Paper · 1974 · Physiologia Plantarum

In vitro Plant Formation from Stem Explants of Rape (Brassica napus cv. Zephyr)

K. K. KARTHA; O. L. GAMBORG; F. CONSTABEL

8
claims
17
verbatim quotes
67
citations
1
research areas
—
Not open access

Abstract

Abstract Internodal segments from 6‐weeks‐old rape plants ( Brassica napus L. cv. Zephyr) were induced to differentiate in vitro producing shoots or shoots and roots on synthetic nutrient medium under controlled conditions. Benzyladenine (BA) alone (5 × 10 −6 M) induced multiple shoot formation on all stem explants. Roots were induced on shoots when recultured on nutrient medium supplemented with auxins such as naphthalene‐acetic acid (NAA) or indoleacetic acid (1AA) or when planted in vermiculite. Complete plant formation was obtained when NAA (2 × 1 −6 , 5 × 10 −6 and 10 −5 M) was employed in conjunction with BA at 5 × 10 −6 M. At higher concentrations (10 −5 M ) NAA retards the shoot development while 1AA suppresses it totally. Lower levels of auxins along with the cytokinin did not retard or inhibit shoot differentiation.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BA alone induced multiple shoots on every stem explant.

Taxon
rape plantsBrassica napuse0
Material
cv. Zephyre0
Treatment
Benzyladenine (BA) alone (5 × 10 −6 M)e1
Outcome
multiple shoot formatione1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“Benzyladenine (BA) alone (5 × 10 −6 M) induced multiple shoot formation on all stem explants.”

✓ located verbatim in the source · abstract, characters 224–317 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA induced roots on recultured shoots.

Taxon
rape plantsBrassica napuse0
Treatment
naphthalene‐acetic acid (NAA)e1
Outcome
Roots were induced on shootse1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“Roots were induced on shoots when recultured on nutrient medium supplemented with auxins such as naphthalene‐acetic acid (NAA) or indoleacetic acid (1AA) or when planted in vermiculite.”

✓ located verbatim in the source · abstract, characters 318–503 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IAA induced roots on recultured shoots.

Taxon
rape plantsBrassica napuse0
Treatment
indoleacetic acid (1AA)e1
Outcome
Roots were induced on shootse1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“Roots were induced on shoots when recultured on nutrient medium supplemented with auxins such as naphthalene‐acetic acid (NAA) or indoleacetic acid (1AA) or when planted in vermiculite.”

✓ located verbatim in the source · abstract, characters 318–503 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Vermiculite planting induced roots on shoots.

Taxon
rape plantsBrassica napuse0
Treatment
planted in vermiculitee1
Outcome
Roots were induced on shootse1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“Roots were induced on shoots when recultured on nutrient medium supplemented with auxins such as naphthalene‐acetic acid (NAA) or indoleacetic acid (1AA) or when planted in vermiculite.”

✓ located verbatim in the source · abstract, characters 318–503 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA with BA yielded complete plant formation.

Taxon
rape plantsBrassica napuse0
Treatment
NAA (2 × 1 −6 , 5 × 10 −6 and 10 −5 M) was employed in conjunction with BA at 5 × 10 −6 Me1
Outcome
Complete plant formatione1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“Complete plant formation was obtained when NAA (2 × 1 −6 , 5 × 10 −6 and 10 −5 M) was employed in conjunction with BA at 5 × 10 −6 M.”

✓ located verbatim in the source · abstract, characters 504–637 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Higher NAA concentration retarded shoot development.

Taxon
rape plantsBrassica napuse0
Treatment
higher concentrations (10 −5 M ) NAAe1
Outcome
shoot developmente1
Direction
retardse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“At higher concentrations (10 −5 M ) NAA retards the shoot development”

✓ located verbatim in the source · abstract, characters 638–707 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

IAA completely suppressed shoot development at higher concentration.

Taxon
rape plantsBrassica napuse0
Treatment
1AAe1
Outcome
shoot developmente2
Direction
suppressese1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“At higher concentrations (10 −5 M ) NAA retards the shoot development while 1AA suppresses it totally.”

✓ located verbatim in the source · abstract, characters 638–740 · show in text ↑
e2

“shoot development”

✓ located verbatim in the source · abstract, characters 690–707 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Lower auxin levels with cytokinin did not inhibit shoot differentiation.

Taxon
rape plantsBrassica napuse0
Treatment
Lower levels of auxins along with the cytokinine1
Outcome
shoot differentiatione1
Direction
did not retard or inhibite1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rape plants ( Brassica napus L. cv. Zephyr)”

✓ located verbatim in the source · abstract, characters 46–89 · show in text ↑
e1

“Lower levels of auxins along with the cytokinin did not retard or inhibit shoot differentiation.”

✓ located verbatim in the source · abstract, characters 741–837 · show in text ↑

1-naphthaleneacetic acid · growth · Brassica napus (oilseed rape)Positive and negative results

22 papers: 19 positive, 3 negative or no effect

2 papers in the same direction as this paper · 19 in the opposite direction

  1. positive
  2. positive
  3. positive

18 papers more in this group

See all 22 papers →

N-benzyladenine · growth · Brassica napus (oilseed rape)Positive and negative results

30 papers: 27 positive, 3 negative or no effect

26 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. negative

26 papers more in this group

See all 30 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.