Abstract
Attempts have been made to establish protocol for in vitro propagation of Momordica tuberosa (Cogn) Roxb. using nodal segments and shoot apices obtained from field-grown mature plants. In vitro regeneration was achieved from nodal explants on Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine at 2.22, 4.40, 6.62, and 8.90 μM and kinetin at 2.32, 4.60, 6.92, and 9.30 μM either alone or in combination (BA + Kn). Within the ranges evaluated, the regeneration medium containing 4.40 μM BA combined with 4.60 μM Kn showed highest regeneration efficiency, with 9.0 ± 0.49 shoots per explant. Such in vitro regenerated shoots attained a maximum length of 10.0 ± 0.47 cm. The regeneration medium containing BA + Kn was used to subculture regenerated shoots at 4-week intervals. BA at 13.30 μM induced regeneration from shoot apex cultures, and 75% of explants showed regeneration efficiency with 7.8 ± 0.66 cm as the mean length of shoot. Such shoots could be subcultured on medium containing BA. Microshoots were rooted onto MS medium supplemented with 4.90 μM indole-3-butyric acid. Regenerated plants were established in the greenhouse with 90% survival rate. The protocol is simple, rapid, and efficient for in vitro propagation of nodal explants and shoot apices of M. tuberosa.
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Claims 7
Claim · model-written summary
Combined 4.40 μM BA and 4.60 μM Kn gave the highest nodal regeneration, with 9.0 ± 0.49 shoots per explant.
- Treatment
- 4.40 μM BA combined with 4.60 μM Kne0
- Outcome
- regeneration efficiencye0
- Direction
- higheste0
- Value
- 9.0 ± 0.49 shoots per explante0
Not reportedTaxonComparator
Verbatim
e0“regeneration medium containing 4.40 μM BA combined with 4.60 μM Kn showed highest regeneration efficiency, with 9.0 ± 0.49 shoots per explant”
Claim · model-written summary
Regenerated shoots reached a maximum length of 10.0 ± 0.47 cm.
Not reportedTaxon
Verbatim
e0“in vitro regenerated shoots attained a maximum length of 10.0 ± 0.47 cm”
Claim · model-written summary
At 13.30 μM, BA induced shoot-apex regeneration in 75% of explants.
Not reportedTaxonComparator
Verbatim
e0“BA at 13.30 μM induced regeneration from shoot apex cultures, and 75% of explants showed regeneration efficiency”
Claim · model-written summary
Shoot-apex regenerated shoots had a mean length of 7.8 ± 0.66 cm.
Not reportedTaxon
Verbatim
e0“7.8 ± 0.66 cm as the mean length of shoot”
Claim · model-written summary
Microshoots rooted on MS medium supplemented with 4.90 μM indole-3-butyric acid.
- Treatment
- MS medium supplemented with 4.90 μM indole-3-butyric acide0
- Outcome
- Microshoots were rootede0
Not reportedTaxon
Verbatim
e0“Microshoots were rooted onto MS medium supplemented with 4.90 μM indole-3-butyric acid.”
Claim · model-written summary
Regenerated plants established in a greenhouse with 90% survival.
Not reportedTaxon
Verbatim
e0“Regenerated plants were established in the greenhouse with 90% survival rate.”
Claim · model-written summary
The protocol was characterized as simple, rapid and efficient for nodal and shoot-apex propagation.
Not reportedComparatorValue
Verbatim
e0“The protocol is simple, rapid, and efficient for in vitro propagation of nodal explants and shoot apices of M. tuberosa.”