Abstract
Certain actively filling plant sucrose sinks such as a seed, a tuber, or a root can be identified by measuring the uridine diphosphate and pyrophosphate-dependent metabolism of sucrose. Sucrolysis in both active and quiescent sucrose sinks was tested and sucrose synthase was found to be the predominant sucrose breakdown activity. Sucrolysis via invertases was low and secondary in both types of sinks. Sucrose synthase activity dropped markedly, greater than fivefold, in quiescent sinks. The tests are consistent with the hypothesis that the sucrose filling activity, i.e. the sink strength, of these plant sinks can be measured by testing the uridine diphosphate and pyrophosphate-dependent breakdown of sucrose. Measuring the initial reactions of sucrolysis shows much promise for use in agriculture crop and tree improvement research as a biochemical test for sink strength.
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Claims 5
Claim · model-written summary
Sucrose synthase was the predominant sucrose breakdown activity in both active and quiescent sucrose sinks.
Verbatim
e0“Certain actively filling plant sucrose sinks such as a seed, a tuber, or a root can be identified”
e1“Sucrolysis in both active and quiescent sucrose sinks was tested and sucrose synthase was found to be the predominant sucrose breakdown activity”
Claim · model-written summary
Sucrolysis via invertases was low and secondary in both active and quiescent sucrose sinks.
Verbatim
e0“Certain actively filling plant sucrose sinks such as a seed, a tuber, or a root can be identified”
e2“Sucrolysis via invertases was low and secondary in both types of sinks”
Claim · model-written summary
Sucrose synthase activity dropped more than fivefold in quiescent sucrose sinks.
Verbatim
e0“Certain actively filling plant sucrose sinks such as a seed, a tuber, or a root can be identified”
e3“Sucrose synthase activity dropped markedly, greater than fivefold, in quiescent sinks”
Claim · model-written summary
Measuring UDP- and PPi-dependent sucrose breakdown is consistent with a test of sink strength (sucrose filling activity) in plant sinks.
Verbatim
e1“Sucrolysis in both active and quiescent sucrose sinks was tested”
e4“The tests are consistent with the hypothesis that the sucrose filling activity, i.e. the sink strength, of these plant sinks can be measured by testing the uridine diphosphate and pyrophosphate-dependent breakdown of sucrose”
Claim · model-written summary
Initial reactions of sucrolysis show promise as a biochemical test for sink strength in crop and tree improvement.
Verbatim
e5“Measuring the initial reactions of sucrolysis shows much promise for use in agriculture crop and tree improvement research as a biochemical test for sink strength”