Abstract
Little information is available on estimating in vitro dry matter digestibility (IVDMD) of alfalfa (Medicago sativa L.) herbage by a prepared cellulase solution (PCS) and then using these IVDMD estimates to calibrate near infrared reflectance spectroscopy (NIRS) equations. Objectives were to compare PCS digestion to that by two rumen fermentation procedures, including true in vitro digestibility (TIVD), and develop NIRS equations to estimate TIVD, neutral detergent fiber, and acid detergent fiber of alfalfa hay. Seventy-eight alfalfa samples, having a wide range in herbage quality, were analyzed for IVDMD using five different PCS procedures and two rumen fermentation procedures (true and apparent in vitro digestibility). The best NIRS calibration equation for TIVD had R 2 of 0.92 and a standard error of selection of 20.7 g kg −1 . Correlations between IVDMD and TIVD obtained by the various PCS assays ranged from 0.91 to 0.96 (P < 0.01), with regression coefficients ranging from 0.94 to 0.98. We concluded that PCS gave rapid and accurate estimates of TIVD and that NIRS could accurately estimate TIVD of a wide range of alfalfa herbage quality.Key words: Acid detergent solubles, fungal cellulase solubles, in vitro digestible dry matter, Medicago sativa L., neutral detergent solubles, alfalfa
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Claims 6
Claim · model-written summary
Correlations between IVDMD from the cellulase assays and TIVD ranged from 0.91 to 0.96.
- Taxon
- alfalfa
Medicago sativat0 - Outcome
- Correlations between IVDMD and TIVDe0
- Value
- 0.91 to 0.96e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Correlations between IVDMD and TIVD obtained by the various PCS assays ranged from 0.91 to 0.96 (P < 0.01)”
t0“QUALITY OF ALFALFA HERBAGE”
Claim · model-written summary
Prepared cellulase solution digestion gave rapid and accurate estimates of TIVD, compared with two rumen fermentation procedures.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- accurate estimates of TIVDe2
- Comparator
- two rumen fermentation procedurese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PCS gave rapid and accurate estimates of TIVD”
e3“compare PCS digestion to that by two rumen fermentation procedures”
t0“QUALITY OF ALFALFA HERBAGE”
Claim · model-written summary
The best NIRS calibration equation for TIVD had R2 of 0.92.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- calibration equation for TIVDe4
- Value
- R 2 of 0.92e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The best NIRS calibration equation for TIVD had R 2 of 0.92”
t0“QUALITY OF ALFALFA HERBAGE”
Claim · model-written summary
The best NIRS calibration equation for TIVD had a standard error of selection of 20.7 g kg −1.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- standard error of selectione5
- Value
- 20.7 g kg −1e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“a standard error of selection of 20.7 g kg −1”
t0“QUALITY OF ALFALFA HERBAGE”
Claim · model-written summary
NIRS could accurately estimate TIVD across a wide range of alfalfa herbage quality.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- accurately estimate TIVDe6
- Comparator
- TIVDe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“NIRS could accurately estimate TIVD of a wide range of alfalfa herbage quality”
t0“QUALITY OF ALFALFA HERBAGE”
Claim · model-written summary
Regression coefficients between IVDMD and TIVD from the cellulase assays ranged from 0.94 to 0.98.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- regression coefficientse1
- Value
- 0.94 to 0.98e1
- Qualifier
- between IVDMD and TIVDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Correlations between IVDMD and TIVD obtained by the various PCS assays ranged from 0.91 to 0.96 (P < 0.01)”
e1“with regression coefficients ranging from 0.94 to 0.98”
t0“QUALITY OF ALFALFA HERBAGE”