Abstract
국내 육성 추파 품종인 '영산' 유채의 자엽 부착 잎자루 부위를 이용하여 재분화와 형질전환의 효율 증진에 영향을 주는 요인을 살펴보았다. 줄기 형성율은 NAA 0.5 mg/L와 Kinetin 2~4mg/L가 혼용 첨가된 배지에서 61~71%로 가장 양호하였다. 또한 질산은(Silver nitrate) 5~9 mg/L 첨가는 재분화에 필수적이었고, $GA_3$ 0.01 mg/L 첨가는 재분화에 긍정적인 영향을 주는 것으로 관찰되었다. 아그로박테리움의 Strain 종류, 공동배양 시간, 그리고 형질전환체 초기 선발시 항생제 농도 조절(저${\rightarrow}$고농도)에 따른 '영산' 유채의 형질전환 효율을 살펴본 결과, 아그로박테리움의 Strain 선정이 가장 중요한 요인으로 조사되었다. 특히, EHA105 succinamopine strain을 사용하였을 때 형질전환 효율이 26.8%로 가장 효과적이었다. Bar 유전자와 GUS 유전자가 내재된 형질전환체는 제초제 '바스타' 살포시 생존하고, X-Gluc으로 염색되었다. 그리고 Southern 분석을 통해 후대로 유전자가 안정적으로 유전됨을 확인하였다. To improve genetic transformation of Brassica napus winter cultivar 'Youngsan', factors influencing shoot regeneration and transformation from cotyledonary petioles were investigated. Shoot induction was enhanced in the combination of 0.5 mg/L NAA and 2~4 mg/L kinetin. Silver nitrate was essential for successful shoot regeneration, ranging from 5 to 9 mg/L. The addition of $GA_3$ promoted plant regeneration. Among the tested Agrobacterium strains, co-cultivation times, and antibiotic selection regimes, choice of appropriate Agrobacterium strain was the most critical factor for efficient transformation of B. napus cv. 'Youngsan'. The EHA105 succinamopine strain was the most efficient and the maximum transformation efficiency was 26.8%. Transgenic shoots were selected on 10 mg/L phosphinothricin (PPT) containing media. The transgenic plants expressing bar and gus genes were resistant for commercial herbicide "Basta" and stained with X-Gluc. Southern blot hybridization indicated that the presence of one to three gus gene copies per genome and inheritance of the gus gene into the $T_1$ generation.
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Claims 9
Claim · model-written summary
NAA with kinetin enhanced shoot induction.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- 0.5 mg/L NAA and 2~4 mg/L kinetine0
- Outcome
- Shoot inductione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Shoot induction was enhanced in the combination of 0.5 mg/L NAA and 2~4 mg/L kinetin.”
t0“Brassica napus”
Claim · model-written summary
Silver nitrate was essential for shoot regeneration.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Silver nitratee0
- Outcome
- shoot regeneratione0
- Value
- 5 to 9 mg/Le0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silver nitrate was essential for successful shoot regeneration, ranging from 5 to 9 mg/L.”
t0“Brassica napus”
Claim · model-written summary
GA3 addition promoted plant regeneration.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- $GA_3$e0
- Outcome
- plant regeneratione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of $GA_3$ promoted plant regeneration.”
t0“Brassica napus”
Claim · model-written summary
Agrobacterium strain choice was the most critical transformation factor.
- Taxon
- B. napusBrassica napuse0
- Material
- Youngsane0
- Interactor
- Agrobacterium
.jpg?width=60)
Agrobacteriume0other - Outcome
- efficient transformatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“choice of appropriate Agrobacterium strain was the most critical factor for efficient transformation of B. napus cv. 'Youngsan'.”
Claim · model-written summary
EHA105 gave maximum transformation efficiency of 26.8%.
- Taxon
- Brassica napusBrassica napust0
- Interactor
- EHA105 succinamopine straine0other
- Outcome
- transformation efficiencye0
- Direction
- maximume0
- Value
- 26.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The EHA105 succinamopine strain was the most efficient and the maximum transformation efficiency was 26.8%.”
t0“Brassica napus”
Claim · model-written summary
Transgenic bar and gus plants resisted Basta herbicide.
- Taxon
- Brassica napusBrassica napust0
- Gene
- bar and gus genese0
- Outcome
- resistant for commercial herbicide "Basta"e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants expressing bar and gus genes were resistant for commercial herbicide "Basta"”
t0“Brassica napus”
Claim · model-written summary
Transgenic bar and gus plants stained with X-Gluc.
- Taxon
- Brassica napusBrassica napust0
- Gene
- bar and gus genese0
- Outcome
- stained with X-Gluce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants expressing bar and gus genes were resistant for commercial herbicide "Basta" and stained with X-Gluc.”
t0“Brassica napus”
Claim · model-written summary
Transgenic plants contained one to three gus copies per genome.
- Taxon
- Brassica napusBrassica napust0
- Gene
- gus genee0
- Outcome
- gus gene copies per genomee0
- Value
- one to threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of one to three gus gene copies per genome”
t0“Brassica napus”
Claim · model-written summary
The gus gene was inherited into the T1 generation.
- Taxon
- Brassica napusBrassica napust0
- Gene
- gus genee0
- Outcome
- inheritance of the gus gene into the $T_1$ generatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inheritance of the gus gene into the $T_1$ generation”
t0“Brassica napus”
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