Abstract
BACKGROUND: Developing new population types based on interspecific introgressions has been suggested by several authors to facilitate the discovery of novel allelic sources for traits of agronomic importance. Chromosome segment substitution lines from interspecific crosses represent a powerful and useful genetic resource for QTL detection and breeding programs. RESULTS: We built a set of 64 chromosome segment substitution lines carrying contiguous chromosomal segments of African rice Oryza glaberrima MG12 (acc. IRGC103544) in the genetic background of Oryza sativa ssp. tropical japonica (cv. Caiapó). Well-distributed simple-sequence repeats markers were used to characterize the introgression events. Average size of the substituted chromosomal segments in the substitution lines was about 10 cM and covered the whole donor genome, except for small regions on chromosome 2 and 4. Proportions of recurrent and donor genome in the substitution lines were 87.59% and 7.64%, respectively. The remaining 4.78% corresponded to heterozygotes and missing data. Strong segregation distortion was found on chromosomes 3 and 6, indicating the presence of interspecific sterility genes. To illustrate the advantages and the power of quantitative trait loci (QTL) detection using substitution lines, a QTL detection was performed for scored traits. Transgressive segregation was observed for several traits measured in the population. Fourteen QTLs for plant height, tiller number per plant, panicle length, sterility percentage, 1000-grain weight and grain yield were located on chromosomes 1, 3, 4, 6 and 9. Furthermore, a highly significant QTL controlling resistance to the Rice stripe necrosis virus was located between SSR markers RM202-RM26406 (44.5-44.8 cM) on chromosome 11. CONCLUSIONS: Development and phenotyping of CSSL libraries with entire genome coverage represents a useful strategy for QTL discovery. Mapping of the RSNV locus represents the first identification of a genetic factor underlying resistance to this virus. This population is a powerful breeding tool. It also helps in overcoming hybrid sterility barriers between species of rice.
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Claims 14
Claim · model-written summary
A set of 64 chromosome segment substitution lines of O. glaberrima segments in an O. sativa background was built.
- Taxon
- Oryza sativa
Oryza sativat0 - Material
- cv. Caiapóe1
- Outcome
- chromosome segment substitution linese1
- Value
- 64e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e1“We built a set of 64 chromosome segment substitution lines carrying contiguous chromosomal segments of African rice Oryza glaberrima MG12 (acc. IRGC103544) in the genetic background of Oryza sativa ssp. tropical japonica (cv. Caiapó)”
Claim · model-written summary
The average size of substituted chromosomal segments was about 10 cM.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- Average size of the substituted chromosomal segmentse2
- Value
- about 10 cMe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e2“Average size of the substituted chromosomal segments in the substitution lines was about 10 cM”
Claim · model-written summary
The substitution lines covered the whole donor genome, except small regions on chromosomes 2 and 4.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- donor genomee3
- Qualifier
- except for small regions on chromosome 2 and 4e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e3“covered the whole donor genome, except for small regions on chromosome 2 and 4”
Claim · model-written summary
The recurrent genome proportion in the substitution lines was 87.59%.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- Proportions of recurrent and donor genomee4
- Value
- 87.59%e4
- Qualifier
- recurrente4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e4“Proportions of recurrent and donor genome in the substitution lines were 87.59% and 7.64%, respectively”
Claim · model-written summary
The donor genome proportion in the substitution lines was 7.64%.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- Proportions of recurrent and donor genomee4
- Value
- 7.64%e4
- Qualifier
- donore4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e4“Proportions of recurrent and donor genome in the substitution lines were 87.59% and 7.64%, respectively”
Claim · model-written summary
Strong segregation distortion was found on chromosomes 3 and 6.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- segregation distortione5
- Qualifier
- chromosomes 3 and 6e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e5“Strong segregation distortion was found on chromosomes 3 and 6, indicating the presence of interspecific sterility genes”
Claim · model-written summary
Transgressive segregation was observed for several traits in the population.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- Transgressive segregatione6
- Qualifier
- several traits measured in the populatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e6“Transgressive segregation was observed for several traits measured in the population”
Claim · model-written summary
Fourteen QTLs for plant height, tiller number, panicle length, sterility, 1000-grain weight and grain yield were located on chromosomes 1, 3, 4, 6 and 9.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- QTLse7
- Value
- Fourteene7
- Qualifier
- plant height, tiller number per plant, panicle length, sterility percentage, 1000-grain weight and grain yielde7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e7“Fourteen QTLs for plant height, tiller number per plant, panicle length, sterility percentage, 1000-grain weight and grain yield were located on chromosomes 1, 3, 4, 6 and 9”
Claim · model-written summary
A highly significant QTL controlling resistance to Rice stripe necrosis virus was mapped on chromosome 11 between SSR markers RM202-RM26406.
- Taxon
- Oryza sativaOryza sativat0
- Interactor
- Rice stripe necrosis virusRice stripe necrosis viruse1pathogen
- Outcome
- resistance to the Rice stripe necrosis viruse1
- Value
- 44.5-44.8 cMe1
- Qualifier
- SSR markers RM202-RM26406e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e1“a highly significant QTL controlling resistance to the Rice stripe necrosis virus was located between SSR markers RM202-RM26406 (44.5-44.8 cM) on chromosome 11”
Claim · model-written summary
The mapping of the RSNV locus is the first identification of a genetic factor underlying resistance to this virus.
- Taxon
- Oryza sativaOryza sativat0
- Interactor
- RSNVe1pathogen
- Outcome
- first identification of a genetic factor underlying resistance to this viruse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e1“Mapping of the RSNV locus represents the first identification of a genetic factor underlying resistance to this virus”
Claim · model-written summary
The population is a powerful breeding tool.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- powerful breeding toole1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e1“This population is a powerful breeding tool”
Claim · model-written summary
CSSL libraries with entire genome coverage are a useful strategy for QTL discovery.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- useful strategy for QTL discoverye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e1“Development and phenotyping of CSSL libraries with entire genome coverage represents a useful strategy for QTL discovery”
Claim · model-written summary
The population helps overcome hybrid sterility barriers between rice species.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- overcoming hybrid sterility barrierse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e1“It also helps in overcoming hybrid sterility barriers between species of rice”
Claim · model-written summary
The segregation distortion on chromosomes 3 and 6 indicates interspecific sterility genes.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- segregation distortione5
- Qualifier
- indicating the presence of interspecific sterility genese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e5“Strong segregation distortion was found on chromosomes 3 and 6, indicating the presence of interspecific sterility genes”