Abstract
The phytases (EC 3.1.3.26) and acid phosphatases (EC 3.1.3.2) of rice bran were purified. Four acid phosphatases were purified from rice bran: F1 and F2 had phytase activity, but F3 and F4 did not. The optimum pH of F1 and F2 for phytic acid were 4.4 and 4.6, respectively, and those of F1, F2, F3, and F4 for p-nitrophenyl phosphate were 5.1, 5.2, 5.4, and 6.0, respectively. Their molecular weights were estimated to be about 59-70K by SDS polyacrylamide gel electrophoresis, velocity density gradient centrifugation, and gel filtration on Ultrogel. The isoelectric points of F1, F2, F3, and F4 were 5.1, 5.1, 5.6, and 5.9, respectively. F1, F2, F3, and F4 had a violet color and F2 showed an absorption maximum peak at 560 nm. They had broad substrate specificities.
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Claims 11
Claim · model-written summary
Four acid phosphatases were purified from rice bran.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- brane0
- Outcome
- Four acid phosphatases were purifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four acid phosphatases were purified from rice bran”
Claim · model-written summary
F1 and F2 had phytase activity.
- Taxon
- riceOryza sativat0
- Gene
- F1 and F2e0
- Outcome
- phytase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F1 and F2 had phytase activity”
t0“rice bran”
Claim · model-written summary
F3 and F4 lacked phytase activity.
- Taxon
- riceOryza sativat0
- Gene
- F3 and F4e0
- Outcome
- phytase activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but F3 and F4 did not”
e1“F1 and F2 had phytase activity”
t0“rice bran”
Claim · model-written summary
F1 had optimum pH 4.4 for phytic acid.
- Taxon
- riceOryza sativat0
- Gene
- F1e0
- Outcome
- optimum pHe0
- Value
- 4.4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum pH of F1 and F2 for phytic acid were 4.4 and 4.6, respectively”
t0“rice bran”
Claim · model-written summary
F2 had optimum pH 4.6 for phytic acid.
- Taxon
- riceOryza sativat0
- Gene
- F2e0
- Outcome
- optimum pHe0
- Value
- 4.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum pH of F1 and F2 for phytic acid were 4.4 and 4.6, respectively”
t0“rice bran”
Claim · model-written summary
F1, F2, F3, and F4 had p-nitrophenyl phosphate optimum pH values 5.1, 5.2, 5.4, and 6.0 respectively.
- Taxon
- riceOryza sativat0
- Gene
- F1, F2, F3, and F4e0
- Outcome
- p-nitrophenyl phosphatee0
- Value
- 5.1, 5.2, 5.4, and 6.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those of F1, F2, F3, and F4 for p-nitrophenyl phosphate were 5.1, 5.2, 5.4, and 6.0, respectively”
t0“rice bran”
Claim · model-written summary
The enzyme molecular weights were about 59-70K.
- Taxon
- riceOryza sativat0
- Outcome
- molecular weightse0
- Value
- about 59-70Ke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their molecular weights were estimated to be about 59-70K”
t0“rice bran”
Claim · model-written summary
The F1, F2, F3, and F4 isoelectric points were 5.1, 5.1, 5.6, and 5.9 respectively.
- Taxon
- riceOryza sativat0
- Gene
- F1, F2, F3, and F4e0
- Outcome
- isoelectric pointse0
- Value
- 5.1, 5.1, 5.6, and 5.9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The isoelectric points of F1, F2, F3, and F4 were 5.1, 5.1, 5.6, and 5.9, respectively”
t0“rice bran”
Claim · model-written summary
F1 through F4 were violet.
- Taxon
- riceOryza sativat0
- Gene
- F1, F2, F3, and F4e0
- Outcome
- violet colore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F1, F2, F3, and F4 had a violet color”
t0“rice bran”
Claim · model-written summary
F2 showed an absorption maximum at 560 nm.
- Taxon
- riceOryza sativat0
- Gene
- F2e0
- Outcome
- absorption maximum peake0
- Value
- 560 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F2 showed an absorption maximum peak at 560 nm”
t0“rice bran”
Claim · model-written summary
The phosphatases had broad substrate specificities.
- Taxon
- riceOryza sativat0
- Outcome
- broad substrate specificitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They had broad substrate specificities.”
t0“acid phosphatases with or without phytase activity from rice bran”