Paper · 1975 · Biotechnology and Bioengineering

Production of plant virus inhibitor by Phytolacca americana suspension culture

Masanaru Misawa; Mineyuki Hayashi; Hozumi Tanaka; et al.

5
claims
6
verbatim quotes
15
citations
2
research areas
—
Not open access

Abstract

The inhibitory activity of tobacco mosaic virus (TMV) infection was assayed with the extracts of various callus tissues derived from the intact plants. Phytolacca americana callus was selected as a producer of the virus inhibitor and its cultural conditions in suspension were examined for cell growth and the inhibitor production. A modified liquid medium containing twofold concentrations of all components in that of Murashige and Skoog plus2,4-D (1.0 mg/liter) and sucrose (6%), but without any vitamins and glycine was chosen for production of higher levels of the inhibitor. TMV infections in tobacco, bean, and tomato plants were markedly inhibited by the introduction of the disrupted whole broth of suspension cultured P. americana.

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Claims 5

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Phytolacca americana callus was selected as a producer of a virus inhibitor.

Taxon
Phytolacca americanaPhytolacca americanae0
Organ / stage
calluse0
Outcome
producer of the virus inhibitore0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phytolacca americana
Phytolacca americana · TaxonNY State IPM Program at Cornell University from New York, USA · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Phytolacca americana callus was selected as a producer of the virus inhibitor”

✓ located verbatim in the source · abstract, characters 152–229 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A modified liquid medium produced higher levels of inhibitor in Phytolacca americana suspension culture.

Taxon
Phytolacca americanaPhytolacca americanat0
Treatment
A modified liquid medium containing twofold concentrations of all components in that of Murashige and Skoog plus2,4-D (1.0 mg/liter) and sucrose (6%), but without any vitamins and glycinee0
Outcome
levels of the inhibitore0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phytolacca americana
Phytolacca americana · TaxonNY State IPM Program at Cornell University from New York, USA · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“A modified liquid medium containing twofold concentrations of all components in that of Murashige and Skoog plus2,4-D (1.0 mg/liter) and sucrose (6%), but without any vitamins and glycine was chosen for production of higher levels of the inhibitor.”

✓ located verbatim in the source · abstract, characters 332–580 · show in text ↑
t0

“Phytolacca americana”

✓ located verbatim in the source · title, characters 39–59 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Disrupted Phytolacca americana suspension culture broth markedly inhibited TMV infection in tobacco.

Treatment
introduction of the disrupted whole broth of suspension cultured P. americanae0
Interactor
TMVTobacco mosaic viruse0pathogen
Outcome
TMV infectionse0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗
Tobacco mosaic virus
Tobacco mosaic virus · InteractorFrank Vincentz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“TMV infections in tobacco, bean, and tomato plants were markedly inhibited by the introduction of the disrupted whole broth of suspension cultured P. americana.”

✓ located verbatim in the source · abstract, characters 581–741 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Disrupted Phytolacca americana suspension culture broth markedly inhibited TMV infection in bean.

Taxon
beane0
Treatment
introduction of the disrupted whole broth of suspension cultured P. americanae0
Interactor
TMVTobacco mosaic viruse0pathogen
Outcome
TMV infectionse0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Tobacco mosaic virus
Tobacco mosaic virus · InteractorFrank Vincentz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“TMV infections in tobacco, bean, and tomato plants were markedly inhibited by the introduction of the disrupted whole broth of suspension cultured P. americana.”

✓ located verbatim in the source · abstract, characters 581–741 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Disrupted Phytolacca americana suspension culture broth markedly inhibited TMV infection in tomato.

Treatment
introduction of the disrupted whole broth of suspension cultured P. americanae0
Interactor
TMVTobacco mosaic viruse0pathogen
Outcome
TMV infectionse0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Tobacco mosaic virus
Tobacco mosaic virus · InteractorFrank Vincentz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“TMV infections in tobacco, bean, and tomato plants were markedly inhibited by the introduction of the disrupted whole broth of suspension cultured P. americana.”

✓ located verbatim in the source · abstract, characters 581–741 · show in text ↑

loss of function · Tobacco mosaic virus · Solanum lycopersicum (tomato)Positive and negative results

5 papers: 3 positive, 2 negative or no effect

2 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

1 paper more in this group

See all 5 papers →

loss of function · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results

18 papers: 6 positive, 12 negative or no effect

5 papers in the same direction as this paper · 12 in the opposite direction

  1. negative
  2. negative
  3. negative

14 papers more in this group

See all 18 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.