Abstract
A lectin without blood group specificity was isolated from cowpeas (Vigna unguiculata (L.) Walp.). This lectin was prepared from a crude saline extract by acid precipitation and Sephadex gel filtration. Purification steps generally resulted in increased agglutination activity. Homogeneity was demonstrated by polyacrylamide gel electrophoresis, and ultracentrifugation. The native lectin had a molecular weight of approximately 55,000 as shown by gel filtration, polyacrylamide electrophoresis, and analytical ultracentrifugation. Amino acid analysis showed a very low sulfur amino acid content and relatively high content of aspartic and glutamic acids. Total neutral sugar analysis indicated less than 1% of saccharides. The lectin interacted with several saccharides.
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Claims 7
Claim · model-written summary
A cowpea lectin without blood group specificity was isolated.
- Taxon
- cowpeas
Vigna unguiculatae0 - Gene
- lectine0
- Outcome
- without blood group specificitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A lectin without blood group specificity was isolated from cowpeas (Vigna unguiculata (L.) Walp.).”
Claim · model-written summary
Purification steps generally increased lectin agglutination activity.
- Taxon
- CowpeasVigna unguiculatat0
- Outcome
- agglutination activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Purification steps generally resulted in increased agglutination activity.”
t0“Cowpeas”
Claim · model-written summary
The isolated lectin was homogeneous according to electrophoresis and ultracentrifugation.
- Taxon
- CowpeasVigna unguiculatat0
- Outcome
- Homogeneitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Homogeneity was demonstrated by polyacrylamide gel electrophoresis, and ultracentrifugation.”
t0“Cowpeas”
Claim · model-written summary
The native lectin had a molecular weight of approximately 55,000.
- Taxon
- CowpeasVigna unguiculatat0
- Gene
- lectine0
- Outcome
- molecular weighte0
- Value
- approximately 55,000e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The native lectin had a molecular weight of approximately 55,000”
t0“Cowpeas”
Claim · model-written summary
The lectin had very low sulfur amino acid content and relatively high aspartic and glutamic acid content.
- Taxon
- CowpeasVigna unguiculatat0
- Outcome
- very low sulfur amino acid content and relatively high content of aspartic and glutamic acidse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amino acid analysis showed a very low sulfur amino acid content and relatively high content of aspartic and glutamic acids.”
t0“Cowpeas”
Claim · model-written summary
The lectin had less than 1% saccharides.
- Taxon
- CowpeasVigna unguiculatat0
- Outcome
- saccharidese0
- Value
- less than 1%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Total neutral sugar analysis indicated less than 1% of saccharides.”
t0“Cowpeas”
Claim · model-written summary
The lectin interacted with several saccharides.
- Taxon
- CowpeasVigna unguiculatat0
- Gene
- lectine0
- Outcome
- interacted with several saccharidese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lectin interacted with several saccharides.”
t0“Cowpeas”