Abstract
A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and designated as TaHIR2. The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) were 31.05 kD and 5.18, respectively. Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein. Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2. Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells. Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage. These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust.
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Claims 8
Claim · model-written summary
A wheat hypersensitive-reaction gene was identified and named TaHIR2.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaHIR2e0
- Outcome
- novel gene induced during hypersensitive reactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and designated as TaHIR2.”
Claim · model-written summary
TaHIR2 was predicted to encode a 284-amino acid protein of 31.05 kD and pI 5.18.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHIR2e0
- Outcome
- 284-amino acid proteine0
- Value
- 31.05 kDe0
- Qualifier
- 5.18e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) were 31.05 kD and 5.18, respectively.”
t0“wheat”
Claim · model-written summary
TaHIR2 protein sequence contained an SPFH domain and prohibitin homologue.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHIR2e0
- Outcome
- stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein.”
t0“wheat”
Claim · model-written summary
TaHIR2 was highly homologous to HvHIR2.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHIR2e0
- Outcome
- highly homologous to HvHIR2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2.”
t0“wheat”
Claim · model-written summary
TaHIR2 fusion protein was located in onion epidermal cells in a transient expression assay.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- onion epidermal cellse0
- Gene
- TaHIR2e0
- Outcome
- located in the onion epidermal cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells.”
t0“wheat”
Claim · model-written summary
TaHIR2 transcripts accumulated significantly in adult wheat leaves, peaking 18 h after stripe rust inoculation.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- adult wheat leavese0
- Interactor
- stripe ruste0pathogen
- Gene
- TaHIR2e0
- Outcome
- TaHIR2 transcriptse0
- Direction
- accumulatede0
- Value
- 18 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe rust”
Claim · model-written summary
TaHIR2 was slightly up-regulated in the compatible reaction at the seedling stage.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- seedling stagee0
- Gene
- TaHIR2e1
- Outcome
- up-regulatione0
- Direction
- up-regulatione0
- Qualifier
- compatible reactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“slightly up-regulation could also be observed in the compatible reaction at the seedling stage”
t0“wheat”
e1“TaHIR2 transcripts”
Claim · model-written summary
The authors suggest TaHIR2 may play a role in wheat defense against stripe rust.
- Taxon
- wheatTriticum aestivume0
- Interactor
- stripe ruste0pathogen
- Gene
- TaHIR2e0
- Outcome
- wheat defense against stripe ruste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust.”