Abstract
Competence for somatic embryogenesis of eight cassava cultivars T200, AR9-18, MTAI16, CR25-4, CM523-7, BRA1183, MCOL2261 and SM707-17 was compared to model cultivar TMS60444 in induction media containing 12 mg/l picloram or 8 mg/l 2,4-dichloro phenoxyacetic acid (2,4-D), using axillary buds (AB) and immature leaf lobes (ILL) as explants. There were significant differences (p < 0.01) among the cassava genotypes for ability to form somatic embryos (SE). In general, AB as the explant and picloram-containing medium had the highest frequency of SE for TMS60444, T200, MTAI16, CR25-4 and CM523-7 while AR9-18 had greater efficiency using ILL on picloram. Cultivars BRA1183, MCOL2261 and SM707-17 produced insignificant amounts of SE; however, loose callus and friable embryogenic callus (FEC) appeared to be the predominant tissue type observed. Five out of the eight cultivars studied showed capability of producing SE although their efficiency for somatic embryogenesis was not as high as the model cultivar TMS60444.
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Claims 11
Claim · model-written summary
Cassava genotypes differed significantly in their ability to form somatic embryos.
- Taxon
- cassava
Manihot esculentae0 - Material
- cassava genotypese0
- Outcome
- ability to form somatic embryos (SE)e0
- Value
- p < 0.01e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were significant differences (p < 0.01) among the cassava genotypes for ability to form somatic embryos (SE)”
Claim · model-written summary
Axillary buds on picloram medium gave the highest somatic embryo frequency in TMS60444.
- Taxon
- cassavaManihot esculentae1
- Material
- TMS60444e0
- Organ / stage
- ABe0
- Treatment
- picloram-containing mediume0
- Outcome
- frequency of SEe0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AB as the explant and picloram-containing medium had the highest frequency of SE for TMS60444, T200, MTAI16, CR25-4 and CM523-7”
e1“cassava cultivars”
Claim · model-written summary
Axillary buds on picloram medium gave the highest somatic embryo frequency in T200.
- Taxon
- cassavaManihot esculentae1
- Material
- T200e0
- Organ / stage
- ABe0
- Treatment
- picloram-containing mediume0
- Outcome
- frequency of SEe0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AB as the explant and picloram-containing medium had the highest frequency of SE for TMS60444, T200, MTAI16, CR25-4 and CM523-7”
e1“cassava cultivars”
Claim · model-written summary
Axillary buds on picloram medium gave the highest somatic embryo frequency in MTAI16.
- Taxon
- cassavaManihot esculentae1
- Material
- MTAI16e0
- Organ / stage
- ABe0
- Treatment
- picloram-containing mediume0
- Outcome
- frequency of SEe0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AB as the explant and picloram-containing medium had the highest frequency of SE for TMS60444, T200, MTAI16, CR25-4 and CM523-7”
e1“cassava cultivars”
Claim · model-written summary
Axillary buds on picloram medium gave the highest somatic embryo frequency in CR25-4.
- Taxon
- cassavaManihot esculentae1
- Material
- CR25-4e0
- Organ / stage
- ABe0
- Treatment
- picloram-containing mediume0
- Outcome
- frequency of SEe0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AB as the explant and picloram-containing medium had the highest frequency of SE for TMS60444, T200, MTAI16, CR25-4 and CM523-7”
e1“cassava cultivars”
Claim · model-written summary
Axillary buds on picloram medium gave the highest somatic embryo frequency in CM523-7.
- Taxon
- cassavaManihot esculentae1
- Material
- CM523-7e0
- Organ / stage
- ABe0
- Treatment
- picloram-containing mediume0
- Outcome
- frequency of SEe0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AB as the explant and picloram-containing medium had the highest frequency of SE for TMS60444, T200, MTAI16, CR25-4 and CM523-7”
e1“cassava cultivars”
Claim · model-written summary
AR9-18 had greater somatic embryogenesis efficiency from immature leaf lobes on picloram.
- Taxon
- cassavaManihot esculentae1
- Material
- AR9-18e0
- Organ / stage
- ILLe0
- Treatment
- piclorame0
- Outcome
- efficiencye0
- Direction
- greatere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AR9-18 had greater efficiency using ILL on picloram”
e1“cassava cultivars”
Claim · model-written summary
BRA1183 produced insignificant somatic embryos, with loose and friable embryogenic callus predominating.
- Taxon
- cassavaManihot esculentae1
- Material
- BRA1183e0
- Outcome
- insignificant amounts of SEe0
- Qualifier
- loose callus and friable embryogenic callus (FEC)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivars BRA1183, MCOL2261 and SM707-17 produced insignificant amounts of SE; however, loose callus and friable embryogenic callus (FEC) appeared to be the predominant tissue type observed”
e1“cassava cultivars”
Claim · model-written summary
MCOL2261 produced insignificant somatic embryos, with loose and friable embryogenic callus predominating.
- Taxon
- cassavaManihot esculentae1
- Material
- MCOL2261e0
- Outcome
- insignificant amounts of SEe0
- Qualifier
- loose callus and friable embryogenic callus (FEC)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivars BRA1183, MCOL2261 and SM707-17 produced insignificant amounts of SE; however, loose callus and friable embryogenic callus (FEC) appeared to be the predominant tissue type observed”
e1“cassava cultivars”
Claim · model-written summary
SM707-17 produced insignificant somatic embryos, with loose and friable embryogenic callus predominating.
- Taxon
- cassavaManihot esculentae1
- Material
- SM707-17e0
- Outcome
- insignificant amounts of SEe0
- Qualifier
- loose callus and friable embryogenic callus (FEC)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivars BRA1183, MCOL2261 and SM707-17 produced insignificant amounts of SE; however, loose callus and friable embryogenic callus (FEC) appeared to be the predominant tissue type observed”
e1“cassava cultivars”
Claim · model-written summary
Five of eight tested cassava cultivars could produce somatic embryos, but less efficiently than TMS60444.
- Taxon
- cassavaManihot esculentae1
- Outcome
- capability of producing SEe0
- Direction
- not as highe0
- Value
- Five out of the eight cultivarse0
- Comparator
- TMS60444e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five out of the eight cultivars studied showed capability of producing SE although their efficiency for somatic embryogenesis was not as high as the model cultivar TMS60444”
e1“cassava cultivars”