Abstract
Tomato (Lycopersicon esculantum) ASR1 (abscisic acid stress ripening protein), a small plant-specific protein whose cellular mode of action defies deduction based on its sequence or homology analyses, is one of numerous plant gene products with unknown biological roles that become over-expressed under water- and salt-stress conditions. Steady-state cellular levels of tomato ASR1 mRNA and protein are transiently increased following exposure of plants to poly(ethylene glycol), NaCl or abscisic acid. Western blot and indirect immunofluorescence analysis with anti-ASR1 antibodies demonstrated that ASR1 is present both in the cytoplasmic and nuclear subcellular compartments; approx. one-third of the total ASR1 protein could be detected in the nucleus. Nuclear ASR1 is a chromatin-bound protein, and can be extracted with 1 M NaCl, but not with 0.5% Triton X-100. ASR1, overexpressed in Escherichia coli and purified to homogeneity, possesses zinc-dependent DNA-binding activity. Competitive-binding experiments and SELEX (systematic evolution of ligands by exponential enrichment) analysis suggest that ASR1 binds at a preferred DNA sequence.
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Claims 8
Claim · model-written summary
Tomato ASR1 mRNA and protein transiently increased after poly(ethylene glycol).
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- poly(ethylene glycol)e0
- Gene
- ASR1e0
- Outcome
- ASR1 mRNA and proteine0
- Direction
- increasede0
- Qualifier
- transientlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Steady-state cellular levels of tomato ASR1 mRNA and protein are transiently increased following exposure of plants to poly(ethylene glycol), NaCl or abscisic acid.”
Claim · model-written summary
Tomato ASR1 mRNA and protein transiently increased after NaCl.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- NaCle0
- Gene
- ASR1e0
- Outcome
- ASR1 mRNA and proteine0
- Direction
- increasede0
- Qualifier
- transientlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Steady-state cellular levels of tomato ASR1 mRNA and protein are transiently increased following exposure of plants to poly(ethylene glycol), NaCl or abscisic acid.”
Claim · model-written summary
Tomato ASR1 mRNA and protein transiently increased after abscisic acid.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- abscisic acide0
- Gene
- ASR1e0
- Outcome
- ASR1 mRNA and proteine0
- Direction
- increasede0
- Qualifier
- transientlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Steady-state cellular levels of tomato ASR1 mRNA and protein are transiently increased following exposure of plants to poly(ethylene glycol), NaCl or abscisic acid.”
Claim · model-written summary
ASR1 occurs in cytoplasmic and nuclear compartments.
- Taxon
- TomatoSolanum lycopersicume1
- Gene
- ASR1e0
- Outcome
- cytoplasmic and nuclear subcellular compartmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Western blot and indirect immunofluorescence analysis with anti-ASR1 antibodies demonstrated that ASR1 is present both in the cytoplasmic and nuclear subcellular compartments; approx. one-third of the total ASR1 protein could be detected in the nucleus.”
e1“Tomato (Lycopersicon esculantum) ASR1”
Claim · model-written summary
About one-third of ASR1 protein was detected in the nucleus.
- Taxon
- TomatoSolanum lycopersicume1
- Gene
- ASR1e0
- Outcome
- ASR1 proteine0
- Value
- approx. one-thirde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Western blot and indirect immunofluorescence analysis with anti-ASR1 antibodies demonstrated that ASR1 is present both in the cytoplasmic and nuclear subcellular compartments; approx. one-third of the total ASR1 protein could be detected in the nucleus.”
e1“Tomato (Lycopersicon esculantum) ASR1”
Claim · model-written summary
Nuclear ASR1 is chromatin-bound and extractable with salt but not detergent.
- Taxon
- TomatoSolanum lycopersicume1
- Gene
- ASR1e0
- Outcome
- chromatin-bound proteine0
- Qualifier
- 1 M NaCl, but not with 0.5% Triton X-100e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclear ASR1 is a chromatin-bound protein, and can be extracted with 1 M NaCl, but not with 0.5% Triton X-100.”
e1“Tomato (Lycopersicon esculantum) ASR1”
Claim · model-written summary
Purified ASR1 has zinc-dependent DNA-binding activity.
- Taxon
- TomatoSolanum lycopersicume1
- Gene
- ASR1e0
- Outcome
- zinc-dependent DNA-binding activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ASR1, overexpressed in Escherichia coli and purified to homogeneity, possesses zinc-dependent DNA-binding activity.”
e1“Tomato (Lycopersicon esculantum) ASR1”
Claim · model-written summary
ASR1 appears to bind a preferred DNA sequence.
- Taxon
- TomatoSolanum lycopersicume1
- Gene
- ASR1e0
- Outcome
- preferred DNA sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Competitive-binding experiments and SELEX (systematic evolution of ligands by exponential enrichment) analysis suggest that ASR1 binds at a preferred DNA sequence.”
e1“Tomato (Lycopersicon esculantum) ASR1”
The same questions in other papers
sodium chloride · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
5 papers in the same direction as this paper · 6 in the opposite direction
- positive
- negative
- negative
8 papers more in this group
See all 12 papers →poly(ethylene glycol) · molecular: expression · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.