteins and proteins involved in protein synthesis, protein folding, protein degradation, stress response, and grain development. Nearly half the genes encoding the DAPs showed strong coexpression patterns during grain development. Promoters of these genes are enriched in elements associated with transcription initiation and light response, indicating a potential connection between these cis-elements and grain protein accumulation. A model of how this HMW-GS enhances the abundance of machinery for protein synthesis and maturation during grain filling is proposed. This analysis not only provides insights into how introgression of the 1Ay21∗ improves grain protein content but also directs selection of protein candidates for future wheat quality breeding programs.
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Claims 7
Claim · model-written summary
Introgression of 1Ay21∗ increased wheat grain protein content.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- Introgression of a high-molecular-weight glutenin subunit (HMW-GS) allele, 1Ay21∗e0
- Gene
- 1Ay21∗e0
- Outcome
- overall grain protein contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introgression of a high-molecular-weight glutenin subunit (HMW-GS) allele, 1Ay21∗, into commercial wheat cultivars increased overall grain protein content and bread-making quality”
Claim · model-written summary
Introgression of 1Ay21∗ increased bread-making quality.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Introgression of a high-molecular-weight glutenin subunit (HMW-GS) allele, 1Ay21∗e0
- Gene
- 1Ay21∗e0
- Outcome
- bread-making qualitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introgression of a high-molecular-weight glutenin subunit (HMW-GS) allele, 1Ay21∗, into commercial wheat cultivars increased overall grain protein content and bread-making quality”
Claim · model-written summary
Introgressed wheat lines had increased 1Ay HMW-GS abundance.
- Taxon
- WheatTriticum aestivumt0
- Material
- introgressed near-isogenic linese0
- Gene
- 1Ay HMW-GSe0
- Outcome
- abundance of 1Ay HMW-GSe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to increased abundance of 1Ay HMW-GS, 115 differentially accumulated proteins (DAPs) were discovered between three cultivars and corresponding introgressed near-isogenic lines”
t0“Wheat”
Claim · model-written summary
The comparison found 115 differentially accumulated proteins.
- Taxon
- WheatTriticum aestivumt0
- Material
- introgressed near-isogenic linese0
- Outcome
- differentially accumulated proteinse0
- Value
- 115e0
- Comparator
- three cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“115 differentially accumulated proteins (DAPs) were discovered between three cultivars and corresponding introgressed near-isogenic lines”
t0“Wheat”
Claim · model-written summary
The differentially accumulated proteins were mainly storage and protein metabolism, stress response, and grain development proteins.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- other storage proteins and proteins involved in protein synthesis, protein folding, protein degradation, stress response, and grain developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the DAPs were predominantly other storage proteins and proteins involved in protein synthesis, protein folding, protein degradation, stress response, and grain development”
t0“Wheat”
Claim · model-written summary
Nearly half of DAP encoding genes showed strong coexpression during grain development.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- grain developmente0
- Outcome
- strong coexpression patternse0
- Value
- Nearly halfe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nearly half the genes encoding the DAPs showed strong coexpression patterns during grain development”
t0“Wheat”
Claim · model-written summary
Promoters of DAP encoding genes were enriched for transcription initiation and light response elements.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- elements associated with transcription initiation and light responsee0
- Direction
- enrichede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoters of these genes are enriched in elements associated with transcription initiation and light response”
t0“Wheat”
The same questions in other papers
transgenic · quality: general · Triticum aestivum (wheat)Positive and negative results
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transgenic · quality: protein · Triticum aestivum (wheat)Positive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.