Abstract
Four physiologically and phenotypically diversified tobacco (Nicotiana tabacum L. cv. Samsun) plantlet variants had been generated by cultivation on media either lacking or containing sucrose (0 and 3 %, m/v) under two different photon flux densities (PFD), 50 µmol m-2 s-1 (LL) and 200 µmol m-2 s-1 (HL). Plantlets were transferred into soil without any pre-acclimation and grown either under PFD of 200 µmol m-2 s-1 or 700 µmol m-2 s-1. Sucrose feeding in vitro resulted in reduced degree and duration of wilting after transfer. The highest readiness for ex vitro acclimation was found in 3 % HL plants, in which changes of photosynthetic apparatus and stress responses were the smallest. On the contrary, the steepest decline of Fv/Fm ratio on the first day after transplantation, doubled chlorophyll content and almost tripled D1/LHC 2 ratio after 7 d of ex vitro growth under 700 µmol m-2 s-1 characterized 0 % HL plants, which had suffered chronic photoinhibition in vitro. Remarkably high abscisic acid content at the end of in vitro cultivation and during acclimation as well as increased synthesis of both D1 and LHC 2 proteins even at the end of analyzed acclimation period were found only in 0 % LL plants. Increase of D1/LHC 2 ratio and chlorophyll contents demonstrate that in vitro developed leaves of all plant variants are able to acclimate to new environment. The most surprising result in the whole study is the drop of D1 protein synthesis in all plants on the 3rd day. Five times decline of photoprotection level of xanthophylls in plants after ex vitro transfer into the same PFD showed stress character of in vitro cultures.
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Claims 12
Claim · model-written summary
In vitro sucrose feeding reduced wilting after transfer.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- Sucrose feeding in vitroe0
- Outcome
- degree and duration of wiltinge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose feeding in vitro resulted in reduced degree and duration of wilting after transfer.”
t0“Tobacco”
Claim · model-written summary
The 3 % HL plantlets had the highest readiness for ex vitro acclimation.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 3 % HL plantse0
- Outcome
- readiness for ex vitro acclimatione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest readiness for ex vitro acclimation was found in 3 % HL plants”
t0“Tobacco”
Claim · model-written summary
Changes of the photosynthetic apparatus and stress responses were smallest in 3 % HL plantlets.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 3 % HL plantse1
- Outcome
- changes of photosynthetic apparatus and stress responsese0
- Direction
- smalleste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in which changes of photosynthetic apparatus and stress responses were the smallest.”
t0“Tobacco”
e1“3 % HL plants”
Claim · model-written summary
The 0 % HL plantlets had the steepest first-day decline in Fv/Fm ratio after transplantation.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 0 % HL plantse1
- Outcome
- Fv/Fm ratioe0
- Direction
- steepest declinee0
- Qualifier
- on the first day after transplantatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the steepest decline of Fv/Fm ratio on the first day after transplantation”
t0“Tobacco”
e1“0 % HL plants”
Claim · model-written summary
Chlorophyll content doubled in 0 % HL plantlets after 7 days of ex vitro growth at 700 µmol m-2 s-1.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 0 % HL plantse1
- Outcome
- chlorophyll contente0
- Direction
- doublede0
- Value
- doublede0
- Qualifier
- after 7 d of ex vitro growth under 700 µmol m-2 s-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“doubled chlorophyll content and almost tripled D1/LHC 2 ratio after 7 d of ex vitro growth under 700 µmol m-2 s-1”
t0“Tobacco”
e1“0 % HL plants”
Claim · model-written summary
D1/LHC 2 ratio almost tripled in 0 % HL plantlets after 7 days of ex vitro growth at 700 µmol m-2 s-1.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 0 % HL plantse1
- Outcome
- D1/LHC 2 ratioe0
- Direction
- almost triplede0
- Value
- almost triplede0
- Qualifier
- after 7 d of ex vitro growth under 700 µmol m-2 s-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“almost tripled D1/LHC 2 ratio after 7 d of ex vitro growth under 700 µmol m-2 s-1”
t0“Tobacco”
e1“0 % HL plants”
Claim · model-written summary
The 0 % LL plantlets had remarkably high abscisic acid content at the end of in vitro cultivation and during acclimation.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 0 % LL plantse1
- Outcome
- abscisic acid contente0
- Direction
- Remarkably highe0
- Qualifier
- at the end of in vitro cultivation and during acclimatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Remarkably high abscisic acid content at the end of in vitro cultivation and during acclimation”
t0“Tobacco”
e1“0 % LL plants”
Claim · model-written summary
D1 and LHC 2 protein synthesis increased only in 0 % LL plantlets, persisting through the analyzed acclimation period.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 0 % LL plantse0
- Gene
- D1 and LHC 2 proteinse0
- Outcome
- synthesis of both D1 and LHC 2 proteinse0
- Direction
- increasede0
- Qualifier
- even at the end of analyzed acclimation periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased synthesis of both D1 and LHC 2 proteins even at the end of analyzed acclimation period were found only in 0 % LL plants.”
t0“Tobacco”
Claim · model-written summary
D1/LHC 2 ratio increased in leaves of all tobacco plantlet variants after transfer.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- in vitro developed leavese0
- Outcome
- D1/LHC 2 ratioe0
- Direction
- Increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increase of D1/LHC 2 ratio and chlorophyll contents demonstrate that in vitro developed leaves of all plant variants are able to acclimate to new environment.”
t0“Tobacco”
Claim · model-written summary
Chlorophyll content increased in leaves of all tobacco plantlet variants after transfer.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- in vitro developed leavese0
- Outcome
- chlorophyll contentse0
- Direction
- Increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increase of D1/LHC 2 ratio and chlorophyll contents demonstrate that in vitro developed leaves of all plant variants are able to acclimate to new environment.”
t0“Tobacco”
Claim · model-written summary
D1 protein synthesis dropped in all plantlets on day 3.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- D1 proteine0
- Outcome
- D1 protein synthesise0
- Direction
- drope0
- Qualifier
- on the 3rd daye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the drop of D1 protein synthesis in all plants on the 3rd day.”
t0“Tobacco”
Claim · model-written summary
Photoprotection by xanthophylls fell fivefold after transfer into the same photon flux density.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- ex vitro transfer into the same PFDe0
- Outcome
- photoprotection level of xanthophyllse0
- Direction
- declinee0
- Value
- Five timese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five times decline of photoprotection level of xanthophylls in plants after ex vitro transfer into the same PFD”
t0“Tobacco”
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