Paper · 1980 · Biochemistry

Chloroplast biogenesis: detection of divinylprotochlorophyllide ester in higher plants

Faith C. Belanger; Constantin A. Rebeiz

8
claims
15
verbatim quotes
29
citations
2
research areas
—
Not open access

Abstract

It is shown that the protochlorophyllide ester pool of etiolated higher plants is a faithful copy of the protochlorophyllide pool. It is made up of both monovinyl- and divinylprotochlorophyllide esters. Although the two tetrapyrroles exhibited similar emission maxima, they were distinguishable by their Soret excitation maxima, which were found at 436-437 and 443-444 nm, respectively, in ether at 77K. The two pigments were partially separated on thin layers of polyethylene. They were accompanied by two unknown fluorescent compounds. It was also shown that during greening, the protochlorophyllide ester pool maintained a constant qualitative composition. This was in sharp contrast with the drastic qualitative changes undergone by the protochlorophyllide pool of etiolated tissues grown under identical conditions.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

The ester pool copied the protochlorophyllide pool in etiolated higher plants.

Taxon
higher plantsEmbryophytae0
Organ / stage
etiolatede0
Outcome
protochlorophyllide ester poole0

Not reportedDirection

Verbatim

e0

“the protochlorophyllide ester pool of etiolated higher plants is a faithful copy of the protochlorophyllide pool”

✓ located verbatim in the source · abstract, characters 17–129 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The ester pool contained both monovinyl and divinyl esters.

Taxon
higher plantsEmbryophytae1
Outcome
monovinyl- and divinylprotochlorophyllide esterse0

Not reportedDirection

Verbatim

e0

“It is made up of both monovinyl- and divinylprotochlorophyllide esters.”

✓ located verbatim in the source · abstract, characters 131–202 · show in text ↑
e1

“higher plants”

✓ located verbatim in the source · abstract, characters 65–78 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The pigments had similar emission maxima.

Taxon
higher plantsEmbryophytae1
Outcome
similar emission maximae0

Not reportedDirection

Verbatim

e0

“the two tetrapyrroles exhibited similar emission maxima”

✓ located verbatim in the source · abstract, characters 212–267 · show in text ↑
e1

“higher plants”

✓ located verbatim in the source · abstract, characters 65–78 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The pigments had distinct Soret excitation maxima at 436-437 and 443-444 nm.

Taxon
higher plantsEmbryophytae1
Outcome
Soret excitation maximae0
Value
436-437 and 443-444 nme0

Not reportedDirection

Verbatim

e0

“they were distinguishable by their Soret excitation maxima, which were found at 436-437 and 443-444 nm, respectively, in ether at 77K”

✓ located verbatim in the source · abstract, characters 269–402 · show in text ↑
e1

“higher plants”

✓ located verbatim in the source · abstract, characters 65–78 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The pigments were partly separated on polyethylene thin layers.

Taxon
higher plantsEmbryophytae1
Outcome
partially separatede0

Not reportedComparatorValue

Verbatim

e0

“The two pigments were partially separated on thin layers of polyethylene.”

✓ located verbatim in the source · abstract, characters 404–477 · show in text ↑
e1

“higher plants”

✓ located verbatim in the source · abstract, characters 65–78 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Two unknown fluorescent compounds accompanied the pigments.

Taxon
higher plantsEmbryophytae1
Outcome
two unknown fluorescent compoundse0

Not reportedDirection

Verbatim

e0

“They were accompanied by two unknown fluorescent compounds.”

✓ located verbatim in the source · abstract, characters 478–537 · show in text ↑
e1

“higher plants”

✓ located verbatim in the source · abstract, characters 65–78 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

The ester pool maintained its qualitative composition during greening.

Taxon
higher plantsEmbryophytae1
Outcome
protochlorophyllide ester poole0
Direction
maintained a constant qualitative compositione0

Verbatim

e0

“during greening, the protochlorophyllide ester pool maintained a constant qualitative composition”

✓ located verbatim in the source · abstract, characters 561–658 · show in text ↑
e1

“higher plants”

✓ located verbatim in the source · abstract, characters 65–78 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The protochlorophyllide pool changed qualitatively during greening.

Taxon
higher plantsEmbryophytae1
Outcome
protochlorophyllide poole0
Direction
drastic qualitative changese0

Verbatim

e0

“drastic qualitative changes undergone by the protochlorophyllide pool of etiolated tissues grown under identical conditions”

✓ located verbatim in the source · abstract, characters 696–819 · show in text ↑
e1

“higher plants”

✓ located verbatim in the source · abstract, characters 65–78 · show in text ↑