Abstract
The genome of Ob, a tobamovirus that overcomes the N gene-mediated hypersensitive response (HR), was cloned as a cDNA, and its nucleotide sequence was determined. The genomic organization of Ob is similar to that of other tobamoviruses, consisting of 6506 nucleotides and containing at least four open reading frames. These open reading frames encode a 126-kD polypeptide with a 183-kD readthrough product, a 30.6-kD movement protein, and an 18-kD coat protein. A bacteriophage T7 promoter sequence was fused to the full-length cDNA clone to obtain infectious RNA transcripts. These transcripts, when inoculated onto tobacco plants, induced disease symptoms indistinguishable from plants inoculated with Ob viral RNA. To determine which viral factor is responsible for the resistance-breaking character of Ob, a recombinant virus was constructed in which the movement protein gene of tobacco mosaic virus was replaced with that of Ob. Cultivar Xanthi NN tobacco plants infected with this virus responded with an HR, indicating that the Ob movement protein alone does not act to overcome the N gene-mediated response. Following mutagenesis of the infectious Ob cDNA clone with hydroxylamine, populations of transcripts from the mutagenized DNA were inoculated onto Xanthi NN tobacco, and a variant that induced the HR was identified. The mutant was analyzed and found to contain a single nucleotide change in the 126-kD gene. Recreating the mutation in the Ob cDNA clone by site-directed mutagenesis resulted in a virus that caused symptoms identical to the chemically induced mutant.
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Claims 9
Claim · model-written summary
The Ob virus genome consists of 6506 nucleotides with at least four open reading frames.
- Taxon
- tobacco
Nicotiana tabacumt0 - Interactor
- Obe1pathogen
- Outcome
- genomic organizatione1
- Value
- 6506 nucleotidese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance”
e1“The genomic organization of Ob is similar to that of other tobamoviruses, consisting of 6506 nucleotides and containing at least four open reading frames”
Claim · model-written summary
The open reading frames of Ob encode a 126-kD polypeptide.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- Obe1pathogen
- Outcome
- polypeptidee7
- Value
- 126-kDe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance”
e1“The genomic organization of Ob is similar to that of other tobamoviruses, consisting of 6506 nucleotides and containing at least four open reading frames”
e7“These open reading frames encode a 126-kD polypeptide with a 183-kD readthrough product, a 30.6-kD movement protein, and an 18-kD coat protein”
Claim · model-written summary
The open reading frames of Ob encode a 183-kD readthrough product.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- Obe1pathogen
- Outcome
- readthrough producte7
- Value
- 183-kDe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance”
e1“The genomic organization of Ob is similar to that of other tobamoviruses, consisting of 6506 nucleotides and containing at least four open reading frames”
e7“These open reading frames encode a 126-kD polypeptide with a 183-kD readthrough product, a 30.6-kD movement protein, and an 18-kD coat protein”
Claim · model-written summary
The open reading frames of Ob encode a 30.6-kD movement protein.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- Obe1pathogen
- Outcome
- movement proteine7
- Value
- 30.6-kDe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance”
e1“The genomic organization of Ob is similar to that of other tobamoviruses, consisting of 6506 nucleotides and containing at least four open reading frames”
e7“These open reading frames encode a 126-kD polypeptide with a 183-kD readthrough product, a 30.6-kD movement protein, and an 18-kD coat protein”
Claim · model-written summary
The open reading frames of Ob encode an 18-kD coat protein.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- Obe1pathogen
- Outcome
- coat proteine7
- Value
- 18-kDe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance”
e1“The genomic organization of Ob is similar to that of other tobamoviruses, consisting of 6506 nucleotides and containing at least four open reading frames”
e7“These open reading frames encode a 126-kD polypeptide with a 183-kD readthrough product, a 30.6-kD movement protein, and an 18-kD coat protein”
Claim · model-written summary
Infectious RNA transcripts of the Ob cDNA clone induced disease symptoms on tobacco indistinguishable from plants inoculated with Ob viral RNA.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- infectious RNA transcriptse3
- Interactor
- Obe2pathogen
- Outcome
- disease symptomse2
- Comparator
- plants inoculated with Ob viral RNAe2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“These transcripts, when inoculated onto tobacco plants, induced disease symptoms indistinguishable from plants inoculated with Ob viral RNA”
e3“to obtain infectious RNA transcripts”
Claim · model-written summary
A recombinant virus carrying the Ob movement protein gene did not overcome N gene-mediated resistance; tobacco responded with a hypersensitive response.
- Taxon
- Xanthi NN tobaccoe3
- Material
- Xanthi NNe3
- Treatment
- movement protein gene of tobacco mosaic virus was replaced with that of Obe4
- Interactor
- Obe3pathogen
- Outcome
- HRe3
Not reportedComparatorDirection
Verbatim
e3“Cultivar Xanthi NN tobacco plants infected with this virus responded with an HR, indicating that the Ob movement protein alone does not act to overcome the N gene-mediated response”
e4“a recombinant virus was constructed in which the movement protein gene of tobacco mosaic virus was replaced with that of Ob”
Claim · model-written summary
A hydroxylamine-induced Ob variant that induced the HR carried a single nucleotide change in the 126-kD gene.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- 126-kD genee5
- Outcome
- single nucleotide changee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance”
e5“The mutant was analyzed and found to contain a single nucleotide change in the 126-kD gene”
e6“a variant that induced the HR was identified”
Claim · model-written summary
Site-directed mutagenesis of the Ob cDNA clone produced a virus causing symptoms identical to the chemically induced mutant on tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- site-directed mutagenesise2
- Interactor
- Obe2pathogen
- Outcome
- symptomse2
- Comparator
- chemically induced mutante2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance”
e2“Recreating the mutation in the Ob cDNA clone by site-directed mutagenesis resulted in a virus that caused symptoms identical to the chemically induced mutant”
The same questions in other papers
infection · disease · Nicotiana tabacum (tobacco)Positive and negative results
10 papers in the same direction as this paper · 4 reporting no difference from an alternative
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.