Abstract
The transcription factor SHE1 was induced by tobacco mosaic virus (TMV) infection in tobacco cv. Samsun NN (SNN) and SHE1 inhibited TMV accumulation when expressed constitutively. To better understand the role of SHE1 in virus infection, transgenic SNN tobacco plants generated to over-express SHE1 (OEx-SHE1) or silence expression of SHE1 (si-SHE1) were infected with TMV. OEx-SHE1 affected the local lesion resistance response to TMV, whereas si-SHE1 did not. However, si-SHE1 allowed a slow systemic infection to occur in SNN tobacco. An inhibitor of virus replication (IVR) was known to reduce the accumulation of TMV in SNN tobacco. Analysis of SHE1 and IVR mRNA levels in OEx-SHE1 plants showed constitutive expression of both mRNAs, whereas both mRNAs were less expressed in si-SHE1 plants, even after TMV infection, indicating that SHE1 and IVR were associated with a common signaling pathway. SHE1 and IVR interacted with each other in four different assay systems. The yeast two-hybrid assay also delimited sequences required for the interaction of these two proteins to the SHE1 central 58-79% region and the IVR C-terminal 50% of the protein sequences. This suggests that SHE is a transcription factor involved in the induction of IVR and that IVR binds to SHE1 to regulate its own synthesis.
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Claims 11
Claim · model-written summary
TMV infection induced SHE1 in SNN tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- Samsun NNe0
- Treatment
- tobacco mosaic virus (TMV) infectione0
- Interactor
- tobacco mosaic virus (TMV)
Tobacco mosaic viruse0pathogen - Gene
- SHE1e0
- Outcome
- SHE1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcription factor SHE1 was induced by tobacco mosaic virus (TMV) infection in tobacco cv. Samsun NN (SNN)”
Claim · model-written summary
Constitutive SHE1 expression inhibited TMV accumulation.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- expressed constitutivelye0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- SHE1e0
- Outcome
- TMV accumulatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHE1 inhibited TMV accumulation when expressed constitutively.”
t0“Tobacco”
Claim · model-written summary
SHE1 overexpression affected the local lesion resistance response to TMV.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- OEx-SHE1e0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- SHE1e0
- Outcome
- local lesion resistance response to TMVe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OEx-SHE1 affected the local lesion resistance response to TMV, whereas si-SHE1 did not.”
t0“Tobacco”
Claim · model-written summary
SHE1 silencing did not affect the local lesion resistance response to TMV.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- si-SHE1e0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- SHE1e0
- Outcome
- local lesion resistance response to TMVe0
- Direction
- did note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OEx-SHE1 affected the local lesion resistance response to TMV, whereas si-SHE1 did not.”
t0“Tobacco”
Claim · model-written summary
SHE1 silencing allowed slow systemic TMV infection in SNN tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- SNNe0
- Treatment
- si-SHE1e0
- Interactor
- TMVTobacco mosaic viruse1pathogen
- Gene
- SHE1e0
- Outcome
- slow systemic infectione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“si-SHE1 allowed a slow systemic infection to occur in SNN tobacco.”
e1“tobacco mosaic virus (TMV) infection in tobacco cv. Samsun NN (SNN)”
t0“The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in N Gene Tobacco”
Claim · model-written summary
SHE1 overexpressing plants constitutively expressed SHE1 and IVR mRNAs.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- OEx-SHE1e0
- Gene
- SHE1 and IVRe1
- Outcome
- constitutive expression of both mRNAse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OEx-SHE1 plants showed constitutive expression of both mRNAs”
e1“SHE1 and IVR interacted with each other in four different assay systems.”
t0“The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in N Gene Tobacco”
Claim · model-written summary
SHE1 silencing reduced SHE1 and IVR mRNA expression even after TMV infection.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- si-SHE1e0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- SHE1 and IVRe1
- Outcome
- both mRNAse0
- Direction
- less expressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both mRNAs were less expressed in si-SHE1 plants, even after TMV infection”
e1“SHE1 and IVR interacted with each other in four different assay systems.”
t0“The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in N Gene Tobacco”
Claim · model-written summary
SHE1 and IVR interacted in four assay systems.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- SHE1 and IVRe0
- Outcome
- interacted with each othere0
- Value
- four different assay systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHE1 and IVR interacted with each other in four different assay systems.”
t0“Tobacco”
Claim · model-written summary
Protein interaction required the SHE1 central 58-79% region.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- centrale0
- Gene
- SHE1e0
- Outcome
- sequences required for the interactione0
- Value
- 58-79%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sequences required for the interaction of these two proteins to the SHE1 central 58-79% region and the IVR C-terminal 50% of the protein sequences.”
t0“Tobacco”
Claim · model-written summary
Protein interaction required the IVR C-terminal 50% region.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- IVRe0
- Outcome
- sequences required for the interactione0
- Value
- 50%e0
- Qualifier
- C-terminale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sequences required for the interaction of these two proteins to the SHE1 central 58-79% region and the IVR C-terminal 50% of the protein sequences.”
t0“Tobacco”
Claim · model-written summary
The authors propose SHE1 induces IVR and IVR binding regulates its own synthesis.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- IVRe0
- Outcome
- regulate its own synthesise0
- Qualifier
- IVR binds to SHE1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This suggests that SHE is a transcription factor involved in the induction of IVR and that IVR binds to SHE1 to regulate its own synthesis.”
t0“Tobacco”
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