Claim · model-written summary
The variation of the hot acid treatment was the major factor affecting the resolution of the subsequent ISH and GISH in wheat.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- variation of the hot acid treatmente3
- Outcome
- resolution of the subsequent ISH and GISHe3
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metaphase chromosomes of wheat”
e3“The variation of the hot acid treatment in the standard chromosome N- or C-banding procedures was the major factor affecting the resolution of the subsequent ISH and GISH”
Title
Sequential chromosome banding and in situ hybridization analysis
Jiming Jiang; Bikram S. Gill Genome 1993. doi:10.1139/g93-104
Other claims from this paper (4)
- Method / resource A modified N-banding-ISH/GISH sequential procedure gave the best results among the combinations tested in wheat.
- Observation A modified C-banding-ISH/GISH procedure also gave satisfactory results on wheat chromosomes.
- Observation Multicopy DNA sequences and the breakpoints of wheat-alien translocations were directly allocated to specific chromosomes of wheat.
- Recommendation The sequential chromosome banding-ISH/GISH technique should be widely applicable in genome mapping.