Abstract
Histochemical tests were made wich the resting as well as dividing nuclei in plants with FEULGEN's nucleai-reaction method for the test of thymusnucleic acid, MILLON'S, the xanthoproteic and the biuret reaction method for Proteins, and CHRISTELLER'S, CIACCIO's and other methods of testing for lipoids. The solution analysis method and staining according to PAPPENHEIM-UNNA'S method were also used as supplemen-tary means of testing the results obtained with the methods named above. The results we obtained with these methods are as follows:1) The nuclear reticulum and chromosomes show reactions of thymusnucleic acid, lipoids and proteins.2) In the chromosomes the thymusnucleic acid reaction is found exclusively in the spiral portion.3) The chromosomes are far more easily dissolved by lipoid solvents and nucleoprotein solvents than the resting nucleus is (Tables 1 and 2).4) Of the two morphological components f of chromosomes, the spiral portion and matrix, the former is more resistant to the action of lipoid solvents than the latter.5) There is some evidente which suggests that certain material changes may take place in the nuclear components during mitosis.6) The nucleus of Spirogyra sp. shows no thymusnucleic acid reaction but a protein reaction, forming a striking contrast to the other plants investigated.7) The karyolymph show no positive result in any of the tests for thymusnucleic acid, Lipoids, and proteins, but the result obtained with the precipitation method suggests that there may be lipoid in the karyolymph.8) The nucleolus is shown to contain lipoids, but no thymusnucleic acid.9) The spindle fibers and the phragmoplast are shown to consist mainly of proteins and Lipoids in a mixed or a combined form.In concluding, the authors wish to express their sintere thanks to Prof. Y. KUWADA for his kind guidance throughout the investigation.
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Claims 9
Claim · model-written summary
Plant nuclear reticulum and chromosomes reacted for thymusnucleic acid, lipoids and proteins.
Verbatim
e0“The nuclear reticulum and chromosomes show reactions of thymusnucleic acid, lipoids and proteins.”
t0“Plant Nuclei”
Claim · model-written summary
In plant chromosomes, the thymusnucleic acid reaction occurred only in the spiral portion.
Not reportedTaxon
Verbatim
e0“In the chromosomes the thymusnucleic acid reaction is found exclusively in the spiral portion.”
Claim · model-written summary
Plant chromosomes dissolved more readily in lipoid and nucleoprotein solvents than resting nuclei.
- Treatment
- lipoid solvents and nucleoprotein solventse0
- Outcome
- more easily dissolvede0
- Comparator
- resting nucleuse0
Not reportedTaxon
Verbatim
e0“The chromosomes are far more easily dissolved by lipoid solvents and nucleoprotein solvents than the resting nucleus is”
Claim · model-written summary
The chromosome spiral portion was more resistant to lipoid solvents than the matrix.
- Organ / stage
- spiral portione0
- Outcome
- more resistant to the action of lipoid solventse0
- Comparator
- matrixe0
Not reportedTaxon
Verbatim
e0“the spiral portion and matrix, the former is more resistant to the action of lipoid solvents than the latter”
Claim · model-written summary
The authors found suggestive evidence of material changes in nuclear components during mitosis.
Not reportedTaxon
Verbatim
e0“There is some evidente which suggests that certain material changes may take place in the nuclear components during mitosis.”
Claim · model-written summary
Spirogyra nuclei lacked a thymusnucleic acid reaction but showed a protein reaction.
SpirogyraOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleus of Spirogyra sp. shows no thymusnucleic acid reaction but a protein reaction”
Claim · model-written summary
Karyolymph tested negative for thymusnucleic acid, lipoids and proteins, although precipitation suggested possible lipoid.
- Organ / stage
- karyolymphe0
- Outcome
- no positive result in any of the tests for thymusnucleic acid, Lipoids, and proteinse0
- Qualifier
- there may be lipoid in the karyolymphe0
Not reportedTaxon
Verbatim
e0“The karyolymph show no positive result in any of the tests for thymusnucleic acid, Lipoids, and proteins, but the result obtained with the precipitation method suggests that there may be lipoid in the karyolymph.”
Claim · model-written summary
The nucleolus contained lipoids but no thymusnucleic acid.
Not reportedTaxon
Verbatim
e0“The nucleolus is shown to contain lipoids, but no thymusnucleic acid.”
Claim · model-written summary
Spindle fibers and phragmoplast consisted mainly of proteins and lipoids in mixed or combined form.
- Organ / stage
- spindle fibers and the phragmoplaste0
- Outcome
- consist mainly of proteins and Lipoids in a mixed or a combined forme0
Not reportedTaxon
Verbatim
e0“The spindle fibers and the phragmoplast are shown to consist mainly of proteins and Lipoids in a mixed or a combined form.”