Abstract
Different combinations of chromosome N- or C-banding with in situ hybridization (ISH) or genomic in situ hybridization (GISH) were sequentially performed on metaphase chromosomes of wheat. A modified N-banding-ISH/GISH sequential procedure gave best results. Similarly, a modified C-banding - ISH/GISH procedure also gave satisfactory results. The variation of the hot acid treatment in the standard chromosome N- or C-banding procedures was the major factor affecting the resolution of the subsequent ISH and GISH. By the sequential chromosome banding - ISH/GISH analysis, multicopy DNA sequences and the breakpoints of wheat-alien translocations were directly allocated to specific chromosomes of wheat. The sequential chromosome banding- ISH/GISH technique should be widely applicable in genome mapping, especially in cytogenetic and molecular mapping of heterochromatic and euchromatic regions of plant and animal chromosomes.
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Claims 5
Claim · model-written summary
A modified N-banding-ISH/GISH sequential procedure gave the best results among the combinations tested in wheat.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- modified N-banding-ISH/GISH sequential proceduree1
- Outcome
- best resultse1
- Comparator
- Different combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different combinations of chromosome N- or C-banding with in situ hybridization (ISH) or genomic in situ hybridization (GISH) were sequentially performed on metaphase chromosomes of wheat”
e1“A modified N-banding-ISH/GISH sequential procedure gave best results”
Claim · model-written summary
A modified C-banding-ISH/GISH procedure also gave satisfactory results on wheat chromosomes.
- Taxon
- wheatTriticum aestivume0
- Treatment
- modified C-banding - ISH/GISH proceduree2
- Outcome
- satisfactory resultse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metaphase chromosomes of wheat”
e2“Similarly, a modified C-banding - ISH/GISH procedure also gave satisfactory results”
Claim · model-written summary
The variation of the hot acid treatment was the major factor affecting the resolution of the subsequent ISH and GISH in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- variation of the hot acid treatmente3
- Outcome
- resolution of the subsequent ISH and GISHe3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metaphase chromosomes of wheat”
e3“The variation of the hot acid treatment in the standard chromosome N- or C-banding procedures was the major factor affecting the resolution of the subsequent ISH and GISH”
Claim · model-written summary
Multicopy DNA sequences and the breakpoints of wheat-alien translocations were directly allocated to specific chromosomes of wheat.
- Taxon
- wheatTriticum aestivume0
- Material
- wheat-alien translocationse4
- Outcome
- allocated to specific chromosomese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metaphase chromosomes of wheat”
e4“multicopy DNA sequences and the breakpoints of wheat-alien translocations were directly allocated to specific chromosomes of wheat”
Claim · model-written summary
The sequential chromosome banding-ISH/GISH technique should be widely applicable in genome mapping.
- Taxon
- wheatTriticum aestivume0
- Outcome
- widely applicable in genome mappinge5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metaphase chromosomes of wheat”
e5“The sequential chromosome banding- ISH/GISH technique should be widely applicable in genome mapping”