Abstract
The cell-to-cell movement of Turnip crinkle virus (TCV) in Nicotiana benthamiana requires the presence of its coat protein (CP), a known suppressor of RNA silencing. RNA transcripts of a TCV construct containing a reporter gene (green fluorescent protein) (TCV-sGFP) in place of the CP open reading frame generated foci of three to five cells. TCV CP delivered in trans by Agrobacterium tumefaciens infiltration potentiated movement of TCV-sGFP and increased foci diameter, on average, by a factor of four. Deletion of the TCV movement proteins in TCV-sGFP (construct TCVDelta92-sGFP) abolished the movement complementation ability of TCV CP. Other known suppressors of RNA silencing from a wide spectrum of viruses also complemented the movement of TCV-sGFP when delivered in trans by Agrobacterium tumefaciens. These include suppressors from nonplant viruses with no known plant movement function, demonstrating that this assay is based solely on RNA silencing suppression. While the TCV-sGFP construct is primarily used as an infectious RNA transcript, it was also subcloned for direct expression from Agrobacterium tumefaciens for simple quantification of suppressor activity based on fluorescence levels in whole leaves. Thus, this system provides the flexibility to assay for suppressor activity in either the cytoplasm or nucleus, depending on the construct employed.
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Claims 4
Claim · model-written summary
TCV coat protein delivered in trans increased TCV-sGFP foci diameter by a factor of four in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- TCV CP delivered in transe1
- Interactor
- Turnip crinkle virusTurnip crinkle viruse0pathogen
- Outcome
- foci diametere1
- Direction
- increasede1
- Value
- a factor of foure1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell-to-cell movement of Turnip crinkle virus (TCV) in Nicotiana benthamiana requires the presence of its coat protein (CP)”
e1“TCV CP delivered in trans by Agrobacterium tumefaciens infiltration potentiated movement of TCV-sGFP and increased foci diameter, on average, by a factor of four”
Claim · model-written summary
TCV coat protein delivered in trans potentiated cell-to-cell movement of TCV-sGFP in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- TCV CP delivered in transe1
- Outcome
- movement of TCV-sGFPe1
- Direction
- potentiatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell-to-cell movement of Turnip crinkle virus (TCV) in Nicotiana benthamiana requires the presence of its coat protein (CP)”
e1“TCV CP delivered in trans by Agrobacterium tumefaciens infiltration potentiated movement of TCV-sGFP and increased foci diameter, on average, by a factor of four”
Claim · model-written summary
Deleting the TCV movement proteins abolished the movement complementation ability of TCV coat protein.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- Deletion of the TCV movement proteinse2
- Outcome
- movement complementation ability of TCV CPe2
- Direction
- abolishede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell-to-cell movement of Turnip crinkle virus (TCV) in Nicotiana benthamiana requires the presence of its coat protein (CP)”
e2“Deletion of the TCV movement proteins in TCV-sGFP (construct TCVDelta92-sGFP) abolished the movement complementation ability of TCV CP”
Claim · model-written summary
Other known RNA silencing suppressors from a wide range of viruses also complemented TCV-sGFP movement when delivered in trans.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- Other known suppressors of RNA silencinge3
- Outcome
- movement of TCV-sGFPe3
- Direction
- complementede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell-to-cell movement of Turnip crinkle virus (TCV) in Nicotiana benthamiana requires the presence of its coat protein (CP)”
e3“Other known suppressors of RNA silencing from a wide spectrum of viruses also complemented the movement of TCV-sGFP when delivered in trans by Agrobacterium tumefaciens”
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9 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.