Abstract
Previous studies have shown that Azospirillum or its culture filtrates induce root hairs in wheat seedlings to branch; this phenomenon is influenced by the bacterial genome at the strain level and by the plant genome at the cultivar level. Strain Sp245 caused most branching of root hairs; between wheat cultivars frequency of branching increased in the following order: cv. Tobari, cv. Tonari, cv. BH1146, cv. Lagoa. Similarly, plant growth response to inoculation with Azospirillum, measured in mature plants, increased in the same order, suggesting that both phenomena (root hair branching and plant growth response) have a similar basis. In this study, the "branching substance" present in Azospirillum culture filtrate was found to be thermostable at 100 °C, removed by dialysis, present in the acidic nonpolar fraction of the cell-free extract, and co-chromatographed with authentic indole acetic acid on thin-layer chromatograms. Purified indole acetic acid caused branching of root hairs similar to that caused by the Azospirillum culture filtrates. Susceptibility of wheat cultivars to indole acetic acid induced branching increased in the order: cv. Tobari, cv. Tonari, cv. BH1146, cv. Lagoa. Of five strains examined, strain Sp245 produced the most indole acetic acid in culture in the presence of tryptophan. The presence of indole acetic acid was confirmed by gas chromatography – mass spectrometry.
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Claims 11
Claim · model-written summary
Sp245 caused the most wheat root-hair branching.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- Strain Sp245e0
- Outcome
- branching of root hairse0
- Direction
- moste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strain Sp245 caused most branching of root hairs”
t0“wheat root hairs”
Claim · model-written summary
Root-hair branching increased across wheat cultivars from Tobari through Lagoa.
- Taxon
- wheatTriticum aestivumt0
- Material
- cv. Tobari, cv. Tonari, cv. BH1146, cv. Lagoae0
- Outcome
- frequency of branchinge0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“between wheat cultivars frequency of branching increased in the following order: cv. Tobari, cv. Tonari, cv. BH1146, cv. Lagoa.”
t0“wheat root hairs”
Claim · model-written summary
Mature plant growth response to Azospirillum inoculation followed the same cultivar order.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- inoculation with Azospirillume0
- Outcome
- plant growth responsee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant growth response to inoculation with Azospirillum, measured in mature plants, increased in the same order”
t0“wheat root hairs”
Claim · model-written summary
The branching substance was stable at 100 °C.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- "branching substance"e0
- Qualifier
- thermostable at 100 °Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the "branching substance" present in Azospirillum culture filtrate was found to be thermostable at 100 °C”
t0“wheat root hairs”
Claim · model-written summary
The substance was removed by dialysis.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- removed by dialysise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“removed by dialysis”
t0“wheat root hairs”
Claim · model-written summary
The substance was in the acidic nonpolar fraction.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- present in the acidic nonpolar fraction of the cell-free extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“present in the acidic nonpolar fraction of the cell-free extract”
t0“wheat root hairs”
Claim · model-written summary
The substance co-chromatographed with indole acetic acid.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- co-chromatographed with authentic indole acetic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-chromatographed with authentic indole acetic acid on thin-layer chromatograms”
t0“wheat root hairs”
Claim · model-written summary
Purified indole acetic acid induced branching similar to culture filtrate.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Purified indole acetic acide0
- Outcome
- branching of root hairse0
- Direction
- similare0
- Comparator
- Azospirillum culture filtratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Purified indole acetic acid caused branching of root hairs similar to that caused by the Azospirillum culture filtrates.”
t0“wheat root hairs”
Claim · model-written summary
Cultivar susceptibility to auxin-induced branching rose from Tobari to Lagoa.
- Taxon
- wheatTriticum aestivumt0
- Material
- cv. Tobari, cv. Tonari, cv. BH1146, cv. Lagoae0
- Outcome
- Susceptibility of wheat cultivars to indole acetic acid induced branchinge0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Susceptibility of wheat cultivars to indole acetic acid induced branching increased in the order: cv. Tobari, cv. Tonari, cv. BH1146, cv. Lagoa.”
t0“wheat root hairs”
Claim · model-written summary
Sp245 produced the most indole acetic acid in culture with tryptophan.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- indole acetic acide0
- Direction
- moste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strain Sp245 produced the most indole acetic acid in culture in the presence of tryptophan.”
t0“wheat root hairs”
Claim · model-written summary
Gas chromatography–mass spectrometry confirmed indole acetic acid.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- presence of indole acetic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of indole acetic acid was confirmed by gas chromatography – mass spectrometry.”
t0“wheat root hairs”
The same questions in other papers
indole-3-acetic acid · growth · Triticum aestivum (wheat)Results differ
32 papers in the same direction as this paper · 5 in the opposite direction
- negative
- no effect
- negative
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.