Abstract
Abstract: Wide cross and molecular cytogenetic methods were used to transfer the powdery mildew resistance gene from Thinopyron intermedium(Host) Barkworth & DR Dewey to wheat. Among the progeny of crossing common wheat (Triticum aestivum L.) Yannong 15 with Th. intermedium, a partial amphiploid E990256, with resistance to powdery mildew, was developed. It had 56 chromosomes and could form 28 bivalents in pollen mother cells at metaphase I of meiosis. Resistance verification by race 15 at the seedling stage and by mixed strains of Erysiphales gramnis DC. f. sp. tritici Em. Marchal at the adult stage showed it was immune to powdery mildew at both stages. Gene postulation via 21 isolates of E. gramnis f. sp. tritici and 29 differential hosts showed it was nearly immune to all the isolates used, and its resistance pattern was different from all the mildew resistance genes used, which indicated it probably contained a new resistance gene to powdery mildew. Biochemical verification showed it might convey different Th. intermedium chromosomes from those of the wheat- Th. intermedium partial amphiploids Zhong 1–5. Genomic in situ hybridization analysis by using St genomic DNA as the probe showed E990256 contained a recombination genome of St and E. (Managing editor: Wei WANG)
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Claims 8
Claim · model-written summary
A powdery mildew resistant wheat amphiploid E990256 was developed.
- Taxon
- wheat
Triticum aestivume1 - Material
- E990256e0
- Outcome
- resistance to powdery mildewe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a partial amphiploid E990256, with resistance to powdery mildew, was developed”
e1“to wheat.”
Claim · model-written summary
E990256 had 56 chromosomes.
- Taxon
- wheatTriticum aestivume2
- Material
- E990256e1
- Outcome
- chromosomese0
- Value
- 56 chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It had 56 chromosomes and could form 28 bivalents in pollen mother cells at metaphase I of meiosis”
e1“a partial amphiploid E990256”
e2“to wheat.”
Claim · model-written summary
E990256 formed 28 bivalents at metaphase I.
- Taxon
- wheatTriticum aestivume2
- Material
- E990256e1
- Outcome
- bivalentse0
- Value
- 28 bivalentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“could form 28 bivalents in pollen mother cells at metaphase I of meiosis”
e1“a partial amphiploid E990256”
e2“to wheat.”
Claim · model-written summary
E990256 was immune to powdery mildew at seedling and adult stages.
- Taxon
- wheatTriticum aestivume3
- Material
- E990256e2
- Interactor
- Erysiphales gramnis DC. f. sp. tritici Em. Marchale1pathogen
- Outcome
- immune to powdery mildewe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was immune to powdery mildew at both stages”
e1“seedling stage and by mixed strains of Erysiphales gramnis DC. f. sp. tritici Em. Marchal at the adult stage”
e2“a partial amphiploid E990256”
e3“to wheat.”
Claim · model-written summary
E990256 was nearly immune to all tested isolates and differed from known resistance patterns.
- Taxon
- wheatTriticum aestivume2
- Material
- E990256e1
- Outcome
- nearly immune to all the isolates usede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was nearly immune to all the isolates used, and its resistance pattern was different from all the mildew resistance genes used”
e1“a partial amphiploid E990256”
e2“to wheat.”
Claim · model-written summary
The resistance pattern suggested E990256 probably contains a new resistance gene.
- Taxon
- wheatTriticum aestivume2
- Material
- E990256e1
- Gene
- resistance genee0
- Outcome
- new resistance gene to powdery mildewe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it probably contained a new resistance gene to powdery mildew”
e1“a partial amphiploid E990256”
e2“to wheat.”
Claim · model-written summary
Biochemical verification suggested different Th. intermedium chromosomes than Zhong 1–5.
- Taxon
- wheatTriticum aestivume0
- Material
- E990256e1
- Outcome
- different Th. intermedium chromosomese0
- Comparator
- Zhong 1–5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it might convey different Th. intermedium chromosomes from those of the wheat- Th. intermedium partial amphiploids Zhong 1–5”
e1“a partial amphiploid E990256”
Claim · model-written summary
E990256 contained a recombination genome of St and E.
- Taxon
- wheatTriticum aestivume1
- Material
- E990256e0
- Outcome
- recombination genome of St and Ee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“E990256 contained a recombination genome of St and E”
e1“to wheat.”