Abstract
Abstract Tobacco rattle virus (TRV) was detected by PCR in potato tubers and in root samples. Oligonucleotide primers were synthesized to nucleotides of the 16 k protein gene of RNA‐1 of the TRV strain ‘SYM’. After phenol extraction of the total nucleic acids of the samples and reverse transcription. the PCR reaction mixture was subjected to 25 cycles and the amplification product was detected by electrophoresis. TRV RNA was found in roots and in freshly harvested tubers in September, as well as in tubers which were stored until March. This indicates that there was no elimination of TRV during the storage period. The field samples of potato cv. Désirée tested in September were symptomlessly infected. whereas the stored tubers of various cultivars showed, internal symptoms. Nucleic acids extracts of latently‐infected and spraing‐affected tubers inoculated on tobacco plants led to local necrotic rings. Nucleic acids from infected tobacco cv. Samsun plants led to systemic symptoms as well. TRV was identified by ISEM and ELISA only in the systemically‐infected tobacco plants, but not in the locally infected tobacco plants. This suggests the presence of the ‘NM’ type of TRV in tubers, which produces only RNA‐1 but no complete virus particles.
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Claims 8
Claim · model-written summary
PCR detected TRV in potato tubers and roots.
- Taxon
- potato
Solanum tuberosume0 - Interactor
- Tobacco rattle virusTobacco rattle viruse0pathogen
- Outcome
- TRVe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco rattle virus (TRV) was detected by PCR in potato tubers and in root samples.”
Claim · model-written summary
TRV RNA occurred in roots and freshly harvested tubers in September and in tubers stored until March.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- rootse0
- Interactor
- TRVTobacco rattle viruse0pathogen
- Outcome
- TRV RNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TRV RNA was found in roots and in freshly harvested tubers in September, as well as in tubers which were stored until March.”
t0“Potato”
e1“Tobacco rattle virus (TRV) was detected by PCR in potato tubers and in root samples.”
Claim · model-written summary
Potato cv. Désirée field samples were symptomlessly infected in September.
- Taxon
- potatoSolanum tuberosume0
- Material
- Désiréee0
- Outcome
- symptomlessly infectede0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The field samples of potato cv. Désirée tested in September were symptomlessly infected.”
Claim · model-written summary
Stored potato tubers of various cultivars showed internal symptoms.
- Taxon
- PotatoSolanum tuberosumt0
- Organ / stage
- stored tuberse0
- Outcome
- internal symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the stored tubers of various cultivars showed, internal symptoms.”
t0“Potato”
Claim · model-written summary
Nucleic acid extracts from infected tubers caused local necrotic rings on tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Nucleic acids extracts of latently‐infected and spraing‐affected tuberse0
- Outcome
- local necrotic ringse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nucleic acids extracts of latently‐infected and spraing‐affected tubers inoculated on tobacco plants led to local necrotic rings.”
Claim · model-written summary
Nucleic acids from infected tobacco cv. Samsun caused systemic symptoms.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- Samsune0
- Treatment
- Nucleic acids from infected tobacco cv. Samsun plantse0
- Outcome
- systemic symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nucleic acids from infected tobacco cv. Samsun plants led to systemic symptoms as well.”
Claim · model-written summary
ISEM and ELISA identified TRV in systemically infected tobacco plants but not locally infected plants.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- TRVTobacco rattle viruse0pathogen
- Outcome
- TRVe0
- Comparator
- locally infected tobacco plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TRV was identified by ISEM and ELISA only in the systemically‐infected tobacco plants, but not in the locally infected tobacco plants.”
Claim · model-written summary
The findings suggest tubers contain the NM type of TRV producing RNA-1 but no complete particles.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- TRVTobacco rattle viruse0pathogen
- Outcome
- presence of the ‘NM’ type of TRV in tuberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This suggests the presence of the ‘NM’ type of TRV in tubers, which produces only RNA‐1 but no complete virus particles.”
t0“Potato”
The same questions in other papers
infection · disease · Nicotiana tabacum (tobacco)Positive and negative results
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- positive
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.