Abstract
Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37?C, did not produce acid from ?-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan?s medium and did not produce blue pigmented indigoidine on GYCA medium nor ?fried egg? colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
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Claims 3
Claim · model-written summary
Soft rot was observed on broccoli in commercial fields in western Serbia.
- Taxon
- broccoli
Brassica oleracea var. italicae0 - Outcome
- Soft rot symptomse0
- Setting
- western part of Serbiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia.”
Claim · model-written summary
Bacteria isolated from diseased broccoli were identified as Pectobacterium carotovorum subsp. carotovorum.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Interactor
- Pectobacterium carotovorum subsp. carotovorum
Pectobacterium carotovorum subsp. carotovorume0pathogen - Outcome
- identified as Pectobacterium carotovorum subsp. carotovorume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods.”
t0“broccoli soft rot”
Claim · model-written summary
The isolated strains caused discoloration and soft rot in inoculated broccoli heads.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Organ / stage
- head tissue fragmentse0
- Treatment
- inoculatede0
- Interactor
- all strainse0pathogen
- Outcome
- tissue discoloration and soft rot developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.”
The same questions in other papers
inoculation · disease · Brassica oleracea (wild cabbage)Positive and negative results
17 papers in the same direction as this paper · 13 in the opposite direction
- positive
- positive
- positive
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.