Abstract
Optimum conditions for the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) to a water-insoluble red formazan have been determined for suspension tissue cultures of Acer saccharum and Haplopappus gracilus. The standard incubation mixture is 0.8% TTC dissolved in a 2:1 solution of 0.05 M sodium phosphate buffer:Acer growth medium, pH 7.5. After the cells are incubated for 18–20 h in the dark at room temperature, the red formazan is extracted with 95% ethanol, and the absorbance is read at 485 nm. The use of the TTC procedure as a viability assay is supported by studies with Haplopappus tissue cultures, in which viability can be assayed by both plating in agar and the TTC procedure. These studies show that there is a quantitative correspondence between the decrease in the plating assay and in the TTC assay when these cells are subjected to freezing stress and plasmolysis stress.
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Claims 2
Claim · model-written summary
The TTC assay decrease corresponded quantitatively to the plating assay decrease in Haplopappus cultures under freezing and plasmolysis stress.
- Taxon
- Haplopappus
Haplopappuse3 - Treatment
- freezing stress and plasmolysis stresse1
- Outcome
- TTC assaye1
- Direction
- quantitative correspondencee1
- Comparator
- plating assaye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These studies show that there is a quantitative correspondence between the decrease in the plating assay and in the TTC assay when these cells are subjected to freezing stress and plasmolysis stress”
e3“The use of the TTC procedure as a viability assay is supported by studies with Haplopappus tissue cultures”
Claim · model-written summary
The standard TTC incubation mixture for Acer saccharum cultures contained 0.8% TTC.
- Taxon
- Acer saccharum
Acer saccharume0 - Outcome
- reduction of 2,3,5-triphenyltetrazolium chloride (TTC)e0
- Value
- 0.8%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimum conditions for the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) to a water-insoluble red formazan have been determined for suspension tissue cultures of Acer saccharum and Haplopappus gracilus”
e2“The standard incubation mixture is 0.8% TTC dissolved in a 2:1 solution of 0.05 M sodium phosphate buffer:Acer growth medium, pH 7.5”