Paper · 2012 · Plant Signaling & Behavior

Analysis of differential expression of Mediator subunit genes in Arabidopsis

Richa Pasrija; Jitendra K. Thakur

8
claims
16
verbatim quotes
34
citations
1
research areas
✓
Open access

Abstract

Mediator is a conserved eukaryotic multiprotein complex required by RNA polymerase II for transcription of its target genes. Till date, there is no report explaining the signals that affect the overall concentration of individual Med subunits. In this report, we have analyzed the effect of different phytohormones and stresses on the transcript level of Med genes in Arabidopsis. Hormones like auxin and JA, and cold stress did not show significant effect. ABA moderately increased the transcript abundance of more than 70% of AtMed genes analyzed in this study. However, there was noticeable change in the transcript level of several AtMed genes in response to BR. Stresses like high light, dark and salt also caused significant change in the transcript abundance of many AtMed genes. These data reveal that different environmental cues can affect stoichiometric concentration of Med subunits by affecting the transcription of their respective genes. This may, in turn, affect the overall arrangement of functional Mediator complex. This also suggests that some subunits may have some specific functions to play in response different signals.

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Claims 8

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Auxin did not significantly affect Med gene transcript levels.

Taxon
ArabidopsisArabidopsise1
Treatment
auxine0
Gene
Med genese1
Outcome
transcript levele1
Direction
did not show significant effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Hormones like auxin and JA, and cold stress did not show significant effect”

✓ located verbatim in the source · abstract, characters 381–456 · show in text ↑
e1

“transcript level of Med genes in Arabidopsis”

✓ located verbatim in the source · abstract, characters 335–379 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

JA did not significantly affect Med gene transcript levels.

Taxon
ArabidopsisArabidopsise1
Treatment
JAe0
Gene
Med genese1
Outcome
transcript levele1
Direction
did not show significant effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Hormones like auxin and JA, and cold stress did not show significant effect”

✓ located verbatim in the source · abstract, characters 381–456 · show in text ↑
e1

“transcript level of Med genes in Arabidopsis”

✓ located verbatim in the source · abstract, characters 335–379 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Cold stress did not significantly affect Med gene transcript levels.

Taxon
ArabidopsisArabidopsise1
Treatment
cold stresse0
Gene
Med genese1
Outcome
transcript levele1
Direction
did not show significant effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“cold stress did not show significant effect”

✓ located verbatim in the source · abstract, characters 413–456 · show in text ↑
e1

“transcript level of Med genes in Arabidopsis”

✓ located verbatim in the source · abstract, characters 335–379 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ABA moderately increased transcript abundance in over 70% of analyzed AtMed genes.

Taxon
ArabidopsisArabidopsist0
Treatment
ABAe0
Gene
AtMed genese0
Outcome
transcript abundancee0
Direction
increasede0
Value
more than 70%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA moderately increased the transcript abundance of more than 70% of AtMed genes analyzed in this study”

✓ located verbatim in the source · abstract, characters 458–562 · show in text ↑
t0

“Arabidopsis”

✓ located verbatim in the source · title, characters 65–76 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

BR caused a noticeable change in several AtMed gene transcript levels.

Taxon
ArabidopsisArabidopsist0
Treatment
BRe0
Gene
AtMed genese0
Outcome
transcript levele0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“noticeable change in the transcript level of several AtMed genes in response to BR”

✓ located verbatim in the source · abstract, characters 583–665 · show in text ↑
t0

“Arabidopsis”

✓ located verbatim in the source · title, characters 65–76 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

High light significantly changed transcript abundance of many AtMed genes.

Taxon
ArabidopsisArabidopsist0
Treatment
high lighte0
Gene
AtMed genese0
Outcome
transcript abundancee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“high light, dark and salt also caused significant change in the transcript abundance of many AtMed genes”

✓ located verbatim in the source · abstract, characters 681–785 · show in text ↑
t0

“Arabidopsis”

✓ located verbatim in the source · title, characters 65–76 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Darkness significantly changed transcript abundance of many AtMed genes.

Taxon
ArabidopsisArabidopsist0
Treatment
darke0
Gene
AtMed genese0
Outcome
transcript abundancee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“high light, dark and salt also caused significant change in the transcript abundance of many AtMed genes”

✓ located verbatim in the source · abstract, characters 681–785 · show in text ↑
t0

“Arabidopsis”

✓ located verbatim in the source · title, characters 65–76 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Salt significantly changed transcript abundance of many AtMed genes.

Taxon
ArabidopsisArabidopsist0
Treatment
salte0
Gene
AtMed genese0
Outcome
transcript abundancee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis
Arabidopsis · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“high light, dark and salt also caused significant change in the transcript abundance of many AtMed genes”

✓ located verbatim in the source · abstract, characters 681–785 · show in text ↑
t0

“Arabidopsis”

✓ located verbatim in the source · title, characters 65–76 · show in text ↑

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75 papers: 46 positive, 29 negative or no effect

28 papers in the same direction as this paper · 46 in the opposite direction

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.