Abstract
Plantlets of Curcuma caesia were produced in vitro from newly sprouting vegetative buds of tubers. Segments of the plantlets from the junction between the root and the basal portion of the stem were subsequently used as explants to investigate factors affecting callus induction and plant regeneration via somatic embryogenesis. The explants were placed on Woody Plant Medium (WPM) together with different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and benzyl aminopurine (BAP) in the presence of light. The growth medium supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days of culture. Sub-culturing on the same medium enhanced the production of friable callus. Culture media containing higher concentrations of agar promoted the development of green somatic embryos from the callus. Respond of somatic embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green somatic embryos with an efficiency of 53%. This culture medium also produced the largest number plantlets.
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Claims 5
Claim · model-written summary
Medium with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days.
- Taxon
- Curcuma caesia
Curcuma caesiat0 - Treatment
- 5 mg/L BAP and 2 mg/L 2,4-De0
- Outcome
- callus inductione0
- Direction
- promotede0
- Value
- 70 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth medium supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days of culture.”
t0“Curcuma caesia”
Claim · model-written summary
Subculturing on the same medium enhanced friable callus production.
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- Sub-culturing on the same mediume0
- Outcome
- production of friable calluse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sub-culturing on the same medium enhanced the production of friable callus.”
t0“Curcuma caesia”
Claim · model-written summary
Higher agar concentrations promoted green somatic embryo development.
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- higher concentrations of agare0
- Outcome
- development of green somatic embryose0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culture media containing higher concentrations of agar promoted the development of green somatic embryos from the callus.”
t0“Curcuma caesia”
Claim · model-written summary
MS medium with 6.0 g/L agar, 5 mg/L BAP, and 0.2 mg/L 2,4-D gave the most successful somatic embryogenesis response, with 53% efficiency.
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-De0
- Outcome
- somatic embryogenesise0
- Direction
- most successfule0
- Value
- 53%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Respond of somatic embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green somatic embryos with an efficiency of 53%.”
t0“Curcuma caesia”
Claim · model-written summary
This medium also produced the largest number of plantlets.
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- This culture mediume0
- Outcome
- number plantletse0
- Direction
- largeste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This culture medium also produced the largest number plantlets.”
t0“Curcuma caesia”
The same questions in other papers
2,4-D · growth · Curcuma caesia (black zedoary)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Curcuma caesia (black zedoary)Results agree
2 papers in the same direction as this paper
- positive
- positive
in vitro · growth · Curcuma caesia (black zedoary)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.