Abstract
The model dicotyledon Arabidopsis thaliana has a characteristic small sub-family of phytochrome-interacting bHLH (basic Helix-Loop-Helix) factors, which are collectively designated the PIL (or PIF) (PHYTOCHROME INTERACTING FACTOR-LIKE) family proteins. In this study, we identified and characterized a set of highly homologous members (designated OsPIL11 to OsPIL16) in the model monocotyledon rice (Oryza sativa). Some of them (OsPIL11, OsPIL12, and OsPIL13) showed the ability to interact with the putative OsPRR1 (PSEUDO-RESPONSE REGULATOR 1) clock component, as far as the results of yeast two-hybrid assays were concerned. It was found that the expression of OsPIL13 is under the control of circadian rhythms (clock), while the expression of OsPIL15 is negatively regulated by light upon the onset to light exposure of etiolated seedlings. When the rice genes (OsPIL11 to OsPIL15) were over-expressed in A. thaliana, the resulting transgenic seedlings displayed anomalous morphologies with very long hypocotyls during early photomorphogenesis. These results suggest the view that the identified OsPILs are functional counterparts (or orthologs) of AtPILs, which are known to play important roles in red light-mediated (phyA and/or phyB-dependent) signal transduction pathways at immediate positions downstream of the photoreceptor in A. thaliana.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Six homologous rice PIL proteins were identified and characterized.
- Taxon
- rice (Oryza sativa)
Oryza sativae0 - Gene
- OsPIL11 to OsPIL16e0
- Outcome
- highly homologous memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified and characterized a set of highly homologous members (designated OsPIL11 to OsPIL16) in the model monocotyledon rice (Oryza sativa)”
Claim · model-written summary
OsPIL11, OsPIL12 and OsPIL13 interacted with OsPRR1 in yeast two-hybrid assays.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Gene
- OsPIL11, OsPIL12, and OsPIL13e0
- Outcome
- ability to interact with the putative OsPRR1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsPIL11, OsPIL12, and OsPIL13) showed the ability to interact with the putative OsPRR1 (PSEUDO-RESPONSE REGULATOR 1) clock component, as far as the results of yeast two-hybrid assays were concerned”
e1“rice (Oryza sativa)”
Claim · model-written summary
OsPIL13 expression was controlled by circadian rhythms.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Gene
- OsPIL13e0
- Outcome
- expression of OsPIL13e0
- Qualifier
- circadian rhythms (clock)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of OsPIL13 is under the control of circadian rhythms (clock)”
e1“rice (Oryza sativa)”
Claim · model-written summary
Light negatively regulated OsPIL15 expression at light onset in etiolated seedlings.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Organ / stage
- etiolated seedlingse0
- Treatment
- lighte0
- Gene
- OsPIL15e0
- Outcome
- expression of OsPIL15e0
- Direction
- negatively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of OsPIL15 is negatively regulated by light upon the onset to light exposure of etiolated seedlings”
e1“rice (Oryza sativa)”
Claim · model-written summary
Overexpressing rice OsPIL11 to OsPIL15 in Arabidopsis caused anomalously long hypocotyls during early photomorphogenesis.
- Taxon
- A. thaliana
Arabidopsis thalianae0 - Organ / stage
- seedlingse0
- Treatment
- over-expressede0
- Gene
- OsPIL11 to OsPIL15e0
- Outcome
- very long hypocotylse0
- Direction
- very longe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the rice genes (OsPIL11 to OsPIL15) were over-expressed in A. thaliana, the resulting transgenic seedlings displayed anomalous morphologies with very long hypocotyls during early photomorphogenesis”
Claim · model-written summary
The authors interpret the identified OsPIL proteins as functional counterparts of AtPILs.
- Taxon
- rice (Oryza sativa)Oryza sativae1
- Gene
- OsPILse0
- Outcome
- functional counterparts (or orthologs) of AtPILse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the identified OsPILs are functional counterparts (or orthologs) of AtPILs”
e1“rice (Oryza sativa)”
The same questions in other papers
light · molecular: expression · Oryza sativa (rice)Positive and negative results
11 papers in the same direction as this paper · 20 in the opposite direction
- positive
- positive
- positive
28 papers more in this group
See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.