Abstract
The present study was aimed to isolate bioactive metabolites produced by a fungal endophyte from Helianthus annuus, Capsicum annuum, and Cucumis sativus and to assess their role in seed germination. Culture filtrate of the endophyte HA-3B from H. annuus was significantly inhibitory towards the germination and growth of lettuce seeds. HA-3B was identified as Cladosporium cladosporioides LWL5 through molecular techniques. Different concentrations (100, 500 and 1000 ppm) of the ethyl acetate extract obtained from the culture inhibited the lettuce seed germination. The extract was subjected to column chromatography and a bioassay-guided isolation method, which yielded compounds 1, 2 and an oily fraction. The oily fraction, subjected to fractionation and spectroscopic techniques, resulted in the identification of 31 different constituents. Compounds 1 and 2 were identified and characterized through MS and NMR spectroscopic techniques as benzoic acid. The bioassay results showed that this compound significantly inhibited the growth and germination of lettuce seeds. In conclusion, assessing the role of endophytes harboring essential crop plants can help us to develop potentially eco-friendly herbicides.
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Claims 6
Claim · model-written summary
HA-3B culture filtrate significantly inhibited lettuce germination and growth.
- Taxon
- lettuce
Lactuca sativae0 - Treatment
- Culture filtrate of the endophyte HA-3Be0
- Interactor
- endophyte HA-3Be0other
- Outcome
- germination and growth of lettuce seedse0
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culture filtrate of the endophyte HA-3B from H. annuus was significantly inhibitory towards the germination and growth of lettuce seeds”
Claim · model-written summary
HA-3B was identified as Cladosporium cladosporioides LWL5.
- Taxon
- Cladosporium cladosporioides LWL5
Cladosporium cladosporioidese0 - Outcome
- identified as Cladosporium cladosporioides LWL5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HA-3B was identified as Cladosporium cladosporioides LWL5 through molecular techniques”
Claim · model-written summary
Ethyl acetate extract at 100, 500 and 1000 ppm inhibited lettuce germination.
- Taxon
- lettuceLactuca sativae0
- Treatment
- 100, 500 and 1000 ppme0
- Outcome
- lettuce seed germinatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different concentrations (100, 500 and 1000 ppm) of the ethyl acetate extract obtained from the culture inhibited the lettuce seed germination”
Claim · model-written summary
Fractionation of the oily fraction identified 31 constituents.
- Taxon
- Cladosporium cladosporioides LWL5Cladosporium cladosporioidest0
- Outcome
- identification of 31 different constituentse0
- Value
- 31 different constituentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The oily fraction, subjected to fractionation and spectroscopic techniques, resulted in the identification of 31 different constituents”
t0“Cladosporium cladosporioides LWL5”
Claim · model-written summary
Compounds 1 and 2 were identified as benzoic acid.
- Taxon
- Cladosporium cladosporioides LWL5Cladosporium cladosporioidest0
- Outcome
- identified and characterized through MS and NMR spectroscopic techniques as benzoic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compounds 1 and 2 were identified and characterized through MS and NMR spectroscopic techniques as benzoic acid”
t0“Cladosporium cladosporioides LWL5”
Claim · model-written summary
Benzoic acid significantly inhibited lettuce growth and germination.
- Taxon
- lettuceLactuca sativae0
- Treatment
- this compounde0
- Outcome
- growth and germination of lettuce seedse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this compound significantly inhibited the growth and germination of lettuce seeds”
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See all 111 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.