Abstract
Despite its ubiquitous presence, the role of glyoxalase I has not been well investigated in plants. In order to find out its physiological functions, we have cloned and characterized a cDNA from Brassica juncea encoding glyoxalase I (Gly I) and made transgenic tobacco plants harbouring Gly I in both sense and antisense orientation. The transgenic nature of the plants was confirmed by Southern blotting, and the estimated number of genes inserted ranged from one to six. The transcript and protein levels of glyoxalase I were also monitored in transgenic plants. The expression of glyoxalase I in B. juncea was upregulated in response to salt, water and heavy metal stresses. In response to a high concentration of salt, the transcript level averaged threefold higher in 72 h, and an increase in the protein was also seen by immunoblotting. The transgenic plants over-expressing glyoxalase I showed significant tolerance to methylglyoxal and high salt, as tested in detached leaf disc senescence assay. A comparison of plants expressing high and low levels of glyoxalase I showed that the tolerance to different salt concentrations was correlated with the degree of glyoxalase I expression. Our results suggest an important role of glyoxalase I in conferring tolerance to plants under stress conditions.
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Claims 7
Claim · model-written summary
Transgenic tobacco contained one to six inserted genes.
- Taxon
- tobacco
Nicotiana tabacumt0 - Outcome
- number of genes insertede0
- Value
- one to sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the estimated number of genes inserted ranged from one to six”
t0“tobacco”
Claim · model-written summary
Glyoxalase I expression in Brassica juncea was upregulated by salt, water and heavy metal stresses.
- Taxon
- B. juncea
Brassica junceae0 - Treatment
- salt, water and heavy metal stressese0
- Gene
- glyoxalase Ie0
- Outcome
- expression of glyoxalase Ie0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of glyoxalase I in B. juncea was upregulated in response to salt, water and heavy metal stresses”
Claim · model-written summary
High salt raised glyoxalase I transcript levels about threefold in 72 hours.
- Taxon
- Brassica junceaBrassica junceat0
- Treatment
- a high concentration of salte0
- Gene
- glyoxalase Ie1
- Outcome
- transcript levele0
- Direction
- highere0
- Value
- threefolde0
- Qualifier
- in 72 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In response to a high concentration of salt, the transcript level averaged threefold higher in 72 h”
t0“Brassica juncea”
e1“The expression of glyoxalase I in B. juncea was upregulated in response to salt, water and heavy metal stresses”
Claim · model-written summary
High salt also increased glyoxalase I protein abundance.
- Taxon
- Brassica junceaBrassica junceat0
- Treatment
- high concentration of salte1
- Gene
- Glyoxalase It0
- Outcome
- proteine0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in the protein was also seen by immunoblotting”
t0“Glyoxalase I fromBrassica juncea”
e1“a high concentration of salt”
Claim · model-written summary
Glyoxalase I overexpression significantly improved tobacco tolerance to methylglyoxal.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- over-expressing glyoxalase Ie0
- Gene
- glyoxalase Ie0
- Outcome
- tolerance to methylglyoxale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants over-expressing glyoxalase I showed significant tolerance to methylglyoxal and high salt”
t0“tobacco”
Claim · model-written summary
Glyoxalase I overexpression significantly improved tobacco tolerance to high salt.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- over-expressing glyoxalase Ie0
- Gene
- glyoxalase Ie0
- Outcome
- tolerance to methylglyoxal and high salte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants over-expressing glyoxalase I showed significant tolerance to methylglyoxal and high salt”
t0“tobacco”
Claim · model-written summary
Salt tolerance correlated with glyoxalase I expression across transgenic plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- glyoxalase Ie0
- Outcome
- tolerance to different salt concentrationse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the tolerance to different salt concentrations was correlated with the degree of glyoxalase I expression”
t0“tobacco”
The same questions in other papers
salinity · quality: protein · Brassica juncea (Indian mustard)Positive and negative results
2 in the opposite direction
- negative
- negative
heavy metal · molecular: expression · Brassica juncea (Indian mustard)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
salinity · molecular: expression · Brassica juncea (Indian mustard)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.