Abstract
The 3' portions of plus-strand brome mosaic virus (BMV) RNAs mimic cellular tRNAs. Nucleotide substitutions or deletions in the 3'CCA of the tRNA-like sequence (TLS) affect minus-strand initiation unless repaired. We observed that 2-nucleotide deletions involving the CCA 3' sequence in one or all BMV RNAs still allowed RNA accumulation in barley protoplasts at significant levels. Alterations of CCA to GGA in only BMV RNA3 also allowed RNA accumulation at wild-type levels. However, substitutions in all three BMV RNAs severely reduced RNA accumulation, demonstrating that substitutions have different repair requirements than do small deletions. Furthermore, wild-type BMV RNA1 was required for the repair and replication of RNAs with nucleotide substitutions. Results from sequencing of progeny viral RNA from mutant input RNAs demonstrated that RNA1 did not contribute its sequence to the mutant RNAs. Instead, the repaired ends were heterogeneous, with one-third having a restored CCA and others having sequences with the only commonality being the restoration of one cytidylate. The role of BMV RNA1 in increased repair was examined.
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Claims 7
Claim · model-written summary
Two nucleotide CCA deletions in one or all BMV RNAs still permitted significant RNA accumulation.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- 2-nucleotide deletions involving the CCA 3' sequencee0
- Interactor
- BMV
Brome mosaic viruse0pathogen - Outcome
- BMV RNAs still allowed RNA accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“2-nucleotide deletions involving the CCA 3' sequence in one or all BMV RNAs still allowed RNA accumulation in barley protoplasts at significant levels”
Claim · model-written summary
Changing CCA to GGA in BMV RNA3 alone permitted wild type RNA accumulation.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Alterations of CCA to GGA in only BMV RNA3e0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- RNA accumulatione0
- Direction
- wild-type levelse0
- Comparator
- wild-type levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alterations of CCA to GGA in only BMV RNA3 also allowed RNA accumulation at wild-type levels.”
e1“barley protoplasts”
Claim · model-written summary
Substitutions in all three BMV RNAs severely reduced RNA accumulation.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- substitutions in all three BMV RNAse0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- RNA accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“substitutions in all three BMV RNAs severely reduced RNA accumulation”
e1“barley protoplasts”
Claim · model-written summary
Wild type BMV RNA1 was required to repair and replicate substitution mutant RNAs.
- Taxon
- barleyHordeum vulgaree1
- Interactor
- BMVBrome mosaic viruse0pathogen
- Gene
- BMV RNA1e0
- Outcome
- repair and replication of RNAs with nucleotide substitutionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wild-type BMV RNA1 was required for the repair and replication of RNAs with nucleotide substitutions”
e1“barley protoplasts”
Claim · model-written summary
Sequencing showed that BMV RNA1 did not donate sequence to mutant RNAs.
- Taxon
- barleyHordeum vulgaree1
- Interactor
- BMVBrome mosaic viruse2pathogen
- Gene
- RNA1e0
- Outcome
- did not contribute its sequence to the mutant RNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA1 did not contribute its sequence to the mutant RNAs”
e1“barley protoplasts”
e2“one or all BMV RNAs”
Claim · model-written summary
One third of repaired viral ends restored CCA.
- Taxon
- barleyHordeum vulgaree2
- Interactor
- BMVBrome mosaic viruse3pathogen
- Outcome
- restored CCAe0
- Value
- one-thirde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one-third having a restored CCA”
e1“the repaired ends were heterogeneous”
e2“barley protoplasts”
e3“one or all BMV RNAs”
Claim · model-written summary
Other repaired viral ends shared restoration of one cytidylate.
- Taxon
- barleyHordeum vulgaree1
- Interactor
- BMVBrome mosaic viruse2pathogen
- Outcome
- restoration of one cytidylatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“others having sequences with the only commonality being the restoration of one cytidylate”
e1“barley protoplasts”
e2“one or all BMV RNAs”
The same questions in other papers
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1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.