Abstract
A severe leaf curl disease has been observed since 1999 in potato (Solanum tuberosum) crops in northern India. The affected plants were severely stunted with apical leaf curl and crinkled leaves and a conspicuous mosaic. Garg et al. (2001) showed that, using polyclonal antibodies to Indian cassava mosaic virus in immunospecific electron microscopy, a begomovirus was associated with this disease. This report describes that the leaf curl disease in potato is caused by a strain of Tomato leaf curl New Delhi virus (ToLCNDV). The begomovirus from a severely affected potato plant was sap-transmitted to Nicotiana benthamiana seedlings in which it induced severe leaf distortion and stunting 15 days post-inoculation. Meristem-derived plantlets of potato cv. Kufri Anand inoculated with sap from infected N. benthamiana leaves developed yellow mottling, mosaic and leaf curl symptoms. Double-stranded viral replicative DNAs were purified from infected potato and N. benthamiana plants and cloned at HindIII, BamHI, XbaI and PstI sites in the vector pUC18. The clones at the XbaI and PstI sites had a unit genome length insert (∼2·7 kb) which hybridized with the viral replicative forms from the infected plants. The nucleotide sequence of the XbaI and PstI clones corresponded to the DNA A (accession no. AY286316) and B (accession no. AY158080) components of the begomovirus causing the disease. Comparison of the complete nucleotide sequence of DNA A revealed that it has 93–95% identity with that of ToLCNDV isolates and < 75% identity with other Tomato leaf curl virus isolates and Potato yellow mosaic virus. A whitefly-transmitted begomovirus of potato has not been reported previously in India, probably as potato crops on the north Indian plains have been cultivated during winter months when whitefly populations are low. However, in recent years, potato crops have been planted earlier and this has resulted in a sufficient build-up of whitefly populations that might have led to the emergence of a new disease in potato. This is the first observation of a begomovirus causing a severe disease of potato in India. The nucleotide sequence data indicate that the cause is a virus closely related to ToLCNDV which, unlike the strain infecting potato, is not sap-transmissible and is not known to infect potato naturally. Therefore, it is likely that a new strain of ToLCNDV has emerged that infects a new host, potato.
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Claims 11
Claim · model-written summary
Leaf curl disease in potato is caused by a strain of Tomato leaf curl New Delhi virus (ToLCNDV).
- Taxon
- potato
Solanum tuberosumt0 - Interactor
- Tomato leaf curl New Delhi virusTomato leaf curl New Delhi viruse0pathogen
- Outcome
- leaf curl diseasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“disease of potato in northern India”
e0“This report describes that the leaf curl disease in potato is caused by a strain of Tomato leaf curl New Delhi virus (ToLCNDV)”
Claim · model-written summary
The begomovirus from a severely affected potato plant, sap-transmitted to Nicotiana benthamiana seedlings, induced severe leaf distortion and stunting 15 days after inoculation.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- sap-transmittede1
- Interactor
- begomovirus
Begomoviruse1pathogen - Outcome
- severe leaf distortion and stuntinge1
- Qualifier
- 15 days post-inoculatione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The begomovirus from a severely affected potato plant was sap-transmitted to Nicotiana benthamiana seedlings in which it induced severe leaf distortion and stunting 15 days post-inoculation”
Claim · model-written summary
Meristem-derived plantlets of potato cv. Kufri Anand inoculated with sap from infected N. benthamiana leaves developed yellow mottling, mosaic and leaf curl symptoms.
- Taxon
- potatoSolanum tuberosume2
- Material
- Kufri Anande2
- Organ / stage
- Meristem-derived plantletse2
- Treatment
- inoculated with sap from infected N. benthamiana leavese2
- Outcome
- yellow mottling, mosaic and leaf curl symptomse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Meristem-derived plantlets of potato cv. Kufri Anand inoculated with sap from infected N. benthamiana leaves developed yellow mottling, mosaic and leaf curl symptoms”
Claim · model-written summary
Viral replicative DNA clones at the XbaI and PstI sites carried a unit genome length insert of about 2.7 kb that hybridized with the viral replicative forms from infected plants.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- unit genome length inserte3
- Value
- ∼2·7 kbe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“disease of potato in northern India”
e3“The clones at the XbaI and PstI sites had a unit genome length insert (∼2·7 kb) which hybridized with the viral replicative forms from the infected plants”
Claim · model-written summary
The nucleotide sequence of the XbaI and PstI clones corresponded to DNA A and B components of the begomovirus causing the disease.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- begomovirusBegomoviruse4pathogen
- Outcome
- nucleotide sequencee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“disease of potato in northern India”
e4“The nucleotide sequence of the XbaI and PstI clones corresponded to the DNA A (accession no. AY286316) and B (accession no. AY158080) components of the begomovirus causing the disease”
Claim · model-written summary
DNA A has 93–95% nucleotide identity with ToLCNDV isolates.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- ToLCNDVe5pathogen
- Outcome
- complete nucleotide sequence of DNA Ae5
- Value
- 93–95%e5
- Comparator
- ToLCNDV isolatese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“disease of potato in northern India”
e5“Comparison of the complete nucleotide sequence of DNA A revealed that it has 93–95% identity with that of ToLCNDV isolates”
Claim · model-written summary
DNA A has less than 75% nucleotide identity with other Tomato leaf curl virus isolates and Potato yellow mosaic virus.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- complete nucleotide sequence of DNA Ae6
- Value
- < 75%e6
- Comparator
- other Tomato leaf curl virus isolates and Potato yellow mosaic viruse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“disease of potato in northern India”
e6“Comparison of the complete nucleotide sequence of DNA A revealed that it has 93–95% identity with that of ToLCNDV isolates and < 75% identity with other Tomato leaf curl virus isolates and Potato yellow mosaic virus”
Claim · model-written summary
This is the first observation of a begomovirus causing a severe disease of potato in India.
- Taxon
- potatoSolanum tuberosume7
- Interactor
- begomovirusBegomoviruse7pathogen
- Outcome
- first observation of a begomovirus causing a severe disease of potatoe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“This is the first observation of a begomovirus causing a severe disease of potato in India”
Claim · model-written summary
The sequence data indicate the cause is a virus closely related to ToLCNDV that, unlike the strain infecting potato, is not sap-transmissible.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- ToLCNDVe1pathogen
- Outcome
- not sap-transmissiblee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“disease of potato in northern India”
e1“The nucleotide sequence data indicate that the cause is a virus closely related to ToLCNDV which, unlike the strain infecting potato, is not sap-transmissible”
Claim · model-written summary
A new strain of ToLCNDV has likely emerged that infects a new host, potato.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- ToLCNDVe0pathogen
- Outcome
- a new strain of ToLCNDV has emerged that infects a new hoste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is likely that a new strain of ToLCNDV has emerged that infects a new host, potato”
Claim · model-written summary
Earlier planting of potato crops may have led to a build-up of whitefly populations and the emergence of a new disease in potato.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- whiteflye0pest
- Outcome
- emergence of a new disease in potatoe0
- Qualifier
- potato crops have been planted earliere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato crops have been planted earlier and this has resulted in a sufficient build-up of whitefly populations that might have led to the emergence of a new disease in potato”
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See all 113 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.