Abstract
A new sweet protein, named mabinlin II, was extracted with 0.5 M NaCl solution from the seeds of Capparis masaikai Lévl. and purified by ammonium sulfate fractionation, carboxymethylcellulose-Sepharose ion-exchange chromatography and gel filtration. The sweetness of mabinlin II was unchanged by at least 48 h incubation at nearly boiling temperature. Purified mabinlin II thus obtained gave a single band having a molecular mass of 14 kDa on SDS/PAGE. In the presence of dithiothreitol, mabinlin II gave two bands having molecular masses of 4.6 kDa and 5.2 kDa on SDS/PAGE. Two peptides (A chain and B chain) were separated from reduced and S-carboxamidomethylated mabinlin II by HPLC. The amino acid sequences of the A chain and B chain were determined by the automatic Edman-degradation method. The A chain and B chain consist of 33-amino-acid and 72-amino-acid residues, respectively. The A chain is mostly composed of hydrophilic amino acid residues and the B chain also contains many hydrophilic residues. High similarity was found between the amino acid sequences of mabinlin II and 2S seed storage proteins, especially 2S albumin AT2S3 in Arabidopsis thaliana (mouse-ear cress).
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Claims 10
Claim · model-written summary
Mabinlin II sweetness was unchanged after prolonged near-boiling incubation.
Verbatim
e0“The sweetness of mabinlin II was unchanged by at least 48 h incubation at nearly boiling temperature”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
Purified mabinlin II appeared as a 14 kDa band.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Gene
- mabinlin IIe0
- Outcome
- molecular masse0
- Value
- 14 kDae0
Verbatim
e0“Purified mabinlin II thus obtained gave a single band having a molecular mass of 14 kDa on SDS/PAGE”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
With dithiothreitol, mabinlin II produced a 4.6 kDa band.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Gene
- mabinlin IIe0
- Outcome
- molecular massese0
- Value
- 4.6 kDae0
Verbatim
e0“In the presence of dithiothreitol, mabinlin II gave two bands having molecular masses of 4.6 kDa and 5.2 kDa on SDS/PAGE”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
With dithiothreitol, mabinlin II produced a 5.2 kDa band.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Gene
- mabinlin IIe0
- Outcome
- molecular massese0
- Value
- 5.2 kDae0
Verbatim
e0“In the presence of dithiothreitol, mabinlin II gave two bands having molecular masses of 4.6 kDa and 5.2 kDa on SDS/PAGE”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
The reduced protein yielded separate A and B chains.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Gene
- mabinlin IIe0
- Outcome
- Two peptides (A chain and B chain)e0
Verbatim
e0“Two peptides (A chain and B chain) were separated from reduced and S-carboxamidomethylated mabinlin II by HPLC”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
The amino acid sequences of both chains were determined.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Outcome
- amino acid sequences of the A chain and B chaine0
Not reportedComparatorValue
Verbatim
e0“The amino acid sequences of the A chain and B chain were determined by the automatic Edman-degradation method”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
The A chain has 33 amino acid residues.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Gene
- A chaine0
- Outcome
- amino-acid residuese0
- Value
- 33-amino-acide0
Verbatim
e0“The A chain and B chain consist of 33-amino-acid and 72-amino-acid residues, respectively”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
The B chain has 72 amino acid residues.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Gene
- B chaine0
- Outcome
- amino-acid residuese0
- Value
- 72-amino-acide0
Verbatim
e0“The A chain and B chain consist of 33-amino-acid and 72-amino-acid residues, respectively”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
Both chains contain many hydrophilic residues.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Outcome
- hydrophilic amino acid residuese0
Verbatim
e0“The A chain is mostly composed of hydrophilic amino acid residues and the B chain also contains many hydrophilic residues”
e1“from the seeds of Capparis masaikai Lévl.”
Claim · model-written summary
Mabinlin II sequence was highly similar to 2S seed storage proteins, especially Arabidopsis AT2S3.
- Taxon
- Capparis masaikai Lévl.Capparis masaikaie1
- Gene
- mabinlin IIe0
- Outcome
- High similaritye0
- Comparator
- 2S seed storage proteinse0
Not reportedDirection
Verbatim
e0“High similarity was found between the amino acid sequences of mabinlin II and 2S seed storage proteins, especially 2S albumin AT2S3 in Arabidopsis thaliana (mouse-ear cress)”
e1“from the seeds of Capparis masaikai Lévl.”