Abstract
BACKGROUND: Gibberellins are well known for their growth control function in flower, fruit and seed development, and as such, exogenous gibberellic acid (GA) application plays an important role in viticulture. Unfortunately, the mechanism by which GA3 acts in the regulation of these complicated developmental processes in grape remains unclear. RESULTS: In the present study, we demonstrated that application of GA3 to 'Kyoho' grapevine inflorescences at pre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion. In an attempt to obtain a deeper understanding of the molecular mechanisms driving these responses to GA3 treatment, we performed large-scale transcriptome sequencing of grape flowers following GA3 treatment using Illumina sequencing technology. Global expression profiles of GA3-treated and untreated grape flowers were compared and a large number of GA3-responsive genes were identified. Gene ontology (GO) term classification and biochemical pathway analyses indicated that GA3 treatment caused changes in the levels of transcripts involved in cellular processes, reproduction, hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS). These findings suggest that GA3-induced morphological alterations may be related to the control of hormone biosynthesis and signaling, regulation of transcription factors, alteration of secondary metabolites, and the stability of redox homeostasis. CONCLUSIONS: Taken together, this comprehensive inflorescence transcriptome data set provides novel insight into the response of grape flowers to GA3 treatment, and also provides possible candidate genes or markers that could be used to guide future efforts in this field.
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Claims 6
Claim · model-written summary
GA3 promoted flower opening at pre-bloom.
- Material
- Kyohoe0
- Organ / stage
- inflorescencese0
- Treatment
- application of GA3e0
- Outcome
- flower openinge0
- Direction
- promotede0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of GA3 to 'Kyoho' grapevine inflorescences at pre-bloom promoted flower opening”
Claim · model-written summary
GA3 induced fruit coloring.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of GA3 to 'Kyoho' grapevine inflorescences at pre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion”
Claim · model-written summary
GA3 induced seed abortion.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of GA3 to 'Kyoho' grapevine inflorescences at pre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion”
Claim · model-written summary
Many GA3-responsive genes were identified in grape flowers.
Verbatim
e0“a large number of GA3-responsive genes were identified”
t0“grapevine”
Claim · model-written summary
GA3 changed transcripts in cellular, reproductive, hormone, secondary metabolism, and ROS processes.
- Treatment
- GA3 treatmente0
- Outcome
- levels of transcriptse0
- Direction
- changese0
- Qualifier
- cellular processes, reproduction, hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA3 treatment caused changes in the levels of transcripts involved in cellular processes, reproduction, hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS)”
t0“grapevine”
Claim · model-written summary
The grape inflorescence transcriptome data set provides candidate genes or markers.
Not reportedComparatorValue
Verbatim
e0“comprehensive inflorescence transcriptome data set provides novel insight into the response of grape flowers to GA3 treatment, and also provides possible candidate genes or markers”
The same questions in other papers
Gibberellic acid · molecular: expression · VitisPositive and negative results
6 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- positive
- negative
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.