Abstract
Brome mosaic virus (BMV) is a positive-sense RNA plant virus, the tripartite genomic RNAs of which are separately packaged into virions. RNA3 is copackaged with subgenomic RNA4. In barley protoplasts coinoculated with RNA1 and RNA2, an RNA3 mutant with a 69-nucleotide (nt) deletion in the 3'-proximal region of the 3a open reading frame (ORF) was very poorly packaged compared with other RNA3 mutants and wild-type RNA3, despite their comparable accumulation in the absence of coat protein. Computer analysis of RNA secondary structure predicted two stem-loop (SL) structures (i.e., SL-I and SL-II) in the 69-nt region. Disruption of SL-II, but not of SL-I, significantly reduced RNA3 packaging. A chimeric BMV RNA3 (B3Cmp), with the BMV 3a ORF replacing that of cucumber mosaic virus (CMV), was packaged negligibly, whereas RNA4 was packaged efficiently. Replacement of the 3'-proximal region of the CMV 3a ORF in B3Cmp with the 3'-proximal region of the BMV 3a ORF significantly improved packaging efficiency, and the disruption of SL-II in the substituted BMV 3a ORF region greatly reduced packaging efficiency. These results suggest that the 3'-proximal region of the BMV 3a ORF, especially SL-II predicted between nt 904 and 933, plays an important role in the packaging of BMV RNA3 in vivo. Furthermore, the efficient packaging of RNA4 without RNA3 in B3Cmp-infected cells implies the presence of an element in the 3a ORF of BMV RNA3 that regulates the copackaging of RNA3 and RNA4.
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Claims 10
Claim · model-written summary
In barley protoplasts, a BMV RNA3 mutant with a 69-nt deletion in the 3-proximal region of the 3a ORF was very poorly packaged compared with other RNA3 mutants and wild-type RNA3.
- Taxon
- Barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- 69-nucleotide (nt) deletion in the 3'-proximal region of the 3a open reading frame (ORF)e0
- Interactor
- Brome Mosaic Virus
Brome mosaic virust0pathogen - Outcome
- packagede0
- Direction
- very poorly packagede0
- Comparator
- other RNA3 mutants and wild-type RNA3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“In barley protoplasts coinoculated with RNA1 and RNA2, an RNA3 mutant with a 69-nucleotide (nt) deletion in the 3'-proximal region of the 3a open reading frame (ORF) was very poorly packaged compared with other RNA3 mutants and wild-type RNA3”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
Computer analysis of RNA secondary structure predicted two stem-loop structures (SL-I and SL-II) in the 69-nt region.
- Taxon
- BarleyHordeum vulgaret0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- two stem-loop (SL) structurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Computer analysis of RNA secondary structure predicted two stem-loop (SL) structures (i.e., SL-I and SL-II) in the 69-nt region”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
Disruption of SL-II significantly reduced RNA3 packaging.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Disruption of SL-IIe0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- RNA3 packaginge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of SL-II, but not of SL-I, significantly reduced RNA3 packaging”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
Disruption of SL-I did not significantly reduce RNA3 packaging.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- SL-Ie0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- RNA3 packaginge0
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of SL-II, but not of SL-I, significantly reduced RNA3 packaging”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
A chimeric BMV RNA3 (B3Cmp) with the BMV 3a ORF replacing that of cucumber mosaic virus was packaged negligibly.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- chimeric BMV RNA3 (B3Cmp)e0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- packagede0
- Direction
- negligiblye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A chimeric BMV RNA3 (B3Cmp), with the BMV 3a ORF replacing that of cucumber mosaic virus (CMV), was packaged negligibly”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
RNA4 was packaged efficiently in the presence of the chimeric B3Cmp.
- Taxon
- BarleyHordeum vulgaret0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- packaged efficientlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA4 was packaged efficiently”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
Replacing the 3-proximal region of the CMV 3a ORF in B3Cmp with the 3-proximal region of the BMV 3a ORF significantly improved packaging efficiency.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Replacement of the 3'-proximal region of the CMV 3a ORF in B3Cmp with the 3'-proximal region of the BMV 3a ORFe0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- packaging efficiencye0
- Direction
- improvede0
- Comparator
- B3Cmpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Replacement of the 3'-proximal region of the CMV 3a ORF in B3Cmp with the 3'-proximal region of the BMV 3a ORF significantly improved packaging efficiency”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
Disruption of SL-II in the substituted BMV 3a ORF region greatly reduced packaging efficiency.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- disruption of SL-II in the substituted BMV 3a ORF regione0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- packaging efficiencye0
- Direction
- greatly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the disruption of SL-II in the substituted BMV 3a ORF region greatly reduced packaging efficiency”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
The authors conclude that the 3-proximal region of the BMV 3a ORF, especially SL-II predicted between nt 904 and 933, plays an important role in BMV RNA3 packaging in vivo.
- Taxon
- BarleyHordeum vulgaret0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- packaging of BMV RNA3e0
- Qualifier
- SL-II predicted between nt 904 and 933e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 3'-proximal region of the BMV 3a ORF, especially SL-II predicted between nt 904 and 933, plays an important role in the packaging of BMV RNA3 in vivo”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
Claim · model-written summary
The authors infer that efficient packaging of RNA4 without RNA3 in B3Cmp-infected cells implies an element in the BMV RNA3 3a ORF that regulates copackaging of RNA3 and RNA4.
- Taxon
- BarleyHordeum vulgaret0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Outcome
- copackaging of RNA3 and RNA4e0
- Qualifier
- presence of an element in the 3a ORF of BMV RNA3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the efficient packaging of RNA4 without RNA3 in B3Cmp-infected cells implies the presence of an element in the 3a ORF of BMV RNA3 that regulates the copackaging of RNA3 and RNA4”
t0“Brome Mosaic Virus RNA3 in Barley Protoplasts”
The same questions in other papers
bromine atom · Brome mosaic virus · Hordeum vulgare (barley)Positive and negative results
2 in the opposite direction
- negative
- negative
loss of function · Brome mosaic virus · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.