Abstract
Using quantitative X-ray microanalysis in combination with CeCl3-based cytochemical staining of hydrogen peroxide (H2O2) we have developed a new solution for quantification of H2O2 at the subcellular level. Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv. showed that the obtained cerium precipitates by CeCl3 staining were the mixture of cerium perhydroxides and cerium phosphate, in which the fractions of CePO4 were: (1) 52-74% in cell walls of fresh leaf segments, and (2) 34-70% in the cytoplasm in 10 mM H2O2-treated leaf segments that were previously freeze-dried. Taking the concentration of cerium phosphate as staining background, we reached the cellular concentration of cerium perhydroxides and the corresponding concentration of H2O2. Results showed that H2O2 was present in the cytoplasm of rehydrated leaf segments (29-58 mM), but in fresh leaves, H2O2 was observed in the walls of all measured cell types (17-74 mM).
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Claims 5
Claim · model-written summary
H2O2 was present in the cytoplasm of rehydrated leaf segments at 29-58 mM.
- Taxon
- Populus euphratica
Populus euphraticae1 - Organ / stage
- cytoplasme2
- Treatment
- rehydrated leaf segmentse2
- Outcome
- H2O2e2
- Value
- 29-58 mMe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv.”
e2“Results showed that H2O2 was present in the cytoplasm of rehydrated leaf segments (29-58 mM), but in fresh leaves, H2O2 was observed in the walls of all measured cell types (17-74 mM)”
Claim · model-written summary
In fresh leaves, H2O2 was present in the walls of all measured cell types at 17-74 mM.
- Taxon
- Populus euphraticaPopulus euphraticae1
- Organ / stage
- walls of all measured cell typese2
- Treatment
- fresh leavese2
- Outcome
- H2O2e2
- Value
- 17-74 mMe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv.”
e2“Results showed that H2O2 was present in the cytoplasm of rehydrated leaf segments (29-58 mM), but in fresh leaves, H2O2 was observed in the walls of all measured cell types (17-74 mM)”
Claim · model-written summary
Cerium-based staining with X-ray microanalysis quantifies H2O2 at the subcellular level, using cerium phosphate as the staining background.
- Taxon
- Populus euphraticaPopulus euphraticae1
- Outcome
- quantification of H2O2 at the subcellular levele5
- Comparator
- concentration of cerium phosphate as staining backgrounde4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv.”
e4“Taking the concentration of cerium phosphate as staining background”
e5“we have developed a new solution for quantification of H2O2 at the subcellular level”
Claim · model-written summary
The CePO4 fraction of the cerium precipitate in cell walls of fresh leaf segments was 52-74%.
- Taxon
- Populus euphraticaPopulus euphraticae1
- Organ / stage
- cell wallse3
- Treatment
- fresh leaf segmentse3
- Outcome
- fractions of CePO4e3
- Value
- 52-74%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv.”
e3“in which the fractions of CePO4 were: (1) 52-74% in cell walls of fresh leaf segments, and (2) 34-70% in the cytoplasm in 10 mM H2O2-treated leaf segments that were previously freeze-dried”
Claim · model-written summary
The CePO4 fraction of the cerium precipitate in the cytoplasm of 10 mM H2O2-treated, previously freeze-dried leaf segments was 34-70%.
- Taxon
- Populus euphraticaPopulus euphraticae1
- Organ / stage
- cytoplasme3
- Treatment
- 10 mM H2O2-treated leaf segments that were previously freeze-driede3
- Outcome
- fractions of CePO4e3
- Value
- 34-70%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv.”
e3“in which the fractions of CePO4 were: (1) 52-74% in cell walls of fresh leaf segments, and (2) 34-70% in the cytoplasm in 10 mM H2O2-treated leaf segments that were previously freeze-dried”