Abstract
The objective of the study was to develop an efficient method for shoot regeneration of canola (Brassica napus L.) and to compare the regeneration capacity of different explants on MS medium with several combinations of plant growth regulators. The experiments showed that the morphogenetical potential of canola depends on genotype, primary explant, hormonal structure and concentration of nutrient medium. Cotyledons possessed higher regeneration ability in comparison to hypocotyls and roots. The best regeneration capability was exhibited by the cultivar 'Quantum'. Its frequency with cotyledonary explants reached 68.8% on all used media. Addition of 3 mg/l ABA in nutrient medium considerably increased the regeneration frequency. The highest shoot regeneration (100%), however, took place when cotyledonary explants were cultivated on medium, containing 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABA. Precultivation of explants on callus induction medium did not affect the shoot regeneration frequency. Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium.
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Claims 7
Claim · model-written summary
Canola morphogenetic potential depended on genotype, explant and medium composition.
- Taxon
- canola
.jpg?width=60)
Brassica napus var. napuse0 - Treatment
- hormonal structure and concentration of nutrient mediume0
- Outcome
- morphogenetical potentiale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the morphogenetical potential of canola depends on genotype, primary explant, hormonal structure and concentration of nutrient medium”
Claim · model-written summary
Cotyledons regenerated more readily than hypocotyls and roots.
- Taxon
- canolaBrassica napus var. napust0
- Organ / stage
- Cotyledonse0
- Outcome
- regeneration abilitye0
- Direction
- highere0
- Comparator
- hypocotyls and rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledons possessed higher regeneration ability in comparison to hypocotyls and roots”
t0“canola”
Claim · model-written summary
Quantum had the best regeneration capability, reaching 68.8% from cotyledon explants.
- Taxon
- canolaBrassica napus var. napust0
- Material
- Quantume0
- Organ / stage
- cotyledonary explantse0
- Outcome
- regeneration capabilitye0
- Direction
- beste0
- Value
- 68.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best regeneration capability was exhibited by the cultivar 'Quantum'. Its frequency with cotyledonary explants reached 68.8% on all used media”
t0“canola”
Claim · model-written summary
Adding 3 mg/l ABA increased regeneration frequency.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- Addition of 3 mg/l ABAe0
- Outcome
- regeneration frequencye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 3 mg/l ABA in nutrient medium considerably increased the regeneration frequency”
t0“canola”
Claim · model-written summary
A medium with NAA, BAP and ABA produced 100% shoot regeneration in cotyledon explants.
- Taxon
- canolaBrassica napus var. napust0
- Organ / stage
- cotyledonary explantse0
- Treatment
- medium, containing 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABAe0
- Outcome
- shoot regeneratione0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot regeneration (100%), however, took place when cotyledonary explants were cultivated on medium, containing 1.0 mg/l NAA, 8.0 mg/l BAP and 3.0 mg/l ABA”
t0“canola”
Claim · model-written summary
Precultivation on callus induction medium did not affect shoot regeneration frequency.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- Precultivation of explants on callus induction mediume0
- Outcome
- shoot regeneration frequencye0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Precultivation of explants on callus induction medium did not affect the shoot regeneration frequency”
t0“canola”
Claim · model-written summary
Increasing NAA, BAP or ABA promoted regenerant vitrification.
- Taxon
- canolaBrassica napus var. napust0
- Treatment
- increasing the auxin NAA or cytokinin BAP, and ABAe0
- Outcome
- Vitrification of regenerantse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitrification of regenerants was promoted by increasing the auxin NAA or cytokinin BAP, and ABA in the nutrient medium”
t0“canola”
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