Abstract
In the present study, the endophytic bacterium Bacillus subtilis strain Em7 (GU258545.1) was evaluated as a biological control agent for Sclerotinia sclerotiorum on oilseed rape. In petri dish, strain Em7 not only strongly inhibited pathogen mycelium growth but also germination of sclerotia at concentrations between 109 and 1011 colony forming unit (CFU)·ml−1. Scanning electron microscopy and transmission electron microscopy studies revealed that in the presence of strain Em7, hyphae of S. sclerotiorum showed leakage and disintegration of hyphal cytoplasm. Furthermore, the strain Em7 showed a broad antifungal spectrum on mycelium growth of numerous important plant pathogenic fungi. Light microscopic observations revealed that strain Em7 caused morphological alterations including increased branching, swelling and collapse of cytoplasm. In the greenhouse, spray treatments of cell suspensions of strain Em7 (1×109 CFU·ml−1) reduced leaf and stem rot incidence and severity in the seedling and blossom stage. The control efficacy was higher when strain Em7 cell suspension was applied one day prior to inoculation of the pathogen than after inoculation. Three-year field trials showed that two applications of strain Em7 cell suspension at blossom stage significantly reduced disease incidence and severity by 50–70%. There was no significant difference in control efficacy among treatments with strain Em7 cell suspension and the fungicides containing carbendazim or tebuconazole (P = 0.05). Thus, our results strongly suggest that B. subtilis strain Em7 is a promising biological control agent for control of oilseed rape Sclerotinia stem rot.
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Claims 10
Claim · model-written summary
Em7 inhibited Sclerotinia mycelium growth in petri dishes.
- Taxon
- oilseed rape
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Treatment
- strain Em7e0
- Interactor
- Sclerotinia sclerotiorum
Sclerotinia sclerotiorume1pathogen - Second interactor
- Bacillus subtilis strain Em7
Bacillus subtilise1biocontrol_agent - Outcome
- pathogen mycelium growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“strain Em7 not only strongly inhibited pathogen mycelium growth but also germination of sclerotia at concentrations between 109 and 1011 colony forming unit (CFU)·ml−1.”
e1“Bacillus subtilis strain Em7 (GU258545.1) was evaluated as a biological control agent for Sclerotinia sclerotiorum on oilseed rape”
Claim · model-written summary
Em7 inhibited Sclerotinia sclerotia germination.
- Taxon
- oilseed rapeBrassica napuse1
- Treatment
- strain Em7e0
- Interactor
- Sclerotinia sclerotiorumSclerotinia sclerotiorume1pathogen
- Second interactor
- Bacillus subtilis strain Em7Bacillus subtilise1biocontrol_agent
- Outcome
- germination of sclerotiae0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strain Em7 not only strongly inhibited pathogen mycelium growth but also germination of sclerotia at concentrations between 109 and 1011 colony forming unit (CFU)·ml−1.”
e1“Bacillus subtilis strain Em7 (GU258545.1) was evaluated as a biological control agent for Sclerotinia sclerotiorum on oilseed rape”
Claim · model-written summary
Sclerotinia hyphae leaked and their cytoplasm disintegrated in the presence of Em7.
- Taxon
- oilseed rapeBrassica napuse1
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Second interactor
- strain Em7e0biocontrol_agent
- Outcome
- hyphae of S. sclerotiorum showed leakage and disintegration of hyphal cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the presence of strain Em7, hyphae of S. sclerotiorum showed leakage and disintegration of hyphal cytoplasm.”
e1“on oilseed rape”
Claim · model-written summary
Em7 inhibited mycelial growth of numerous plant pathogenic fungi.
- Taxon
- oilseed rapeBrassica napuse1
- Interactor
- plant pathogenic fungie0pathogen
- Second interactor
- strain Em7e0biocontrol_agent
- Outcome
- mycelium growth of numerous important plant pathogenic fungie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strain Em7 showed a broad antifungal spectrum on mycelium growth of numerous important plant pathogenic fungi.”
e1“on oilseed rape”
Claim · model-written summary
Em7 caused branching, swelling and cytoplasmic collapse in fungal structures.
- Taxon
- oilseed rapeBrassica napuse1
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume2pathogen
- Second interactor
- strain Em7e0biocontrol_agent
- Outcome
- morphological alterations including increased branching, swelling and collapse of cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strain Em7 caused morphological alterations including increased branching, swelling and collapse of cytoplasm.”
e1“on oilseed rape”
e2“S. sclerotiorum showed leakage”
Claim · model-written summary
Greenhouse Em7 spray reduced leaf and stem rot incidence and severity.
- Taxon
- oilseed rapeBrassica napuse1
- Organ / stage
- seedling and blossom stagee0
- Treatment
- spray treatments of cell suspensions of strain Em7 (1×109 CFU·ml−1)e0
- Interactor
- Sclerotinia sclerotiorumSclerotinia sclerotiorume1pathogen
- Second interactor
- Bacillus subtilis strain Em7Bacillus subtilise1biocontrol_agent
- Outcome
- leaf and stem rot incidence and severitye0
- Direction
- reducede0
- Setting
- greenhousee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“spray treatments of cell suspensions of strain Em7 (1×109 CFU·ml−1) reduced leaf and stem rot incidence and severity in the seedling and blossom stage.”
e1“Bacillus subtilis strain Em7 (GU258545.1) was evaluated as a biological control agent for Sclerotinia sclerotiorum on oilseed rape”
e2“In the greenhouse”
Claim · model-written summary
Applying Em7 one day before pathogen inoculation gave higher control efficacy than applying it afterward.
- Taxon
- oilseed rapeBrassica napuse1
- Treatment
- strain Em7 cell suspension was applied one day prior to inoculation of the pathogene0
- Interactor
- Sclerotinia sclerotiorumSclerotinia sclerotiorume1pathogen
- Second interactor
- Bacillus subtilis strain Em7Bacillus subtilise1biocontrol_agent
- Outcome
- control efficacye0
- Direction
- highere0
- Comparator
- after inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The control efficacy was higher when strain Em7 cell suspension was applied one day prior to inoculation of the pathogen than after inoculation.”
e1“Bacillus subtilis strain Em7 (GU258545.1) was evaluated as a biological control agent for Sclerotinia sclerotiorum on oilseed rape”
Claim · model-written summary
Two Em7 applications at blossom significantly reduced field disease incidence and severity by 50–70%.
- Taxon
- oilseed rapeBrassica napuse1
- Treatment
- two applications of strain Em7 cell suspension at blossom stagee0
- Interactor
- Sclerotinia sclerotiorumSclerotinia sclerotiorume1pathogen
- Second interactor
- Bacillus subtilis strain Em7Bacillus subtilise1biocontrol_agent
- Outcome
- disease incidence and severitye0
- Direction
- reducede0
- Value
- 50–70%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two applications of strain Em7 cell suspension at blossom stage significantly reduced disease incidence and severity by 50–70%.”
e1“Bacillus subtilis strain Em7 (GU258545.1) was evaluated as a biological control agent for Sclerotinia sclerotiorum on oilseed rape”
Claim · model-written summary
Em7 and the carbendazim or tebuconazole fungicides did not differ significantly in control efficacy.
- Taxon
- oilseed rapeBrassica napuse1
- Interactor
- Sclerotinia sclerotiorumSclerotinia sclerotiorume1pathogen
- Second interactor
- Bacillus subtilis strain Em7Bacillus subtilise1biocontrol_agent
- Outcome
- control efficacye0
- Direction
- no significant differencee0
- Comparator
- fungicides containing carbendazim or tebuconazolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no significant difference in control efficacy among treatments with strain Em7 cell suspension and the fungicides containing carbendazim or tebuconazole (P = 0.05).”
e1“Bacillus subtilis strain Em7 (GU258545.1) was evaluated as a biological control agent for Sclerotinia sclerotiorum on oilseed rape”
Claim · model-written summary
The authors propose Em7 as a biological control agent for oilseed rape Sclerotinia stem rot.
- Taxon
- oilseed rapeBrassica napuse0
- Interactor
- Sclerotinia
Sclerotiniae0pathogen - Second interactor
- B. subtilis strain Em7Bacillus subtilise0biocontrol_agent
- Outcome
- promising biological control agent for control of oilseed rape Sclerotinia stem rote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. subtilis strain Em7 is a promising biological control agent for control of oilseed rape Sclerotinia stem rot.”
The same questions in other papers
inoculation · Sclerotinia sclerotiorum · Brassica napus (oilseed rape)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- positive
- no effect
- negative
6 papers more in this group
See all 10 papers →application · Sclerotinia sclerotiorum · Brassica napus (oilseed rape)Positive and negative results
8 papers in the same direction as this paper · 7 in the opposite direction
- positive
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.