Abstract
Marker-free transgenic cucumber (Cucumis sativus L.) cv. Poinsett 76 SR plants were produced by Agrobacterium mediated transformation. A transformation efficiency of 1.62 was observed on using Agrobacterium tumefaciens strain LBA4404 harbouring Arabidopsis cbf1 gene driven by the inducible promoter RD29A in a binary vector system pCAMBIA. Transgene integration and single copy insert in transgenic cucumber was confirmed by polymerase chain reaction (PCR) and Southern blot analysis in T0 lines and also confirmed marker-free status in T1 generation. Transgene expression was confirmed by reverse transcription (RT)-PCR in T1 generation transgenic cucumber and advanced to T2 generation. Upon exposure to chilling stress (4 °C), the T2 generation transgenic plants survived up to 36 h; however, wild-type plants could not survive and gradually died. A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress. Thus, the transgenic cucumber plants expressing Arabidopsis cbf1 gene conferred protection against chilling stress.
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Claims 8
Claim · model-written summary
Agrobacterium transformation produced marker-free transgenic cucumber with efficiency 1.62.
- Taxon
- cucumber
Cucumis sativust0 - Interactor
- Agrobacterium tumefaciens strain LBA4404
Agrobacterium tumefaciense0other - Gene
- Arabidopsis cbf1 genee0
- Outcome
- transformation efficiencye0
- Value
- 1.62e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A transformation efficiency of 1.62 was observed on using Agrobacterium tumefaciens strain LBA4404 harbouring Arabidopsis cbf1 gene driven by the inducible promoter RD29A in a binary vector system pCAMBIA”
t0“cucumber”
Claim · model-written summary
The transgene was integrated as a single copy and T1 cucumber was marker free.
- Taxon
- cucumberCucumis sativuse0
- Outcome
- Transgene integration and single copy inserte0
- Qualifier
- marker-free status in T1 generatione0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgene integration and single copy insert in transgenic cucumber was confirmed by polymerase chain reaction (PCR) and Southern blot analysis in T0 lines and also confirmed marker-free status in T1 generation”
Claim · model-written summary
Transgene expression was confirmed in T1 cucumber.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- T1 generatione0
- Outcome
- Transgene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgene expression was confirmed by reverse transcription (RT)-PCR in T1 generation transgenic cucumber”
Claim · model-written summary
T2 transgenic cucumber survived chilling for up to 36 h whereas wild-type plants died.
- Taxon
- cucumberCucumis sativust0
- Treatment
- chilling stress (4 °C)e0
- Outcome
- survivede0
- Value
- up to 36 he0
- Comparator
- wild-type plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon exposure to chilling stress (4 °C), the T2 generation transgenic plants survived up to 36 h; however, wild-type plants could not survive and gradually died”
t0“cucumber”
Claim · model-written summary
Transgenic cucumber had a significantly lower membrane injury index than wild type under chilling.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- transgenic cucumbere0
- Outcome
- membrane injury index (MII)e0
- Direction
- decreasee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress”
Claim · model-written summary
Transgenic cucumber had significantly higher antioxidant enzyme activities under chilling.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- transgenic cucumbere0
- Outcome
- activities of antioxidant enzymes (SOD and CAT)e0
- Direction
- increasee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress”
Claim · model-written summary
Transgenic cucumber had significantly higher free proline content under chilling.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- transgenic cucumbere0
- Outcome
- free proline contente0
- Direction
- increasee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress”
Claim · model-written summary
Transgenic cucumber had significantly higher leaf relative water content under chilling.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- leavese0
- Treatment
- transgenic cucumbere0
- Outcome
- relative water content (RWC)e0
- Direction
- increasee0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant decrease in membrane injury index (MII), increase in activities of antioxidant enzymes (SOD and CAT), free proline content and relative water content (RWC) in the leaves were observed in transgenic cucumber as compared to wild-type under chilling stress”