Abstract
Microspore-derived embryos of Brassica napus were cryopreserved in liquid nitrogen (LN) after desiccation treatment. Desiccated embryos which were directly immersed in LN converted to plantlets without any decrease in their viability. In contrast, non-desiccated embryos lost their viability after cryopreservation. The longevity and viability of the desiccated embryos of B. campestris were examined under several storage conditions. Survival rates of the embryos were affected by temperature of storage. Lower temperature conditions prolonged their viability. Storage up to 3 years at -80°C did not affect the plant conversion ability of desiccated embryos. These results suggest that naked desiccated embryos of Brassica spp. could be stored for a long period at lower-temperature.
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Claims 4
Claim · model-written summary
Desiccated Brassica napus embryos converted to plantlets after liquid-nitrogen immersion without a viability decrease.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Treatment
- directly immersed in LNe0
- Outcome
- converted to plantletse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Desiccated embryos which were directly immersed in LN converted to plantlets without any decrease in their viability.”
t0“Brassica spp.”
e1“Microspore-derived embryos of Brassica napus”
Claim · model-written summary
Non-desiccated Brassica napus embryos lost viability after cryopreservation.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- cryopreservatione0
- Outcome
- viabilitye0
- Direction
- loste0
- Comparator
- desiccated embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“non-desiccated embryos lost their viability after cryopreservation”
e1“Microspore-derived embryos of Brassica napus were cryopreserved in liquid nitrogen (LN) after desiccation treatment.”
Claim · model-written summary
Lower storage temperatures prolonged viability of B. campestris embryos.
- Taxon
- B. campestris
.jpg?width=60)
Brassica rapa subsp. oleiferae1 - Treatment
- Lower temperature conditionse0
- Outcome
- viabilitye0
- Direction
- prolongede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Lower temperature conditions prolonged their viability.”
e1“The longevity and viability of the desiccated embryos of B. campestris were examined under several storage conditions.”
Claim · model-written summary
Storage for up to 3 years at -80°C left plant conversion ability of B. campestris embryos unchanged.
- Taxon
- B. campestrisBrassica rapa subsp. oleiferae1
- Treatment
- Storage up to 3 years at -80°Ce0
- Outcome
- plant conversion abilitye0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Storage up to 3 years at -80°C did not affect the plant conversion ability of desiccated embryos.”
e1“The longevity and viability of the desiccated embryos of B. campestris were examined under several storage conditions.”
The same questions in other papers
in vitro · growth · Brassica napus (oilseed rape)Positive and negative results
6 papers in the same direction as this paper · 30 in the opposite direction
- positive
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See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.