Abstract
The experiment was conducted to develop an efficient protocol for in vitro regeneration of mungbean (Vignaradiata) on the aspect of regeneration potentiality of two mungbean varieties (BINA mung 5 and BINA mung 7) as influenced by different combinations of growth regulators supplemented with MS medium. Cotyledon explant of both varieties was used for the present study. Data were collected for various characters of callus initiation, shoot regeneration and root proliferation. Initiation of callus (%) and required days for its initiation and weight of callus were influenced significantly due to the effect of varieties where BINA mung 5 produced more callus induction (40.36%) at minimum requiring time (18.27 days) including heavier sizes of callus (1.54 g) than BINA mung 7 when BINA mung 5 further recorded the longest root (2.92 cm) compare to BINA mung 7. Effect of treatments of the present study were significantly influenced the whole characters regarding callus culture, shoot regeneration and root proliferation. The highest percentage of callus (88.44%) within minimum time (12.53 days) including larger sizes callus (3.521 g) were produced in 1.0 mg L1 BAP + 2.5 mg L1 NAA among the treatments while the highest percentage of regenerated shoot (83.44%) at minimum requiring time (17.59 days) and more shoots (7.69 callus1) were obtained in 1.0 mg L1 BAP + 2.0 mg L 1 NAA. Root induction (82.50%), number of roots plantlet1 (8.469) with minimum requiring time for initiation (14.13 days) and root length (5.250 cm) were the highest in 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAP. Incase of interaction, percentage of callus initiation (89.38 %) was the highest in BINA mung 5 treated by 1.0 mg L1 BAP + 2.5 mg L1 NAA at requiring minimum time (12.38 days) while same treatment produced the larger callus (3.581 g) among the interactions. The highest percentage (84.38%) and number (7.813 callus1) of shoot with minimum requiring time (17.50 days) were found from BINA mung 5 treated by 1.0 mg L1 BAP + 2.0 mg L1 NAA. Similarly, the longest shoot (5.58 cm) was produced from the BINA mung 5 treated by 1.0 mg L1 BAP + 2.0 mg L1 NAA. However, root induction (%), roots plantlet1, days required for root initiation and root length were statistically similar among the whole interaction treatments due to non significant variation. This result mentioned that the variety BINA mung 5 was better than BINA mung 7 for callus induction, shoot regeneration and root initiation while 1.0 mg L1 BAP + 2.5 mg L1 NAA, 1.0 mg L1 BAP + 2.0 mg L1 NAA and 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAP supplemented with MS medium were the best combinations for better callusing, higher ability of shoot regeneration and root proliferation.DOI: http://dx.doi.org/10.3329/jesnr.v7i2.22203 J. Environ. Sci. & Natural Resources, 7(2): 47-52 2014
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Claims 20
Claim · model-written summary
BINA mung 5 had higher callus induction than BINA mung 7, at 40.36%.
- Taxon
- mungbean
.jpg?width=60)
Vigna radiatae0 - Material
- BINA mung 5e1
- Outcome
- callus inductione1
- Direction
- moree1
- Value
- 40.36%e1
- Comparator
- BINA mung 7e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“mungbean (Vignaradiata)”
e1“BINA mung 5 produced more callus induction (40.36%) at minimum requiring time (18.27 days) including heavier sizes of callus (1.54 g) than BINA mung 7”
Claim · model-written summary
BINA mung 5 required fewer days for callus initiation than BINA mung 7, at 18.27 days.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Outcome
- requiring timee1
- Direction
- minimume1
- Value
- 18.27 dayse1
- Comparator
- BINA mung 7e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“BINA mung 5 produced more callus induction (40.36%) at minimum requiring time (18.27 days) including heavier sizes of callus (1.54 g) than BINA mung 7”
Claim · model-written summary
BINA mung 5 produced heavier callus than BINA mung 7, at 1.54 g.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Outcome
- sizes of calluse1
- Direction
- heaviere1
- Value
- 1.54 ge1
- Comparator
- BINA mung 7e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“BINA mung 5 produced more callus induction (40.36%) at minimum requiring time (18.27 days) including heavier sizes of callus (1.54 g) than BINA mung 7”
Claim · model-written summary
BINA mung 5 had the longest roots compared with BINA mung 7, at 2.92 cm.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Outcome
- roote1
- Direction
- longeste1
- Value
- 2.92 cme1
- Comparator
- BINA mung 7e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“BINA mung 5 further recorded the longest root (2.92 cm) compare to BINA mung 7”
Claim · model-written summary
Growth regulator treatments significantly influenced callus culture, shoot regeneration and root proliferation.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- treatmentse1
- Outcome
- callus culture, shoot regeneration and root proliferatione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“Effect of treatments of the present study were significantly influenced the whole characters regarding callus culture, shoot regeneration and root proliferation.”
Claim · model-written summary
1.0 mg L1 BAP + 2.5 mg L1 NAA gave the highest callus percentage, 88.44%.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 1.0 mg L1 BAP + 2.5 mg L1 NAAe1
- Outcome
- percentage of calluse1
- Direction
- higheste1
- Value
- 88.44%e1
- Comparator
- the treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“The highest percentage of callus (88.44%) within minimum time (12.53 days) including larger sizes callus (3.521 g) were produced in 1.0 mg L1 BAP + 2.5 mg L1 NAA among the treatments”
Claim · model-written summary
That BAP and NAA treatment minimized callus initiation time at 12.53 days.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 1.0 mg L1 BAP + 2.5 mg L1 NAAe1
- Outcome
- timee1
- Direction
- minimume1
- Value
- 12.53 dayse1
- Comparator
- the treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“The highest percentage of callus (88.44%) within minimum time (12.53 days) including larger sizes callus (3.521 g) were produced in 1.0 mg L1 BAP + 2.5 mg L1 NAA among the treatments”
Claim · model-written summary
That BAP and NAA treatment yielded larger callus, 3.521 g.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 1.0 mg L1 BAP + 2.5 mg L1 NAAe1
- Outcome
- sizes calluse1
- Direction
- largere1
- Value
- 3.521 ge1
- Comparator
- the treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“The highest percentage of callus (88.44%) within minimum time (12.53 days) including larger sizes callus (3.521 g) were produced in 1.0 mg L1 BAP + 2.5 mg L1 NAA among the treatments”
Claim · model-written summary
1.0 mg L1 BAP + 2.0 mg L 1 NAA yielded the highest shoot regeneration percentage, 83.44%.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 1.0 mg L1 BAP + 2.0 mg L 1 NAAe1
- Outcome
- percentage of regenerated shoote1
- Direction
- higheste1
- Value
- 83.44%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“the highest percentage of regenerated shoot (83.44%) at minimum requiring time (17.59 days) and more shoots (7.69 callus1) were obtained in 1.0 mg L1 BAP + 2.0 mg L 1 NAA”
Claim · model-written summary
That treatment minimized time for shoot regeneration at 17.59 days.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 1.0 mg L1 BAP + 2.0 mg L 1 NAAe1
- Outcome
- requiring timee1
- Direction
- minimume1
- Value
- 17.59 dayse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“the highest percentage of regenerated shoot (83.44%) at minimum requiring time (17.59 days) and more shoots (7.69 callus1) were obtained in 1.0 mg L1 BAP + 2.0 mg L 1 NAA”
Claim · model-written summary
That treatment produced more shoots, 7.69 per callus.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 1.0 mg L1 BAP + 2.0 mg L 1 NAAe1
- Outcome
- shootse1
- Direction
- moree1
- Value
- 7.69 callus1e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“the highest percentage of regenerated shoot (83.44%) at minimum requiring time (17.59 days) and more shoots (7.69 callus1) were obtained in 1.0 mg L1 BAP + 2.0 mg L 1 NAA”
Claim · model-written summary
0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAP gave highest root induction, 82.50%.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAPe1
- Outcome
- Root inductione1
- Direction
- higheste1
- Value
- 82.50%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“Root induction (82.50%), number of roots plantlet1 (8.469) with minimum requiring time for initiation (14.13 days) and root length (5.250 cm) were the highest in 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAP.”
Claim · model-written summary
That IAA, kinetin and BAP treatment gave 8.469 roots per plantlet.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAPe1
- Outcome
- number of roots plantlet1e1
- Direction
- higheste1
- Value
- 8.469e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“Root induction (82.50%), number of roots plantlet1 (8.469) with minimum requiring time for initiation (14.13 days) and root length (5.250 cm) were the highest in 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAP.”
Claim · model-written summary
That treatment minimized root initiation time at 14.13 days.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAPe1
- Outcome
- time for initiatione1
- Direction
- minimume1
- Value
- 14.13 dayse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“Root induction (82.50%), number of roots plantlet1 (8.469) with minimum requiring time for initiation (14.13 days) and root length (5.250 cm) were the highest in 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAP.”
Claim · model-written summary
That treatment yielded the highest root length, 5.250 cm.
- Taxon
- mungbeanVigna radiatae0
- Treatment
- 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAPe1
- Outcome
- root lengthe1
- Direction
- higheste1
- Value
- 5.250 cme1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“Root induction (82.50%), number of roots plantlet1 (8.469) with minimum requiring time for initiation (14.13 days) and root length (5.250 cm) were the highest in 0.2 mg L1 IAA + 1.0 mg L1 kinetin + 0.2 mg L1 BAP.”
Claim · model-written summary
BINA mung 5 with 1.0 mg L1 BAP + 2.5 mg L1 NAA yielded 89.38% callus initiation.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Treatment
- 1.0 mg L1 BAP + 2.5 mg L1 NAAe1
- Outcome
- percentage of callus initiatione1
- Direction
- higheste1
- Value
- 89.38 %e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“percentage of callus initiation (89.38 %) was the highest in BINA mung 5 treated by 1.0 mg L1 BAP + 2.5 mg L1 NAA at requiring minimum time (12.38 days) while same treatment produced the larger callus (3.581 g) among the interactions.”
Claim · model-written summary
The same variety and treatment minimized callus initiation time, 12.38 days.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Treatment
- 1.0 mg L1 BAP + 2.5 mg L1 NAAe1
- Outcome
- timee1
- Direction
- minimume1
- Value
- 12.38 dayse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“percentage of callus initiation (89.38 %) was the highest in BINA mung 5 treated by 1.0 mg L1 BAP + 2.5 mg L1 NAA at requiring minimum time (12.38 days) while same treatment produced the larger callus (3.581 g) among the interactions.”
Claim · model-written summary
The same variety and treatment produced larger callus, 3.581 g.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Treatment
- 1.0 mg L1 BAP + 2.5 mg L1 NAAe1
- Outcome
- calluse1
- Direction
- largere1
- Value
- 3.581 ge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“percentage of callus initiation (89.38 %) was the highest in BINA mung 5 treated by 1.0 mg L1 BAP + 2.5 mg L1 NAA at requiring minimum time (12.38 days) while same treatment produced the larger callus (3.581 g) among the interactions.”
Claim · model-written summary
BINA mung 5 with 1.0 mg L1 BAP + 2.0 mg L1 NAA had the highest shoot percentage, 84.38%.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Treatment
- 1.0 mg L1 BAP + 2.0 mg L1 NAAe1
- Outcome
- percentagee1
- Direction
- higheste1
- Value
- 84.38%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“The highest percentage (84.38%) and number (7.813 callus1) of shoot with minimum requiring time (17.50 days) were found from BINA mung 5 treated by 1.0 mg L1 BAP + 2.0 mg L1 NAA.”
Claim · model-written summary
The same variety and treatment produced 7.813 shoots per callus and required 17.50 days.
- Taxon
- mungbeanVigna radiatae0
- Material
- BINA mung 5e1
- Treatment
- 1.0 mg L1 BAP + 2.0 mg L1 NAAe1
- Outcome
- numbere1
- Direction
- higheste1
- Value
- 7.813 callus1e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mungbean (Vignaradiata)”
e1“The highest percentage (84.38%) and number (7.813 callus1) of shoot with minimum requiring time (17.50 days) were found from BINA mung 5 treated by 1.0 mg L1 BAP + 2.0 mg L1 NAA.”
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