Abstract
Nicotiana tabacum cv. Xanthi nn plants were transformed with nucleotides 3472-4916 of tobacco mosaic virus (TMV) strain U1. This sequence contains all but the three 3 terminal nucleotides of the TMV 54-kDa gene, which encodes a putative component of the replicase complex. These plants were resistant to infection when challenged with either TMV U1 virions or TMV U1 RNA at concentrations of up to 500 micrograms/ml or 300 micrograms/ml, respectively, the highest concentrations tested. Resistance was also exhibited when plants were inoculated at 100 micrograms/ml with the closely related TMV mutant YSI/1 but was not shown in plants challenged at the same concentrations with the more distantly related TMV strains U2 or L or cucumber mosaic virus. Although the copy number of the 54-kDa gene sequence varied in individual transformants from 1 to approximately 5, the level of resistance in plants was not dependent on the number of copies of the 54-kDa gene sequence integrated. The transformed plants accumulated a 54-kDa gene sequence-specific RNA transcript of the expected size, but no protein product was detected.
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Claims 7
Claim · model-written summary
Transformed tobacco resisted TMV U1 virions and RNA at the highest tested concentrations.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Material
- cv. Xanthi nne0
- Treatment
- transformed with nucleotides 3472-4916 of tobacco mosaic virus (TMV) strain U1e0
- Interactor
- tobacco mosaic virus (TMV) strain U1
Tobacco mosaic viruse0pathogen - Outcome
- resistant to infectione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana tabacum cv. Xanthi nn plants were transformed with nucleotides 3472-4916 of tobacco mosaic virus (TMV) strain U1.”
e1“These plants were resistant to infection when challenged with either TMV U1 virions or TMV U1 RNA at concentrations of up to 500 micrograms/ml or 300 micrograms/ml, respectively, the highest concentrations tested.”
Claim · model-written summary
Transformed tobacco resisted the closely related TMV mutant YSI/1.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- inoculated at 100 micrograms/mle1
- Interactor
- TMV mutant YSI/1e1pathogen
- Outcome
- Resistancee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana tabacum cv. Xanthi nn plants”
e1“Resistance was also exhibited when plants were inoculated at 100 micrograms/ml with the closely related TMV mutant YSI/1”
Claim · model-written summary
Transformed tobacco did not resist the more distant TMV strains U2 or L.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Interactor
- TMV strains U2 or LTobacco mosaic viruse1pathogen
- Outcome
- was not showne1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana tabacum cv. Xanthi nn plants”
e1“was not shown in plants challenged at the same concentrations with the more distantly related TMV strains U2 or L”
Claim · model-written summary
Transformed tobacco did not resist cucumber mosaic virus.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Interactor
- cucumber mosaic virus
cucumber mosaic cucumoviruse1pathogen - Outcome
- was not showne1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana tabacum cv. Xanthi nn plants”
e1“was not shown in plants challenged at the same concentrations with the more distantly related TMV strains U2 or L or cucumber mosaic virus”
Claim · model-written summary
Resistance was independent of integrated 54-kDa gene copy number.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Gene
- 54-kDa genee1
- Outcome
- level of resistancee1
- Direction
- not dependente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana tabacum cv. Xanthi nn plants”
e1“the level of resistance in plants was not dependent on the number of copies of the 54-kDa gene sequence integrated”
Claim · model-written summary
Transformed tobacco accumulated a transcript of the expected size.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Gene
- 54-kDa genee1
- Outcome
- 54-kDa gene sequence-specific RNA transcript of the expected sizee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana tabacum cv. Xanthi nn plants”
e1“The transformed plants accumulated a 54-kDa gene sequence-specific RNA transcript of the expected size, but no protein product was detected.”
Claim · model-written summary
No protein product was detected in transformed tobacco.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Outcome
- no protein product was detectede1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana tabacum cv. Xanthi nn plants”
e1“but no protein product was detected”
The same questions in other papers
nucleotide · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- negative
transgenic · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.