Abstract
One of several genes coding for the major pea storage protein, legumin, has been completely sequenced. The sequence covers the whole of the transcribed region, plus 5' and 3' untranscribed sequences. The predicted protein sequence starts with a signal peptide and is followed by the legumin alpha polypeptide sequence of 36. 44kd and the beta polypeptide sequence of 20. 19kd . Compared to other legume storage proteins, the alpha and beta polypeptide sequences encoded by this legumin gene, which contain 3 met and 5 cys residues, are relatively rich in the sulphur amino acids. The coding sequence is interrupted by three introns which show boundary sequences typical of higher plant genes. The 5' end of the gene sequence contains a 'TATA box', a ' CAAT box' and a sequence showing some homology to an ' AGGA box'. An extra sequence, identical to the normal polyadenylation signal of the legumin message is seen in the 3' untranscribed region. The structure of the gene and the possible significance of secondary structures in the nascent RNA transcript in affecting the choice of polyadenylation site is discussed.
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Claims 8
Claim · model-written summary
A pea legumin gene was completely sequenced, spanning transcribed and flanking regions.
- Taxon
- pea
Lathyrus oleraceuse0 - Gene
- legumine0
- Outcome
- completely sequencede0
- Value
- 5' and 3'e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced. The sequence covers the whole of the transcribed region, plus 5' and 3' untranscribed sequences.”
Claim · model-written summary
The predicted protein begins with a signal peptide.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- legumine1
- Outcome
- starts with a signal peptidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted protein sequence starts with a signal peptide”
e1“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced.”
Claim · model-written summary
The legumin alpha polypeptide was reported at 36. 44kd.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- legumine0
- Outcome
- alpha polypeptide sequencee0
- Value
- 36. 44kde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the legumin alpha polypeptide sequence of 36. 44kd”
e1“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced.”
Claim · model-written summary
The legumin beta polypeptide was reported at 20. 19kd.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- legumine1
- Outcome
- beta polypeptide sequencee0
- Value
- 20. 19kde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the beta polypeptide sequence of 20. 19kd”
e1“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced.”
Claim · model-written summary
Legumin alpha and beta polypeptides were relatively rich in sulfur amino acids and contained 3 methionine and 5 cysteine residues.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- legumine0
- Outcome
- relatively rich in the sulphur amino acidse0
- Value
- 3 met and 5 cys residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the alpha and beta polypeptide sequences encoded by this legumin gene, which contain 3 met and 5 cys residues, are relatively rich in the sulphur amino acids.”
e1“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced.”
Claim · model-written summary
The coding sequence was interrupted by three introns with typical plant gene boundaries.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- legumine1
- Outcome
- coding sequence is interrupted by three intronse0
- Value
- three intronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The coding sequence is interrupted by three introns which show boundary sequences typical of higher plant genes.”
e1“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced.”
Claim · model-written summary
The 5-prime region contained TATA, CAAT and AGGA-like boxes.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- legumine1
- Outcome
- 'TATA box', a ' CAAT box' and a sequence showing some homology to an ' AGGA box'e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 5' end of the gene sequence contains a 'TATA box', a ' CAAT box' and a sequence showing some homology to an ' AGGA box'.”
e1“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced.”
Claim · model-written summary
An additional polyadenylation-signal-identical sequence occurred in the 3-prime untranslated region.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- legumine0
- Outcome
- extra sequence, identical to the normal polyadenylation signale0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An extra sequence, identical to the normal polyadenylation signal of the legumin message is seen in the 3' untranscribed region.”
e1“One of several genes coding for the major pea storage protein, legumin, has been completely sequenced.”