Abstract
The use of bacterial antibiotic resistance markers in transgenic plants raises concerns about horizontal gene transfer to soil bacteria. We report here that kanamycin resistance in Arabidopsis thaliana can be achieved by silencing an endogenous gene encoding a putative chloroplast transporter, which presumably imports kanamycin into chloroplasts to interfere with ribosomal RNA. Homologs of the transporter exist in other plant species, suggesting this strategy may be generally useful for selecting transformed plant cells.
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Claims 2
Claim · model-written summary
Silencing a putative chloroplast transporter gene achieved kanamycin resistance in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- silencing an endogenous gene encoding a putative chloroplast transportere0
- Gene
- an endogenous gene encoding a putative chloroplast transportere0
- Outcome
- kanamycin resistancee0
- Direction
- can be achievede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kanamycin resistance in Arabidopsis thaliana can be achieved by silencing an endogenous gene encoding a putative chloroplast transporter”
Claim · model-written summary
Homologs of the transporter occur in other plant species.
Verbatim
e0“Homologs of the transporter exist in other plant species”
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