Abstract
BACKGROUND: Hydroponics is a plant growth system that provides a more precise control of growth media composition. Several hydroponic systems have been reported for Arabidopsis and other model plants. The ease of system set up, cost of the growth system and flexibility to characterize and harvest plant material are features continually improved in new hydroponic system reported. RESULTS: We developed a hydroponic culture system for Arabidopsis and other model plants. This low cost, proficient, and novel system is based on recyclable and sterilizable plastic containers, which are readily available from local suppliers. Our system allows a large-scale manipulation of seedlings. It adapts to different growing treatments and has an extended growth window until adult plants are established. The novel seed-holder also facilitates the transfer and harvest of seedlings. Here we report the use of our hydroponic system to analyze transcriptomic responses of Arabidopsis to nutriment availability and plant/pathogen interactions. CONCLUSIONS: The efficiency and functionality of our proposed hydroponic system is demonstrated in nutrient deficiency and pathogenesis experiments. Hydroponically grown Arabidopsis seedlings under long-time inorganic phosphate (Pi) deficiency showed typical changes in root architecture and high expression of marker genes involved in signaling and Pi recycling. Genome-wide transcriptional analysis of gene expression of Arabidopsis roots depleted of Pi by short time periods indicates that genes related to general stress are up-regulated before those specific to Pi signaling and metabolism. Our hydroponic system also proved useful for conducting pathogenesis essays, revealing early transcriptional activation of pathogenesis-related genes.
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Claims 7
Claim · model-written summary
The hydroponic system supports large-scale seedling manipulation.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- large-scale manipulation of seedlingse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our system allows a large-scale manipulation of seedlings”
t0“Arabidopsis”
Claim · model-written summary
The system accommodates different treatments and growth through adulthood.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- extended growth windowe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It adapts to different growing treatments and has an extended growth window until adult plants are established”
t0“Arabidopsis”
Claim · model-written summary
The seed-holder facilitates seedling transfer and harvest.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- transfer and harvest of seedlingse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel seed-holder also facilitates the transfer and harvest of seedlings”
t0“Arabidopsis”
Claim · model-written summary
Long phosphate deficiency changed root architecture.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- roote0
- Treatment
- long-time inorganic phosphate (Pi) deficiencye0
- Outcome
- changes in root architecturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydroponically grown Arabidopsis seedlings under long-time inorganic phosphate (Pi) deficiency showed typical changes in root architecture”
Claim · model-written summary
Long phosphate deficiency raised expression of Pi signalling and recycling markers.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- expression of marker genes involved in signaling and Pi recyclinge0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high expression of marker genes involved in signaling and Pi recycling”
t0“Arabidopsis”
Claim · model-written summary
Short Pi depletion upregulated general-stress genes before Pi-specific genes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- genes related to general stresse0
- Outcome
- genes related to general stresse0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes related to general stress are up-regulated before those specific to Pi signaling and metabolism”
t0“Arabidopsis”
Claim · model-written summary
Pathogenesis assays revealed early activation of pathogenesis-related genes.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- early transcriptional activation of pathogenesis-related genese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pathogenesis essays, revealing early transcriptional activation of pathogenesis-related genes”
t0“Arabidopsis”