Abstract
Bradyrhizobium japonicum is a soil bacterium that forms nitrogen-fixing nodules on the roots of the agronomically important legume soybean. Microscopic observation of plant roots showed that butanol extract of B. japonicum strain USDA110 cultures induced for nod gene expression elicited root hair deformation, an early event in the nodulation process. The metabolite produced by B. japonicum responsible for root hair deformation activity was purified. Chemical analysis of the compound revealed it to be a pentasaccharide of N-acetylglucosamine modified by a C18:1 fatty acyl chain at the nonreducing end. In these respects, the B. japonicum metabolite is similar to the lipo-oligosaccharide signals described from Rhizobium species. However, the B. japonicum compound is unique in that an additional sugar, 2-O-methylfucose, is linked to the reducing end. Comparative analysis of the B. japonicum Nod metabolite and those characterized from Rhizobium species suggests that the presence of the fucosyl residue plays an important role in the specificity of the B. japonicum-soybean symbiosis. The availability of the purified B. japonicum nodulation signal should greatly facilitate further studies of soybean nodulation.
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Claims 5
Claim · model-written summary
B. japonicum culture extract elicited soybean root hair deformation.
- Taxon
- soybean
Glycine maxt0 - Treatment
- butanol extract of B. japonicum strain USDA110 culturese0
- Interactor
- B. japonicum strain USDA110
Bradyrhizobium japonicume0symbiont - Outcome
- root hair deformatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“butanol extract of B. japonicum strain USDA110 cultures induced for nod gene expression elicited root hair deformation”
t0“legume soybean”
Claim · model-written summary
The active B. japonicum metabolite was purified.
- Taxon
- soybeanGlycine maxt0
- Interactor
- B. japonicumBradyrhizobium japonicume0symbiont
- Outcome
- metabolite produced by B. japonicum responsible for root hair deformation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The metabolite produced by B. japonicum responsible for root hair deformation activity was purified”
t0“legume soybean”
Claim · model-written summary
The signal is a pentasaccharide of N-acetylglucosamine with a C18:1 fatty acyl chain.
- Taxon
- soybeanGlycine maxt0
- Outcome
- pentasaccharide of N-acetylglucosamine modified by a C18:1 fatty acyl chaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a pentasaccharide of N-acetylglucosamine modified by a C18:1 fatty acyl chain at the nonreducing end”
t0“legume soybean”
Claim · model-written summary
The B. japonicum compound has 2-O-methylfucose at its reducing end.
- Taxon
- soybeanGlycine maxt0
- Interactor
- B. japonicumBradyrhizobium japonicume0symbiont
- Outcome
- 2-O-methylfucose, is linked to the reducing ende0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the B. japonicum compound is unique in that an additional sugar, 2-O-methylfucose, is linked to the reducing end”
t0“legume soybean”
Claim · model-written summary
The fucosyl residue may contribute to symbiotic specificity.
- Taxon
- soybeanGlycine maxe0
- Interactor
- B. japonicumBradyrhizobium japonicume0symbiont
- Compound
- fucosyl residuee0
- Outcome
- specificity of the B. japonicum-soybean symbiosise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of the fucosyl residue plays an important role in the specificity of the B. japonicum-soybean symbiosis”
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See all 115 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.