Abstract
Abstract Phosphoenolpyruvate, which is the substrate for the enzyme for the enzyme pyruvate kinase, is also hydrolysed by a phosphatase when the former enzyme is assayed in unpurified extracts from plant tissues. The activity of this phosphatase has been examined in extracts of cucumber leaves from plants receiving a wide range of N, P, K and Mg treatments. When assayed in the presence of added MgSO 4 , the phosphatase activity was highly correlated with the P content in the leaves and was not affected by the variation in the N, K or Mg content in the leaves. While the pyruvate kinase activity in extracts of cucumber leaves can be used to indicate the K or Mg status of the plants, the associated phosphatase activity gives an indication of the P status. These enzyme assays which can be completed within 2 h, could offer a rapid means of assessment of the K, Mg and P status of cucumber plants.
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Claims 6
Claim · model-written summary
Phosphoenolpyruvate was also hydrolysed by a phosphatase in unpurified plant extracts.
- Taxon
- cucumber
Cucumis sativust0 - Organ / stage
- plant tissuese0
- Gene
- a phosphatasee0
- Outcome
- Phosphoenolpyruvatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phosphoenolpyruvate, which is the substrate for the enzyme for the enzyme pyruvate kinase, is also hydrolysed by a phosphatase when the former enzyme is assayed in unpurified extracts from plant tissues.”
t0“A phosphatase as a potential indicator of the phosphorus status of the glasshouse cucumber (Cucumis sativus)”
Claim · model-written summary
With added MgSO 4, phosphatase activity was highly correlated with cucumber leaf P content.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- leavese0
- Gene
- phosphatasee0
- Compound
- Pe0
- Outcome
- phosphatase activitye0
- Qualifier
- in the presence of added MgSO 4e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When assayed in the presence of added MgSO 4 , the phosphatase activity was highly correlated with the P content in the leaves”
t0“A phosphatase as a potential indicator of the phosphorus status of the glasshouse cucumber (Cucumis sativus)”
Claim · model-written summary
Phosphatase activity was unaffected by variation in cucumber leaf N, K, or Mg.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- leavese0
- Compound
- N, K or Mge0
- Outcome
- phosphataset0
- Direction
- not affectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was not affected by the variation in the N, K or Mg content in the leaves”
t0“A phosphatase as a potential indicator of the phosphorus status of the glasshouse cucumber (Cucumis sativus)”
Claim · model-written summary
Cucumber leaf pyruvate kinase activity can indicate plant K or Mg status.
- Taxon
- cucumberCucumis sativuse0
- Gene
- pyruvate kinasee0
- Outcome
- K or Mg statuse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pyruvate kinase activity in extracts of cucumber leaves can be used to indicate the K or Mg status of the plants”
Claim · model-written summary
Associated phosphatase activity indicates cucumber P status.
- Taxon
- cucumberCucumis sativust0
- Gene
- phosphatasee0
- Outcome
- P statuse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the associated phosphatase activity gives an indication of the P status”
t0“A phosphatase as a potential indicator of the phosphorus status of the glasshouse cucumber (Cucumis sativus)”
Claim · model-written summary
Combined enzyme assays could rapidly assess cucumber K, Mg, and P status within 2 h.
- Taxon
- cucumberCucumis sativuse0
- Outcome
- K, Mg and P statuse0
- Value
- 2 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These enzyme assays which can be completed within 2 h, could offer a rapid means of assessment of the K, Mg and P status of cucumber plants.”