Abstract
To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.
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Claims 8
Claim · model-written summary
Forty bacterial mutants had altered virulence phenotypes in susceptible grapefruit.
- Taxon
- grapefruit
_Pompelino_Grapefruit_002.jpg?width=60)
Citrus ×paradisie0 - Interactor
- Xanthomonas citri subsp. citri 29-1Xanthomonas citri pv. citrie1pathogen
- Outcome
- mutants with altered virulence phenotypese0
- Value
- Forty mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Pompelino_Grapefruit_002.jpg?width=500)
Verbatim
e0“mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified”
e1“Xanthomonas citri subsp. citri 29-1”
Claim · model-written summary
Nine mutants lost citrus canker induction completely.
- Taxon
- grapefruitCitrus ×paradisie1
- Interactor
- Xanthomonas citri subsp. citriXanthomonas citri pv. citrit0pathogen
- Outcome
- citrus canker inductione0
- Direction
- complete losse0
- Value
- Ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nine of the mutants showed a complete loss of citrus canker induction”
e1“susceptible grapefruit”
t0“Xanthomonas citri subsp. citri”
Claim · model-written summary
Thirty-one mutants produced attenuated canker symptoms.
- Taxon
- grapefruitCitrus ×paradisie1
- Interactor
- Xanthomonas citri subsp. citriXanthomonas citri pv. citrit0pathogen
- Outcome
- canker symptomse0
- Direction
- attenuatede0
- Value
- 31 mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the other 31 mutants resulted in attenuated canker symptoms”
e1“susceptible grapefruit”
t0“Xanthomonas citri subsp. citri”
Claim · model-written summary
Each altered virulence mutant carried one Tn5 copy.
- Taxon
- grapefruitCitrus ×paradisie1
- Interactor
- Xanthomonas citri subsp. citriXanthomonas citri pv. citrit0pathogen
- Outcome
- single copy of Tn5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“each of the mutants carried a single copy of Tn5”
e1“susceptible grapefruit”
t0“Xanthomonas citri subsp. citri”
Claim · model-written summary
Plasmid rescue identified 18 different ORFs in the bacterial genome.
- Taxon
- grapefruitCitrus ×paradisie1
- Interactor
- Xanthomonas citri subsp. citriXanthomonas citri pv. citrit0pathogen
- Outcome
- different ORFse0
- Value
- 18 different ORFse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“18 different ORFs were identified in the genome sequence”
e1“susceptible grapefruit”
t0“Xanthomonas citri subsp. citri”
Claim · model-written summary
Complementation confirmed seven ORFs newly associated with bacterial virulence.
- Taxon
- grapefruitCitrus ×paradisie1
- Interactor
- X. citri subsp. citriXanthomonas citri pv. citrie0pathogen
- Outcome
- confirmed by complementation analysise0
- Value
- sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis”
e1“susceptible grapefruit”
Claim · model-written summary
The seven genes were upregulated when bacteria grew in citrus plants.
- Interactor
- Xanthomonas citri subsp. citriXanthomonas citri pv. citrit0pathogen
- Outcome
- seven genes were upregulatede0
- Direction
- upregulatede0
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seven genes were upregulated when the bacteria were grown in citrus plants”
t0“Xanthomonas citri subsp. citri”
Claim · model-written summary
The authors suggested that expression of the seven genes was essential for canker development.
- Interactor
- Xanthomonas citri subsp. citriXanthomonas citri pv. citrit0pathogen
- Outcome
- canker developmente0
- Qualifier
- expression of these genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of these genes was essential for canker development”
e1“citrus plants”
t0“Xanthomonas citri subsp. citri”