Abstract
국내 개발 재배품종인 코스피드 이탈리안 라이그라스의 최적 배양조건을 확립하기 위하여 성숙종자로부터 캘러스 유도조건 및 식물체 재분화 조건을 조사하였다. 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 NAA 보다 효율적이었으며, 5 mg/L 2,4-D가 첨가된 N6 배지에서 캘러스가 가장 높은 빈도로 유도되었다. 캘러스로부터 식물체 재분화는 1 mg/L 2,4-D와 5mg/L BA가 첨가된 N6 배지에서 배양했을 때 67% 이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스 유도와 식물체 재분화에는 N6배지가 효과적이었으며, 재분화배지에 첨가되는 탄소원에는 30g/L의 sucrose가 가장 높은 재분화 효율을 나타내었다. 본 연구를 통하여 확립된 고효율 재분화 시스템은 분자육종을 통한 신품종 이탈리안라이그라스의 실용화 연구에 큰 도움이 될 것으로 추측된다. In order to improve forage characteristics of Italian ryegrass by genetic transformation, an efficient callus induction from mature seed and optimal plant regeneration system were established using a domestic cultivar 'Kospeed'. Addition of 5 mg/L of 2,4-D showed highest frequency of embryogenic callus induction from mature seeds. N6 medium showed higher frequency of both callus induction and plant regeneration as compared with MS and SH medium. The highest plant regeneration frequency 67% was obtained when embryogenic calli were transferred to N6 medium containing 1 mg/L 2,4-D and 5 mg/L BA. Supplementation of regeneration medium with sucrose at 30 g/L level maximized regeneration frequency as compared to the other concentrations. These data would be very helpful for molecular breeding of domestic Italian ryegrass cultivar through genetic transformation.
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Claims 5
Claim · model-written summary
Adding 5 mg/L 2,4-D gave the highest frequency of embryogenic callus induction from mature Italian ryegrass seeds.
- Taxon
- Italian Ryegrass
Lolium multiflorumt0 - Organ / stage
- mature seedse0
- Treatment
- 5 mg/L of 2,4-De0
- Outcome
- embryogenic callus inductione0
- Direction
- higheste0
Not reportedMaterialComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 5 mg/L of 2,4-D showed highest frequency of embryogenic callus induction from mature seeds.”
t0“Italian Ryegrass Cultivar”
Claim · model-written summary
N6 medium gave higher callus induction frequency than MS and SH medium.
- Taxon
- Italian RyegrassLolium multiflorumt0
- Treatment
- N6 mediume0
- Outcome
- callus inductione0
- Direction
- highere0
- Comparator
- MS and SH mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N6 medium showed higher frequency of both callus induction and plant regeneration as compared with MS and SH medium.”
t0“Italian Ryegrass Cultivar”
Claim · model-written summary
N6 medium gave higher plant regeneration frequency than MS and SH medium.
- Taxon
- Italian RyegrassLolium multiflorumt0
- Treatment
- N6 mediume0
- Outcome
- plant regeneratione0
- Direction
- highere0
- Comparator
- MS and SH mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N6 medium showed higher frequency of both callus induction and plant regeneration as compared with MS and SH medium.”
t0“Italian Ryegrass Cultivar”
Claim · model-written summary
Embryogenic calli on N6 medium with 1 mg/L 2,4-D and 5 mg/L BA had the highest plant regeneration frequency, 67%.
- Taxon
- Italian RyegrassLolium multiflorumt0
- Organ / stage
- embryogenic callie0
- Treatment
- N6 medium containing 1 mg/L 2,4-D and 5 mg/L BAe0
- Outcome
- plant regeneration frequencye0
- Direction
- higheste0
- Value
- 67%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest plant regeneration frequency 67% was obtained when embryogenic calli were transferred to N6 medium containing 1 mg/L 2,4-D and 5 mg/L BA.”
t0“Italian Ryegrass Cultivar”
Claim · model-written summary
Supplementing regeneration medium with 30 g/L sucrose maximized regeneration frequency relative to other concentrations.
- Taxon
- Italian RyegrassLolium multiflorumt0
- Treatment
- sucrose at 30 g/L levele0
- Outcome
- regeneration frequencye0
- Direction
- maximizede0
- Comparator
- the other concentrationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supplementation of regeneration medium with sucrose at 30 g/L level maximized regeneration frequency as compared to the other concentrations.”
t0“Italian Ryegrass Cultivar”
The same questions in other papers
2,4-D · growth · Lolium multiflorum (Italian ryegrass)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Lolium multiflorum (Italian ryegrass)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.